178 research outputs found
Cellular senescence at the crossroads of inflammation and Alzheimer's disease
Aging is a key risk factor for Alzheimer's disease (AD), but the reasons for this association are not well understood. Senescent cells accumulate in aged tissues and have been shown to play causal roles in age-related pathologies through their proinflammatory secretome. The question arises whether senescence-induced inflammation might contribute to AD and bridge the gap between aging and AD. Here, we highlight the role of cellular senescence as a driver of the aging phenotype, and discuss the current evidence that connects senescence with AD and neurodegeneration
Aplicación de la genética e innovaciones biotecnológicas en el aprovechamiento sostenible de los recursos del mar
Las Ciencias del Mar tienen como objetivo central poder entender los procesos físicos y biológicos que ocurren en este ambiente. La información obtenida es el insumo básico para el diagnóstico, planificación y uso sostenible de los recursos del mar para el beneficio humano, en el presente y el futuro. Para efectos prácticos, los organismos vivos son genotipos que se expresan en un determinado ambiente, y si bien desde la primera mitad del siglo pasado conocemos los mecanismos básicos de la transmisión hereditaria, no es sino hasta la segunda mitad del siglo pasado que ha sido posible acceder directamente al genoma de los organismos
Phenomenology of a scotogenic neutrino mass model at 3-loops
By extending the minimal scotogenic model with a spontaneously broken global
symmetry and a preserved symmetry, we build a seesaw
model for generating neutrino masses at three-loop level. The new particles
have masses at the TeV scale and relatively large Yukawa couplings, which leads
to sizable rates for charged lepton flavor violation processes, well within
future experimental reach. The model is able to successfully explain the
mass anomaly and provides a viable fermionic or scalar dark matter candidate,
while satisfying all current constraints imposed by neutrinoless double-beta
decay, charged-lepton flavor violation, and electroweak precision observables.Comment: 4 pages, 3 figures. Presented at the XVIII International Conference
on Topics in Astroparticle and Underground Physics (TAUP 2023
New distance measures for classifying X-ray astronomy data into stellar classes
The classification of the X-ray sources into classes (such as extragalactic
sources, background stars, ...) is an essential task in astronomy. Typically,
one of the classes corresponds to extragalactic radiation, whose photon
emission behaviour is well characterized by a homogeneous Poisson process. We
propose to use normalized versions of the Wasserstein and Zolotarev distances
to quantify the deviation of the distribution of photon interarrival times from
the exponential class. Our main motivation is the analysis of a massive dataset
from X-ray astronomy obtained by the Chandra Orion Ultradeep Project (COUP).
This project yielded a large catalog of 1616 X-ray cosmic sources in the Orion
Nebula region, with their series of photon arrival times and associated
energies. We consider the plug-in estimators of these metrics, determine their
asymptotic distributions, and illustrate their finite-sample performance with a
Monte Carlo study. We estimate these metrics for each COUP source from three
different classes. We conclude that our proposal provides a striking amount of
information on the nature of the photon emitting sources. Further, these
variables have the ability to identify X-ray sources wrongly catalogued before.
As an appealing conclusion, we show that some sources, previously classified as
extragalactic emissions, have a much higher probability of being young stars in
Orion Nebula.Comment: 29 page
Epithelial immune activation and intracellular invasion by non-typeable Haemophilus influenzae
Type-2 low asthma affects 30-50% of people with severe asthma and includes a phenotype characterized by sputum neutrophilia and resistance to corticosteroids. Airways inflammation in type-2 low asthma or COPD is potentially driven by persistent bacterial colonization of the lower airways by bacteria such as non-encapsulated Haemophilus influenzae (NTHi). Although pathogenic in the lower airways, NTHi is a commensal of the upper airways. It is not known to what extent these strains can invade airway epithelial cells, persist intracellularly and activate epithelial cell production of proinflammatory cytokines, and how this differs between the upper and lower airways. We studied NTHi infection of primary human bronchial epithelial cells (PBECs), primary nasal epithelial cells (NECs) and epithelial cell lines from upper and lower airways. NTHi strains differed in propensity for intracellular and paracellular invasion. We found NTHi was internalized within PBECs at 6 h, but live intracellular infection did not persist at 24 h. Confocal microscopy and flow cytometry showed NTHi infected secretory, ciliated and basal PBECs. Infection of PBECs led to induction of CXCL8, interleukin (IL)-1β, IL-6 and TNF. The magnitude of cytokine induction was independent of the degree of intracellular invasion, either by differing strains or by cytochalasin D inhibition of endocytosis, with the exception of the inflammasome-induced mediator IL-1β. NTHi-induced activation of TLR2/4, NOD1/2 and NLR inflammasome pathways was significantly stronger in NECs than in PBECs. These data suggest that NTHi is internalized transiently by airway epithelial cells and has capacity to drive inflammation in airway epithelial cells
Global metabolomic profiling of uterine leiomyomas
Background: Uterine leiomyomas can be classified into molecularly distinct subtypes according to their genetic triggers: MED12 mutations, HMGA2 upregulation, or inactivation of FH. The aim of this study was to identify metabolites and metabolic pathways that are dysregulated in different subtypes of leiomyomas. Methods: We performed global metabolomic profiling of 25 uterine leiomyomas and 17 corresponding myometrium specimens using liquid chromatography-tandem mass spectroscopy. Results: A total of 641 metabolites were detected. All leiomyomas displayed reduced homocarnosine and haeme metabolite levels. We identified a clearly distinct metabolomic profile for leiomyomas of the FH subtype, characterised by metabolic alterations in the tricarboxylic acid cycle and pentose phosphate pathways, and increased levels of multiple lipids and amino acids. Several metabolites were uniquely elevated in leiomyomas of the FH subtype, including N6-succinyladenosine and argininosuccinate, serving as potential biomarkers for FH deficiency. In contrast, leiomyomas of the MED12 subtype displayed reduced levels of vitamin A, multiple membrane lipids and amino acids, and dysregulation of vitamin C metabolism, a finding which was also compatible with gene expression data. Conclusions: The study reveals the metabolomic heterogeneity of leiomyomas and provides the requisite framework for strategies designed to target metabolic alterations promoting the growth of these prevalent tumours.Peer reviewe
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