Protective roles of Cimin grape tissues on oxidative stress markers in the cellular system model

Aim: To analyze the phenolic composition of the Cimin grape, which is 1 of 2 grape varieties with a protected geographical indication in Turkey and is used locally to treat the symptoms of some disorders such as impotence and cardiovascular diseases, and to investigate its antioxidant potency against oxidant mediators in the models of intra- and extracellular surroundings. Materials and methods: Cimin grape samples were classified into 5 groups according to the grape’s tissues and the extraction solvents used. Free radical scavenging (DPPH) and antilipid peroxidation product [thiobarbituric acid reactive substance (TBARS) and conjugated diene (CD)] levels of the grape tissues were extrapolated from the measurement of total phenolic and individual monomeric flavanol contents in each extract. Results: The seed methanolic extract had the highest total amount of flavanols, with the amount of catechin at 4.034 mM. The DPPH activities of the seed extracts were 2- to 10-fold higher than those of the other samples. The seed extract-treated erythrocyte and unfractionated human plasma also showed lower TBARS and CD values. In addition, regeneration of glutathione was more obvious in grape seed extracts than in the rest of the tissues. Conclusion: The underlying mechanism of these changes can be related mainly to increased antioxidant status. Cimin grape consumption may have beneficial effects on health maintenance

The effectiveness of electro-acupuncture in ovarian ischemia reperfusion injury

Electro-acupuncture is widely used in gynecology. Adnexal torsion is an important threat to ovarian reserves in women of reproductive age. This is the first study to investigate whether electro-acupuncture is beneficial in protecting ovarian reserves in case of adnexal torsion resulting in reperfusion injury. Thirty-two female Wistar Albino rats were randomized into four groups: the first group-sham operation, the second group-torsion/detorsion model, the third group-pre-acupunture + torsion/detorsion + post-acupuncture, and the fourth group-torsion/detorsion + post-acupuncture. The acupoints used were CV4 and bilateral SP6, Ex-CA1, Kid3, and ST36. In the third group, the acupoints were needled for two weeks before torsion, continuing for a further two weeks after torsion. In the fourth group, needling began after torsion and was maintained for two weeks. Both histological and biochemical parameters indicating ovarian reserves showed that electro-acupuncture applied to the above points exhibited an ameliorating effect on ovaries injured during ischemia/reperfusion. Electro-acupuncture may be capable of protecting against and preventing ischemia/reperfusion injury in case of ovarian torsion

The effect of white tea on serum TNF-α/NF-κB and immunohistochemical parameters in cisplatin-related renal dysfunction in female rats

The study was funded by a grant from the scientific research foundation of Recep Tayyip Erdogan University .Objective: Nephrotoxicity is the most important side effect of the antineoplastic drug cisplatin, thereby restricting its use. The aim of this study was to investigate the protective effects of white tea infusions (WT) against renal damage induced by cisplatin (CP) in rats by biochemical and histopathological means. Materials and methods: This study used 24 female Sprague Dawley rats at 12–14 weeks of age and weighing 250–300 g. Rats were divided into three groups: Control, CP and CP + WT groups. CP was injected 7 mg/kg i.p as a single dose/rat in the CP group. White tea was given at a dose of 0.5% (w/v) for 4 weeks. At the end of the experiment, blood urea nitrogen (BUN), creatinine, uric acid, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and nuclear factor kappa B (NF-κB) along with caspase-3 in the kidney were evaluated in study. Results: BUN, creatinine, TNF-α NF-κB and IL-6 levels of the CP group showed a statisically significant increase in comparison to the control group. TNF-α NF-κB and IL-6 levels showed a statistically significant decrease in the CP + WT group with respect to the CP group. Caspase-3 levels in tubular epithelial cells decreased in CP + WT group compared with CP group (p = 0.02). Conclusion: White tea infusions reduced significantly the nephrotoxicity of CP. The anti-nephrotoxic feature of the infusion may be attributed primarily to its anti-inflammatory and anti-apoptotic characteristics

Comparison of inflammatory biomarkers for detection of coronary stenosis in patients with stable coronary artery disease

Erdogan, Turan/0000-0003-2986-5457; Cetin, Mustafa/0000-0001-6342-436X; yilmaz, adnan/0000-0003-4842-1173WOS: 000319223400015PubMed: 23329531BACKGROUND: the objective of the current study was to evaluate the role of various inflammatory biomarkers in detection of coronary stenosis in patients with stable coronary artery disease (CAD) and healthy people. METHODS: A total of 111 patients with stable coronary artery disease, and 66 healthy subjects were enrolled in the study. Serum levels of lipoprotein-associated-phospholipase A2 (Lp-PLA(2)), high-sensitivity C-reactive protein (hs-CRP), and myeloperoxidase (MPO) were measured to compare patient and control groups. RESULTS: Baseline characteristics were similar between healthy and patient groups, with the exception of age. ANCOVA and log-transformed data of inflammatory biomarkers revealed that, Lp-PLA(2) (p < 0,001) and hs-CRP (p < 0,05) levels in all patient groups were significantly higher than in the control group. Conversely, there was no significant difference in MPO levels among groups. CONCLUSIONS: in stable CAD patients, serum Lp-PLA(2) levels are more compatible than hsCRP and MPO levels in the detection of coronary stenosis.PfizerPfizer; ServierServier; Astra ZenecaAstraZenecaThis study was supported by the drug companies Pfizer, Servier and Astra Zeneca. the Authors thank all of the companies that helped during this investigation

Investigation of relationship between a non-coding rs599839 variant associated with sortilin (Sort1) gene region and lipoprotein subfractions in patients with coronary artery disease

Bu çalışmada Sort1 gen ekspresyonunun lipoprotein altsınıfları ve KAH ile ilişkisini incelendi. Ayrıca Sort1 ile aynı lokusta yer alan ve genin ekspresyonunu etkileyen rs599839 polimorfizmi çalışıldı. Çalışma grupları; anjiyografi sonucu çeşitli düzeylerde damar tıkanıklığı tespit edilen 162 hasta ve 49 sağlıklı bireylerden oluşturulmuştur. Çalışma gruplarında lipit profilleri (TAG, TK, LDL, HDL), serum apolipoprotein (ApoA, ApoB ve ApoE) seviyeleri ve CRP değeri otoanalizörde çalışıldı. Lipoproten altsınıfları Qantimetrix lipoprint sistem kullanılarak analiz edildi. Sort1 gen ekspresyonu düzeyi ise Human Sortilin TaqMan Gene Expression Assays kullanılarak qRT-PCR metoduyla ile belirlendi. Genomik DNA tam kandan izole edildi ve rs599839 polimorfiziminin belirlenmesi için genotipleme Polimeraz Zincir Reaksiyonu-Restiriksiyon Fragment Uzunluk Polimorfizmi (PCR-RFLP) metadu ile çalışıldı. Kontrol grubuna göre KAH grubunda Sort1 gen ekspresyonu anlamlı derecede artmıştır (p0,05). Çalışma grubunda lipoprotein altsınıfları karşılaştırıldığında; kontrol grubuna göre KAH'da aterojenik altfraksiyonlar (SdLDL-K ve SHDL) istatistiksel olarak daha yüksek bulunmuştur (p 0,05) were identified in Sort1 gene expression between variant allele groups of rs599839. When the lipoprotein subclasses were evaluated in the study groups; atherogenic subfractions (SdLDL-K and SHDL) were found to be statistically higher in CAD than control group (p <0,01). Patients with CAD had significantly higher levels of plasma atherogenic index (AIP), TG / HDL-C, Apo B / Apo AI, SdLDL / LbLDL and low LDL-C / Apo B levels compared to the controls (p <0,05). In conclusion, it has been found that Sort1 gene expression particularly has a linear relationship with both the atherogenic LDL phenotype and CAD

Effect of white tea consumption on serum leptin, tnf-alpha and body weight in menopausal model rats

This work was supported by RTEU-BAP, TSA-2017-816.[No Asbtact Available]Turkish Neuroendocrinol So

Effect of White Tea Consumption on Serum Leptin, TNF-alpha and Body Weight in Menopausal Model Rats

3rd International Congress of the Turkish-Neuroendocrinology-Society -- JUN 29-JUL 01, 2018 -- Malatya, TURKEYWOS: 000445952400046[No abstract available]Turkish Neuroendocrinol SocRTEU-BAPRecep Tayyip Erdogan University [TSA-2017-816]This work was supported by RTEU-BAP, TSA-2017-816

An investigation of the effects of metformin on ovarian ischemia-reperfusion injury in rats

Mercantepe, Tolga/0000-0002-8506-1755WOS: 000500178300003PubMed: 31730761Damage to the ovaries or tissue torsion can significantly reduce the ovarian reserve and thus cause severe gynecological and hormonal deficiencies. the discovery of new agents is always needed in the treatment of this condition. Metformin (MET) has been shown to be beneficial in attenuating ovarian ischemia-reperfusion injury. Fifty-six female Sprague Dawley rats were divided into seven groups. Group 1 represented the control group (C), Group 2, the ischemia group (I), and Group 3, the ischemia/reperfusion group (I/R). Group 4, the ischemia (I) + 250 group, and Group 5, the ischemia (I) + 500 group, received 250 mg/kg and 500 mg/kg MET, respectively. Group 6, the ischemia/reperfusion (I/R) + 250 group, and Group 7, the ischemia/reperfusion (I/R) + 500 group, received 250 mg/kg and 500 mg/kg MET, respectively. Tissue malondialdehyde (MDA), glutathione (GSH), and tumor necrosis factor-alpha (TNF-alpha) levels in ovarian tissue increased following I/R, while estradiol (E-2) levels decreased. Moreover, infiltration and diffuse edematous areas were observed in addition to diffuse vascular congestion and hemorrhage findings. Caspase-3 and nuclear factor kappa B (NF-kappa beta) expression levels also increased. MDA and TNF-alpha concentrations decreased in the MET treatment groups, while GSH and E-2 levels increased. the findings showed that I/R causes ovarian damage through the induction of oxidative stress, inflammation, and apoptosis. However, MET application was effective in preventing damage in ovarian tissue by reducing levels of reactive oxygen species, proinflammatory cytokines, caspase-3 and NF-kappa beta.Scientific Research Projects Unit of Recep Tayyip Erdogan University [TSA-2017-774]This work was supported by Scientific Research Projects Unit of Recep Tayyip Erdogan University [grant numbers TSA-2017-774]

Effect of white tea consumption on serum leptin, TNF-α and UCP1 gene expression in ovariectomized rats

Background: Obesity and dyslipidemia due to estrogen deficiency are among the important health problems in menopausal women. Increasing evidence reports the anti-obesity and anti-hyperlipidemic properties of tea polyphenols. However, the effect of white tea (WT) with high polyphenol content on overweight and lipid profile is uncertain. Here, we aimed to examine the effects of long-term WT consumption on serum leptin, tumor necrosis factor- alpha (TNF-α) and uncoupling protein 1 (UCP1) mRNA gene expression in ovariectomized (OVX) rats. Methods: Adult rats were divided into four groups (n = 8): (i) sham, (ii) OVX, (iii) WT and (iv) OVX + WT. WT was given at a dose of 0.5% w/v for 12 weeks. In the study, body weight, serum leptin, TNF, estradiol (E2) levels, lipid profile and UCP1 mRNA gene expression in brown adipose tissue (BAT) were evaluated. Results: There was a significant increase in body weight of OVX rats, which was decreased following WT consumption. While leptin and E2 levels decreased in the OVX group, TNF levels increased. There was no difference between the NF-κB levels of the groups. In addition, BAT UCP1 mRNA expression was significantly decreased in OVX groups, while WT treatment stimulated UCP1 activity. Conclusion: We explain the stimulatory effect of WT on weight loss mainly by the induction of UCP1 gene-mediated thermogenesis and suppression of inflammation. Therefore, we suggest that prolonged WT consumption may have beneficial effects in limiting excess weight gain caused by estrogen deficiency