4,352 research outputs found

    In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology

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    Cryo-electron tomography is a powerful technique that can faithfully image the native cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo-electron tomography provides a label-free method of detecting biological structures, relying on the intrinsic contrast of frozen cellular material for direct identification of macromolecules. Recent advances in sample preparation, detector technology, and phase plate imaging have enabled the structural characterization of protein complexes within intact cells. Here, we review these technical developments and outline a detailed computational workflow for in situ structural analysis. Two recent studies are described to illustrate how this workflow can be adapted to examine both known and unknown cellular complexes. The stage is now set to realize the promise of visual proteomics a complete structural description of the cell's native molecular landscape. (C) 2015 Elsevier Ltd. All rights reserved

    Labour Market and Social Policy in Italy: Challenges and Changes. Bertelsmann Policy Brief #2016/02

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    vEight years after the outbreak of the financial crisis, Italy has still to cope with and overcome a plethora of economic and social challenges. On top of this, it faces an unfavourable demographic structure and severe disparities between its northern and southern regions. Some promising reforms have recently been enacted, specifically targeting poverty and social exclusion. However, much more remains to be done on the way towards greater economic stability and widely shared prosperity

    Stereotypes and prejudice in the blood: sucrose drinks reduce prejudice and stereotyping

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    "Prejudice and stereotyping cause social problems and intergroup tension. The current work examined whether bolstering self-control by giving participants glucose would reduce stereotype use for an impression formation task. Previous work has demonstrated that self-control depends on biologically expensive brain processes that consume energy derived from glucose in the bloodstream. In the current study, glucose was manipulated via lemonade sweetened with either sugar or Splenda. Compared to the control group, the participants in the glucose condition used fewer stereotypes when writing an essay about a day in the life of a gay man. In addition, high-prejudice participants in the glucose condition used fewer derogatory statements in their essays than high-prejudice participants in the control condition. The findings are discussed in terms of the importance of self-control resources in the effective regulation of prejudice and stereotyping." [author's abstract
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