HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds

A new HPLC optimized method for simultaneous determination of some mycotoxins by separation and using a Dionex UltiMate3000 modular system, with multichannel UV detector was developed and validated. The method optimization was performed to determine simultaneously, several relevant mycotoxins from corn seeds that were stored over 8 months. The mycotoxins selected for quantification were: aflatoxin B1, ochratoxin A and zearalenone. They were selected due to their high concentration in the fodder. For the development stages of the HPLC method, it was introduced an internal standard to have accurate results. Taking into account that the analytes should be extracted from the complex matrix they reside, an extraction procedure was performed, using organic solvents, and the selection after repeated tests demonstrated the best capacity for ethyl acetate. The recovery is about 60–70 % after the extraction process also there is a good preconcentration (×2.5) of the analytes and the internal standard for their quantification. The limit of quantification (LOQ) obtained by chromatographic parameters optimization, for aflatoxin B1 and ochratoxin A were about 3–5 μg kg–1 and 14.4 μg kg–1 for zearalenone of raw biological material, making these values lower than those accepted by the actual normatives and regulations. (doi: 10.5562/cca1788

Evaluation of the Venous System of Wistar Rat Liver by Injection with Epoxy Resin

The rat is the most widely used experimental model in surgical research due to several factors, including that it is easy to handle and inexpensive. It can be used in investigations related to liver regeneration, liver metastases, or transplant immunology. This study highlights the venous components of the hepatic circulation in the Wistar rat by intravascular injection of a polymer that allows the assessment of the distribution of vessels, regardless of their caliber. Five cadavers of 11-month-old male Wistar rats from the USAMV Cluj-Napoca biobase destined for incineration, were used to highlight the liver venous system. A dye mixture, consisting of epoxy resin-catalyst-blue acrylic dye in a 2:1:1 ratio, was injected. After 24 hours, biological tissues were macerated by immersion in 10% KOH solution for five days. The intrahepatic venous system is represented by the venous branches that continue into the liver lobes. Those vessels follow a parallel trajectory with the hepatic artery branches represented by the right and left ramifications. The right portal vein presents branches to the lateral and medial parts of the right lobe of the liver and the caudate lobe. The left branch of the portal vein has ramificationsfor the lateral and medial parts of the left lobe and the quadrate lobe. The technique of injecting the venous circulation of the liver, followed by tissue maceration, allowed the removal by anatomical dissection of all liver components that permits the identification of all components of the venous system, including the finest venous branches of the hepatic circulation

Rationale and design of the MULTISTARS AMI Trial: a randomized comparison of immediate versus staged complete revascularization in patients with ST-segment elevation myocardial infarction and multivessel disease

Background: About half of patients with acute ST-segment elevation myocardial infarction (STEMI) present with multivessel coronary artery disease (MVD). Recent evidence supports complete revascularization in these patients. However, optimal timing of non-culprit lesion revascularization in STEMI patients is unknown because dedicated randomized trials on this topic are lacking. Study design: The MULTISTARS AMI trial is a prospective, international, multicenter, randomized, two-arm, open-label study planning to enroll at least 840 patients. It is designed to investigate whether immediate complete revascularization is non-inferior to staged (within 19-45 days) complete revascularization in patients in stable hemodynamic conditions presenting with STEMI and MVD and undergoing primary percutaneous coronary intervention (PCI). After successful primary PCI of the culprit artery, patients are randomized in a 1:1 ratio to immediate or staged complete revascularization. The primary endpoint is a composite of all-cause death, non-fatal myocardial infarction, ischemia-driven revascularization, hospitalization for heart failure, and stroke at 1 year. Conclusions: The MULTISTARS AMI trial tests the hypothesis that immediate complete revascularization is non-inferior to staged complete revascularization in stable patients with STEMI and MVD

Simulation of Dynamic Operation in Salient Pole Synchronous Machines

The design of today's high performance synchronous motors must take into account not only the steady state but also practically inevitable dynamic regimes. In such conditions an accurate predetermination of the parameters and constructive solution by modeling and simulation of the given dynamic operation, becomes a mandatory step. In this aim, in the present paper a novel mathematical model of the high power salient pole synchronous machine (SPSM) is elaborated. We proposed to consider especially the effects of the magnetic saturation and saliency. The qualitative and quantitative results show an important number of valuable information in the design stage of the synchronous machine