Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS)

Introduction: Chrysin is a natural flavonoid antioxidant which its role in tumor cell death has been reported. The aim of this study was to investigate the mechanism of Chrysin effect on proliferation and apoptosis of gastric cancer cell line (AGS). Methods: Cells were cultured and treated with different concentrations of Chrysin (90, 80, 70, 60, 50 &mu;M) for 48 h and evaluated for cell viability. To examine the cytotoxic effect of drug in inducing apoptosis, staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) was used. The cells were treated for 48 h with different concentrations of Chrysin (50 of 90 &mu;M) and examined for the morphological changes. The one-way analysis of variance (ANOVA) and the Excel software were used for data analysis. Results: Different concentrations of Chrysin significantly inhibited the growth and proliferation of AGS cells (p&lt;0.05). The IC50 dose was determined to be 67.5&plusmn;0.66. Apoptosis induced by 50 and 70 &mu;M of Chrysin was significantly greater than in untreated cells (p&lt;0.05). Cells treated with high concentration of Chrysin (90 &mu;M) showed more prominent growth inhibition and cell shrinkage compared to cells treated with the lower concentrations of Chrysin. Conclusion: The results of this study showed that Chrysin effects on AGS cell line were significantly high and dose-dependent and might be helpful in the treatment of gastric cancer. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo.</p

Effect of Epigallocatechin-3-gallate (EGCG) on cell proliferation inhibition and apoptosis induction in lymphoblastic leukemia cell line

Introduction: Acute lymphoblastic leukemia (ALL) is one of the malignant proliferations of lymphoid cells in the early stages of differentiation and accounts for &frac34; of all cases of childhood leukemia. Available treatment cannot completely treat this disease. Epigallocatechin-3-gallate (EGCG) is a polyphenolic compounds in the green tea that has demonstrated to have anticancer and antimitotic properties. The purpose of the present study was the evaluation of the effect of EGCG on the proliferation inhibition and apoptosis induction in a lymphoblastic leukemia cell line. Methods: Jurkat cell line was cultured in standard condition and in different concentrations of EGCG (0-100 micromolar) for 24, 48 and 72 hours. Cell viability was measured by MTS assay. Apoptosis induction was assessed by annexin V-FITC and flow cytometry analysis. Results: The MTS assay revealed that EGCG has decreased cell viability with a time and dose dependent manner. The level of cell apoptosis in all used concentrations of EGCG (50, 70 and 100 &mu;m) was higher than control group (71, 40 and 31 respectively vs. 8) and reached to significant level at 100 &mu;m concentration. Conclusion: The study indicated that EGCG is effective on proliferation inhibition and apoptotic induction in Jurkat lymphoblastic cell line. Therefore, the study of the mechanism of apoptosis induction could be a step of progress toward target therapy which might be considered in the future studies.</p

Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS)

Introduction: Chrysin is a natural flavonoid antioxidant which its role in tumor cell death has been reported. The aim of this study was to investigate the mechanism of Chrysin effect on proliferation and apoptosis of gastric cancer cell line (AGS). Methods: Cells were cultured and treated with different concentrations of Chrysin (90, 80, 70, 60, 50 μM) for 48 h and evaluated for cell viability. To examine the cytotoxic effect of drug in inducing apoptosis, staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) was used. The cells were treated for 48 h with different concentrations of Chrysin (50 of 90 μM) and examined for the morphological changes. The one-way analysis of variance (ANOVA) and the Excel software were used for data analysis. Results: Different concentrations of Chrysin significantly inhibited the growth and proliferation of AGS cells (p<0.05). The IC50 dose was determined to be 67.5±0.66. Apoptosis induced by 50 and 70 μM of Chrysin was significantly greater than in untreated cells (p<0.05). Cells treated with high concentration of Chrysin (90 μM) showed more prominent growth inhibition and cell shrinkage compared to cells treated with the lower concentrations of Chrysin. Conclusion: The results of this study showed that Chrysin effects on AGS cell line were significantly high and dose-dependent and might be helpful in the treatment of gastric cancer. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo

Effect of Epigallocatechin-3-gallate (EGCG) on cell proliferation inhibition and apoptosis induction in lymphoblastic leukemia cell line

Introduction: Acute lymphoblastic leukemia (ALL) is one of the malignant proliferations of lymphoid cells in the early stages of differentiation and accounts for ¾ of all cases of childhood leukemia. Available treatment cannot completely treat this disease. Epigallocatechin-3-gallate (EGCG) is a polyphenolic compounds in the green tea that has demonstrated to have anticancer and antimitotic properties. The purpose of the present study was the evaluation of the effect of EGCG on the proliferation inhibition and apoptosis induction in a lymphoblastic leukemia cell line. Methods: Jurkat cell line was cultured in standard condition and in different concentrations of EGCG (0-100 micromolar) for 24, 48 and 72 hours. Cell viability was measured by MTS assay. Apoptosis induction was assessed by annexin V-FITC and flow cytometry analysis. Results: The MTS assay revealed that EGCG has decreased cell viability with a time and dose dependent manner. The level of cell apoptosis in all used concentrations of EGCG (50, 70 and 100 μm) was higher than control group (71%, 40% and 31% respectively vs. 8%) and reached to significant level at 100 μm concentration. Conclusion: The study indicated that EGCG is effective on proliferation inhibition and apoptotic induction in Jurkat lymphoblastic cell line. Therefore, the study of the mechanism of apoptosis induction could be a step of progress toward target therapy which might be considered in the future studies