Characteristics of epstein barr virus variants associated with gastric carcinoma in Southern Tunisia

<p>Abstract</p> <p>Backgroud</p> <p>EBV-associated Gastric Carcinoma (EBVaGC) has a distinct clinical features and its prevalence is variable worldwide.</p> <p>Results</p> <p>To determine the prevalence of EBVaGC in Tunisia, EBV-encoded small RNA (EBER) expression was assessed in 81 gastric carcinoma (GC) specimens. The nuclear EBER expression was detected in 12 out of 81 GC cases (14.81%) and concordance between the score range of EBER staining and the number of EBV DNA copies as estimate by QPCR is observed. On the other hand, we found that EBVaGC strongly correlated with age at diagnosis, and weakly with tumor differentiation and venous invasion.</p> <p>Furthermore, the EBVaGC specimens were subjected to determine the EBV DNA polymorphisms. Our results show a unique genetic profile of the EBV strains regarding the A and D types, the F prototype, the retention of <it>Xho</it>I restriction site and the 30 bp del-LMP1 variant. <b><it>According to our previous studies on nasopharyngeal carcinoma (NPC), we suggested that EBV strains associated to GC and NPC shared some similarities in Tunisian patients</it>.</b></p> <p>Conclusion</p> <p>The prevalence of EBVaGC is of 14.81% in the southern Tunisia and that common EBV strain are associated with both NPC and GC which are likely to differ from Asian strains. Our findings support therefore a certain geographical distribution of EBV strains which is not restricted to EBV-associated malignancies.</p

Multi-criteria/comparative analysis and multi-objective optimization of a hybrid solar/geothermal source system integrated with a carnot battery

Among the different electrical energy storage technologies, the Carnot batteries are promising options with low specific cost that do not suffer from geographical limitations and power-capacity coupling. In addition to power balancing, this approach can also be unique for multi-vector energy management. A comprehensive evaluation (thermodynamic design and exergoenvironmental and exergoeconomic evaluations), comparison, and multi-objective optimization of four Carnot battery configurations based on solar-electric energy and a geothermal source is presented. Geothermal energy can simultaneously improve the thermodynamic and environmental performances of the Carnot battery. The main structure of all configurations is based on electrical energy obtained from PV and captured thermal energy from a geothermal source. The four Brayton, heat pump, flash, and organic Rankine cycle (ORC) units are periodically integrated. The outcomes point out that the discharging process is based on an ORC unit and a flash-heat pump cycle (F-HPC)-based charging process makes more optimal heat-to-power efficiency. Moreover, the Carnot battery based on the regenerative-Brayton cycle (R-BC) unit has a higher investment cost rate compared to the ORC unit (in the discharging process). When integrating the geothermal, the third configuration (R-HPC/R-BC) experiences the greatest improvement (5.3-fold) due to the increase in thermal energy received from the geothermal source

Numerical Study of a Phase Change Material Energy Storage Tank Working with Carbon Nanotube–Water Nanofluid under Ha’il City Climatic Conditions

A numerical investigation of a phase change material (PCM) energy storage tank working with carbon nanotube (CNT)–water nanofluid is performed. The study was conducted under actual climatic conditions of the Ha’il region (Saudi Arabia). Two configurations related to the absence or presence of conductive baffles are studied. The tank is filled by encapsulated paraffin wax as the PCM, and CNT–water nanofluid flows through the capsules. The main goal is to increase the temperature of the PCM to 70 °C in order to store the thermal energy, which can then be used during the night and cloudy weather. Numerical computations are made using the finite element method (FEM) based on actual measured weather conditions. Climate conditions were collected from a weather station located on the roof of the engineering college’s building at the University of Ha’il. The collected data served as input to the numerical model, and the simulations were performed for three months (December, March, and July). The solid CNT volume fraction range was (0 ≤ ϕ ≤ 0.05) and the nanofluid volume flow rate ranged was (0.5 L/min ≤ V ≤ 3 L/min). For both considered cases (with and without baffles), it was found that the use of CNT–nanofluid led to a reduction in the charging time and enhanced its performance. An increase in the volumetric flow rate was found to accelerate the melting process. The best performances of the storage tank occurred during July due to the highest solar irradiation. Furthermore, it was found that the use of baffles had no beneficial effects on the melting process

FOXA1 Expression in Nasopharyngeal Carcinoma: Association with Clinicopathological Characteristics and EMT Markers

The forkhead box (FOXA) family of transcription factors regulates gene expression and chromatin structure during tumorigenesis and embryonic development. Until now, the relationship between FOXA1 and the nasopharyngeal carcinoma (NPC) has not yet been reported. Therefore, our purpose is to analyze the expression of FOXA1 in 56 NPC patients compared to 10 normal nasopharyngeal mucosae and to correlate the expression with the clinicopathological features. Besides, we investigated the association between FOXA1 and LMP1 gene expression, as well as the EMT markers namely the E-cadherin and Twist1. Among 56 NPC tissues, 34 (60.7%) cases were positive for FOXA1. Furthermore, we noticed that FOXA1 expression correlated with TNM (p=0.037), and age at diagnosis (p=0.05). Moreover, positive expression of FOXA1 is likely to be associated with prolonged disease-free survival and overall survival rates. On the other hand, we observed a positive association between the expression of E-cadherin and FOXA1 (p=0.0051) whereas Twist1 correlated negatively with FOXA1 (p=0.004). Furthermore, knowing that LMP1 plays a key role in the pathogenesis of NPC, we explored the association of FOXA1 with the LMP1 gene expression in both NPC cell lines and tissues. We found that, in the C666-1 which displays low levels of LMP1, the expression of FOXA1 is high, and inversely in the C15 cell line that expresses a high level of LMP1, the level of FOXA1 is low. Besides, in accordance to our results, we found that in NPC tissues there is a negative association between LMP1 and FOXA1. In conclusion, our results suggest that the overexpression of FOXA1 is associated with a nonaggressive behavior and favorable prognosis in NPC patients. FOXA1 could contribute in the EMT process through key factors as E-cadherin, Twist1, and LMP1

Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma: TTC40

To further explore the epigenetic changes in nasopharyngeal carcinoma (NPC), methylation-sensitive arbitrarily primed PCR was performed on NPC biopsies and nontumor nasopharyngeal samples. We have shown mainly two DNA fragments that appeared to be differentially methylated in NPCs versus nontumors. The first, defined as hypermethylated, corresponds to a CpG island at the 5′-end of the tetratricopeptide repeat domain 40 (TTC40) gene, whereas the second, defined as hypo-methylated, is located on repetitive sequences at chromosomes 16p11.1 and 13.1. Thereafter, the epigenetic alteration on the 5′-TTC40 gene was confirmed by methylation-specific PCR, showing a significant aberrant methylation in NPCs, compared to nontumors. In addition, the bisulfite sequencing analysis has shown a very high density of methylated cytosines in C15, C17, and X666 NPC xenografts. To assess whether TTC40 gene is silenced by aberrant methylation, we examined the gene expression by reverse transcription-PCR. Our analysis showed that the mRNA expression was significantly lower in tumors than in nontumors, which is associated with 5′-TTC40 gene hypermethylation. In conclusion, we found that the 5′-TTC40 gene is frequently methylated and is associated with the loss of mRNA expression in NPCs. Hypermethylation of 5′-TTC40 gene might play a role in NPC development; nevertheless, other studies are needed

B1.12: a novel peptide interacting with the extracellular loop of the EBV oncoprotein LMP1

Abstract Latent membrane protein 1 (LMP1) encoded by the Epstein-Barr virus (EBV) plays an important role in EBV-induced cell transformation. Down-regulation of the LMP1 expression had shown promising results on cancer cell therapy. In this study, we identified by Phage display a novel peptide called B1.12 (ACPLDLRSPCG) which selectively binds to the extracellular loop (B1) of the LMP1 oncoprotein as demonstrated by molecular docking, NMR and ITC. Using an LMP1 expressing cell line, we showed that B1.12 decreased cell viability, and induced G0/G1 cell cycle arrest. In addition, the expression of A20, pAkt, and pNFkb (pRelA536) in C666-1 cells treated with B1.12 decreased compared to the untreated cells. In conclusion, we selected a novel peptide able to bind specifically to the extracellular loop of LMP1 and thus modulate its oncogenic properties