Exponential dichotomies of evolution operators in Banach spaces

This paper considers three dichotomy concepts (exponential dichotomy, uniform exponential dichotomy and strong exponential dichotomy) in the general context of non-invertible evolution operators in Banach spaces. Connections between these concepts are illustrated. Using the notion of Green function, we give necessary conditions and sufficient ones for strong exponential dichotomy. Some illustrative examples are presented to prove that the converse of some implication type theorems are not valid

Family dairy farms in the Podlasie province, Poland: farm typology according to farming system

The aim of this paper is to establish a farm typology according to the dairy farming systems in the western part of the Podlasie province. Data of 39 variables was collected by a survey to owners of 123 family farms. A two-stage multivariate analysis was conducted in order to determine farm typology. Three principal components were detected, explaining 80.4% of the total variance. The cluster analysis identified five groups of farms. In two groups the cow productivity is the biggest in the area. A third group contains the smallest and lowest cow productivity farms, with high proportion of non-agricultural activities. One of the two remaining groups has better soil quality and medium cow productivity. The other group has low or medium soil quality but cow productivity is higher than in the fourth group. The SWOT analysis shows different weaknesses and strengths for different groups, as well as those common to a larger number of groups. Weaknesses are related to small farm size, large number of workers, low or medium soil quality and low or medium level of technology. Strengths are related to a large share of fodder crops, low livestock density, diversification of agrarian activities and acceptable cow productivity. On the other hand, general opportunities are linked to the EU-CAP evolution and to the presence of cooperatives in the region, whereas general threats derive from a hypothetic increase of feed prices and quantity of milk produced in the EU, which could lead to a fall in milk prices.El objetivo de este trabajo es establecer tipologías de sistemas lecheros en el oeste de Podlasia (Polonia). Se analizaron 39 variables a partir de encuestas realizadas a propietarios de 123 explotaciones. Tras el análisis multivariante en dos etapas (factorial y cluster) se encontraron tres componentes principales que explican el 80,4% de la varianza total y se obtuvieron cinco grupos de explotaciones. En dos de los grupos la productividad de las vacas es la mayor de la zona. Un tercer grupo tiene las granjas más pequeñas y menos productivas, con una mayor proporción de actividades no agrarias. El cuarto tiene los suelos de mejor calidad y una productividad de las vacas media y el quinto tiene suelos de calidad media o baja pero una productividad de las vacas superior. En general, las debilidades están relacionadas con una escasa dimensión de las granjas, un elevado número de trabajadores, una baja o media calidad de los suelos y un bajo o mediano nivel de tecnología. Las fortalezas están relacionadas con la abundancia de cultivos forrajeros, una carga ganadera baja, una aceptable diversificación agraria y una aceptable productividad de las vacas. Las principales oportunidades están ligadas a la evolución de la PAC de la UE y a la presencia de cooperativas para la comercialización de la leche. Las principales amenazas derivan de los posibles incrementos de precios de los alimentos para el ganado y de leche producida en la UE, que puede conducir a una caída de los precios de venta de la leche

Real-time Optimisation of a Microwave Plasma Gasification System

A microwave plasma gasifier has been designed to produce syngas from waste. Gasification using microwave plasma has various controllable parameters to achieve optimal syngas production. These parameters include the microwave power applied, the reflected power from the microwave plasma jet, the EH tuner arm position, the gas flow and pressure, in addition to the temperature inside the gasifier. A variety of sensors are required to provide feedback and control for each of these parameters. This paper discusses the benefits of gasification, particularly via microwave plasma techniques, the first steps toward the optimisation of such a system and some preliminary results of this optimisation

Dietary habits, physical activity, and sedentary behaviour of children of employed mothers: a systematic review

Since approximately 40% of the global workforce are women, a comprehensive understanding of association of maternal employment with child dietary patterns, physical activity and sedentary behaviour needs more focus. This systematic review aims to identify the association between maternal employment and dietary patterns (DP), physical activity (PA) and sedentary behaviour (SB) of children and adolescents (6 to 18 years). Searches were performed using electronic databases and manual searches. Peer reviewed journal articles, conference papers, theses at masters/doctoral levels in English were included. A total 42 studies met selection criteria, which indicated associations between maternal employment and at least one of the domains of interest: DP, PA and/or SB. Using individual samples of analysis, it was found that, 9 samples of DP, 11 samples of PA and 12 samples of SB were positively correlated with maternal employment, whereas 25 samples of DP, 5 samples of PA and 5 samples of SB showed an opposite association. Results suggest that PA and SB were positively related with maternal employment, whereas DP had an inverse relationship. Findings from this review provide evidence that children of employed mothers had poorer DP and greater prevalence of SB, however, their children are more physically active. Future interventions need to create a positive environment at the workplace and for families to support employed mothers and improve children’s dietary patterns and decrease sedentary behaviours. Future studies should prioritise the domains of DP, PA and SB that have been studied inadequately and have inconsistent results

Genomic Characterization of Methanomicrobiales Reveals Three Classes of Methanogens

BACKGROUND:Methanomicrobiales is the least studied order of methanogens. While these organisms appear to be more closely related to the Methanosarcinales in ribosomal-based phylogenetic analyses, they are metabolically more similar to Class I methanogens. METHODOLOGY/PRINCIPAL FINDINGS:In order to improve our understanding of this lineage, we have completely sequenced the genomes of two members of this order, Methanocorpusculum labreanum Z and Methanoculleus marisnigri JR1, and compared them with the genome of a third, Methanospirillum hungatei JF-1. Similar to Class I methanogens, Methanomicrobiales use a partial reductive citric acid cycle for 2-oxoglutarate biosynthesis, and they have the Eha energy-converting hydrogenase. In common with Methanosarcinales, Methanomicrobiales possess the Ech hydrogenase and at least some of them may couple formylmethanofuran formation and heterodisulfide reduction to transmembrane ion gradients. Uniquely, M. labreanum and M. hungatei contain hydrogenases similar to the Pyrococcus furiosus Mbh hydrogenase, and all three Methanomicrobiales have anti-sigma factor and anti-anti-sigma factor regulatory proteins not found in other methanogens. Phylogenetic analysis based on seven core proteins of methanogenesis and cofactor biosynthesis places the Methanomicrobiales equidistant from Class I methanogens and Methanosarcinales. CONCLUSIONS/SIGNIFICANCE:Our results indicate that Methanomicrobiales, rather than being similar to Class I methanogens or Methanomicrobiales, share some features of both and have some unique properties. We find that there are three distinct classes of methanogens: the Class I methanogens, the Methanomicrobiales (Class II), and the Methanosarcinales (Class III)

Transient and sustained bacterial adaptation following repeated sublethal exposure to microbicides and a novel human antimicrobial peptide

Microbicides (biocides) play an important role in the prevention and treatment of infections. While there is currently little evidence for in-use treatment failures attributable to acquired reductions in microbicide susceptibility, the susceptibility of some bacteria can be reduced by sublethal laboratory exposure to certain agents. In this investigation, a range of environmental bacterial isolates (11 genera, 18 species) were repeatedly exposed to four microbicides (cetrimide, chlorhexidine, polyhexamethylene biguanide [PHMB], and triclosan) and a cationic apolipoprotein E-derived antimicrobial peptide (apoEdpL-W) using a previously validated exposure system. Susceptibilities (MICs and minimum bactericidal concentrations [MBCs]) were determined before and after 10 passages (P10) in the presence of an antimicrobial and then after a further 10 passages without an antimicrobial to determine the stability of any adaptations. Bacteria exhibiting >4-fold increases in MBCs were further examined for alterations in biofilm-forming ability. Following microbicide exposure, ≥4-fold decreases in susceptibility (MIC or MBC) occurred for cetrimide (5/18 bacteria), apoEdpL-W (7/18), chlorhexidine (8/18), PHMB (8/18), and triclosan (11/18). Of the 34 ≥4-fold increases in the MICs, 15 were fully reversible, 13 were partially reversible, and 6 were nonreversible. Of the 26 ≥4-fold increases in the MBCs, 7 were fully reversible, 14 were partially reversible, and 5 were nonreversible. Significant decreases in biofilm formation in P10 strains occurred for apoEdpL-W (1/18 bacteria), chlorhexidine (1/18), and triclosan (2/18), while significant increases occurred for apoEdpL-W (1/18), triclosan (1/18), and chlorhexidine (2/18). These data indicate that the stability of induced changes in microbicide susceptibility varies but may be sustained for some combinations of a bacterium and a microbicide

Connecting Peptide Physicochemical and Antimicrobial Properties by a Rational Prediction Model

The increasing rate in antibiotic-resistant bacterial strains has become an imperative health issue. Thus, pharmaceutical industries have focussed their efforts to find new potent, non-toxic compounds to treat bacterial infections. Antimicrobial peptides (AMPs) are promising candidates in the fight against antibiotic-resistant pathogens due to their low toxicity, broad range of activity and unspecific mechanism of action. In this context, bioinformatics' strategies can inspire the design of new peptide leads with enhanced activity. Here, we describe an artificial neural network approach, based on the AMP's physicochemical characteristics, that is able not only to identify active peptides but also to assess its antimicrobial potency. The physicochemical properties considered are directly derived from the peptide sequence and comprise a complete set of parameters that accurately describe AMPs. Most interesting, the results obtained dovetail with a model for the AMP's mechanism of action that takes into account new concepts such as peptide aggregation. Moreover, this classification system displays high accuracy and is well correlated with the experimentally reported data. All together, these results suggest that the physicochemical properties of AMPs determine its action. In addition, we conclude that sequence derived parameters are enough to characterize antimicrobial peptides

Sap Transporter Mediated Import and Subsequent Degradation of Antimicrobial Peptides in Haemophilus

Antimicrobial peptides (AMPs) contribute to host innate immune defense and are a critical component to control bacterial infection. Nontypeable Haemophilus influenzae (NTHI) is a commensal inhabitant of the human nasopharyngeal mucosa, yet is commonly associated with opportunistic infections of the upper and lower respiratory tracts. An important aspect of NTHI virulence is the ability to avert bactericidal effects of host-derived antimicrobial peptides (AMPs). The Sap (sensitivity to antimicrobial peptides) ABC transporter equips NTHI to resist AMPs, although the mechanism of this resistance has remained undefined. We previously determined that the periplasmic binding protein SapA bound AMPs and was required for NTHI virulence in vivo. We now demonstrate, by antibody-mediated neutralization of AMP in vivo, that SapA functions to directly counter AMP lethality during NTHI infection. We hypothesized that SapA would deliver AMPs to the Sap inner membrane complex for transport into the bacterial cytoplasm. We observed that AMPs localize to the bacterial cytoplasm of the parental NTHI strain and were susceptible to cytoplasmic peptidase activity. In striking contrast, AMPs accumulated in the periplasm of bacteria lacking a functional Sap permease complex. These data support a mechanism of Sap mediated import of AMPs, a novel strategy to reduce periplasmic and inner membrane accumulation of these host defense peptides