Deep learning for cancer tumor classification using transfer learning and feature concatenation

Deep convolutional neural networks (CNNs) represent one of the state-of-the-art methods for image classification in a variety of fields. Because the number of training dataset images in biomedical image classification is limited, transfer learning with CNNs is frequently applied. Breast cancer is one of most common types of cancer that causes death in women. Early detection and treatment of breast cancer are vital for improving survival rates. In this paper, we propose a deep neural network framework based on the transfer learning concept for detecting and classifying breast cancer histopathology images. In the proposed framework, we extract features from images using three pre-trained CNN architectures: VGG-16, ResNet50, and Inception-v3, and concatenate their extracted features, and then feed them into a fully connected (FC) layer to classify benign and malignant tumor cells in the histopathology images of the breast cancer. In comparison to the other CNN architectures that use a single CNN and many conventional classification methods, the proposed framework outperformed all other deep learning architectures and achieved an average accuracy of 98.76%

Astrophysical parameters of ten poorly studied open star clusters

We present here the fundamental parameters of ten open star clusters, nominated from Kronberger et al. (2006) who presented some new discovered stellar groups on the basis of 2MASS photometry and DSS visual images. Star counts and photometric parameters (radius, membership, distances, color excess, age, luminosity function, mass function, total mass, and the dynamical relaxation time) have been determined for these ten clusters for the first time. In order to calibrate our procedures, the main parameters (distance, age, and color excesses) have been re-estimated for another five clusters, which are studied by Kronberger et al. (2006) as well.Comment: 10 pages, 5 figures, 2 tables; accepted in "Research in Astronomy and Astrophysics Journal

Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study

Background: Surgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world. Methods: This international, prospective, multicentre cohort study included consecutive patients undergoing elective or emergency gastrointestinal resection within 2-week time periods at any health-care facility in any country. Countries with participating centres were stratified into high-income, middle-income, and low-income groups according to the UN's Human Development Index (HDI). Data variables from the GlobalSurg 1 study and other studies that have been found to affect the likelihood of SSI were entered into risk adjustment models. The primary outcome measure was the 30-day SSI incidence (defined by US Centers for Disease Control and Prevention criteria for superficial and deep incisional SSI). Relationships with explanatory variables were examined using Bayesian multilevel logistic regression models. This trial is registered with ClinicalTrials.gov, number NCT02662231. Findings: Between Jan 4, 2016, and July 31, 2016, 13 265 records were submitted for analysis. 12 539 patients from 343 hospitals in 66 countries were included. 7339 (58·5%) patient were from high-HDI countries (193 hospitals in 30 countries), 3918 (31·2%) patients were from middle-HDI countries (82 hospitals in 18 countries), and 1282 (10·2%) patients were from low-HDI countries (68 hospitals in 18 countries). In total, 1538 (12·3%) patients had SSI within 30 days of surgery. The incidence of SSI varied between countries with high (691 [9·4%] of 7339 patients), middle (549 [14·0%] of 3918 patients), and low (298 [23·2%] of 1282) HDI (p < 0·001). The highest SSI incidence in each HDI group was after dirty surgery (102 [17·8%] of 574 patients in high-HDI countries; 74 [31·4%] of 236 patients in middle-HDI countries; 72 [39·8%] of 181 patients in low-HDI countries). Following risk factor adjustment, patients in low-HDI countries were at greatest risk of SSI (adjusted odds ratio 1·60, 95% credible interval 1·05–2·37; p=0·030). 132 (21·6%) of 610 patients with an SSI and a microbiology culture result had an infection that was resistant to the prophylactic antibiotic used. Resistant infections were detected in 49 (16·6%) of 295 patients in high-HDI countries, in 37 (19·8%) of 187 patients in middle-HDI countries, and in 46 (35·9%) of 128 patients in low-HDI countries (p < 0·001). Interpretation: Countries with a low HDI carry a disproportionately greater burden of SSI than countries with a middle or high HDI and might have higher rates of antibiotic resistance. In view of WHO recommendations on SSI prevention that highlight the absence of high-quality interventional research, urgent, pragmatic, randomised trials based in LMICs are needed to assess measures aiming to reduce this preventable complication

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe

Global variation in anastomosis and end colostomy formation following left-sided colorectal resection

Background End colostomy rates following colorectal resection vary across institutions in high-income settings, being influenced by patient, disease, surgeon and system factors. This study aimed to assess global variation in end colostomy rates after left-sided colorectal resection. Methods This study comprised an analysis of GlobalSurg-1 and -2 international, prospective, observational cohort studies (2014, 2016), including consecutive adult patients undergoing elective or emergency left-sided colorectal resection within discrete 2-week windows. Countries were grouped into high-, middle- and low-income tertiles according to the United Nations Human Development Index (HDI). Factors associated with colostomy formation versus primary anastomosis were explored using a multilevel, multivariable logistic regression model. Results In total, 1635 patients from 242 hospitals in 57 countries undergoing left-sided colorectal resection were included: 113 (6·9 per cent) from low-HDI, 254 (15·5 per cent) from middle-HDI and 1268 (77·6 per cent) from high-HDI countries. There was a higher proportion of patients with perforated disease (57·5, 40·9 and 35·4 per cent; P < 0·001) and subsequent use of end colostomy (52·2, 24·8 and 18·9 per cent; P < 0·001) in low- compared with middle- and high-HDI settings. The association with colostomy use in low-HDI settings persisted (odds ratio (OR) 3·20, 95 per cent c.i. 1·35 to 7·57; P = 0·008) after risk adjustment for malignant disease (OR 2·34, 1·65 to 3·32; P < 0·001), emergency surgery (OR 4·08, 2·73 to 6·10; P < 0·001), time to operation at least 48 h (OR 1·99, 1·28 to 3·09; P = 0·002) and disease perforation (OR 4·00, 2·81 to 5·69; P < 0·001). Conclusion Global differences existed in the proportion of patients receiving end stomas after left-sided colorectal resection based on income, which went beyond case mix alone

Biochemical and Molecular Genetics Identification of Salicornia sp. and Sarcocornia sp. in the North Coast of Egypt

Because water scarcity anticipated to increase within the destiny in particular with growing global population and the rise in prosperity problem of the shortage of water suitable for cultivation of meals plants inside the global is growing in arid and semi-arid regions. There is the call to discover some other plant resource that doesn’t need freshwater i.e. able to grow using seawater. It is worth to note that Salicornia sp. and Sarcocornia sp. may be grown at the seawater. Currently, considered one of the most crucial issues dealing with Egypt is a way to provide food within the frame of limiting to be had soils for cultivation, limitation of water resources, especially after Ethiopian Nahda Dam and growing in population. Accordingly, the use of halophytes forage plants (Salicornia and Sarcocornia) using seawater has emerged as one in all the most exciting and intelligent research points. Therefore, a case observe was carried out in 2018 and 2019 to evaluate the nutritional status of Salicornia and Sarcocornia plants which can be grown on salty water in the North Coast of Egypt. Five samples of Salicornia and Sarcocornia amassed from Damietta Port Said coastal road and identified depends on phenotypic homes to Sarcocornia perennis (DA), Sarcocornia perennis (PS), Sarcocornia fruticosa, Salicornia europaea and Salicornia herbacea. Proximate composition analyses were carried out. It turned into obtrusive that, among dry biomass, carbohydrate has become in the most important proximate compositions in the Sarcocornia sp. and Salicornia sp. Tissues observed through ash. Molecular evaluation by SCoT techniques turned into done for Salicornia and Sarcocornia. The SCoT molecular marker techniques reach producing reproducible and dependable amplicons. Even though that the SCoT technique became higher in assessment for molecular variety and discrimination ability for all studied Salicornia and Sarcocornia.The results obtained have shown that the high nutritional value of the plant in terms of protein content, carbohydrates content and as a result, it is suitable for foo

Corticosteroids for improving outcomes in HELLP syndrome: a systematic review protocol

Objective: We will conduct an update of a systematic review and meta-analysis to assess the benefits and harms of corticosteroids vs placebo or no treatment in hemolysis, elevated liver enzymes and low platelets (HELLP) syndrome. Methods: The search will include bibliographic databases (CENTRAL, MEDLINE, CINAHL), citation indexes (Web of Science and Scopus), and clinical trial registries (ClinicalTrials.gov and the World Health Organization International Clinical Trials Registry Platform). We will search reference lists and explore the cited-by logs of relevant studies and previously published reviews. Randomized trials comparing any corticosteroid with placebo or no treatment in HELLP syndrome will be included. Two authors will assess risk of bias and extract data independently. The main outcome measure is maternal death. We will conduct common-effects meta-analysis where appropriate. We will conduct subgroup analyses by ante- versus postpartum administration and by type of corticosteroid. We will use the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach to create the Summary of Findings table. Discussion: The update is required for the development of the National Guideline for the management of severe preeclampsia. Keywords: Corticosteroids, HELLP Syndrome, Preeclampsia, Maternal morbidit

Malignant Rhabdoid Tumor of the Kidney Presenting as Intraocular Metastasis

A newborn infant presented with intraocular tumor that was clinically diagnosed as retinoblastoma and treated by exenteration of the globe. Within a few weeks however, a large abdominal mass was noted, which was found to involve the left kidney and right lobe of liver. Pathologic evaluation of the intraocular mass as well as fine-needle aspiration biopsy of the abdominal mass revealed features of malignant rhabdoid tumor. To our knowledge this is the first case of malignant rhabdoid tumor of the kidney with such an unusual clinical presentation

Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Background and Aim: Flinders Technology Associates (FTA) cards simplify sample storage, transport, and extraction by reducing cost and time for diagnosis. This study evaluated the FTA suitability for safe transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid culture form and from organ impression smears (tissues) under the same routine condition of microbiological laboratory along with detecting their nucleic acid over different storage conditions. Materials and Methods: Increase in bacterial count from 104 to 107 (colony-forming units/mL) of 78 isolates representing seven bacterial species was applied onto cards. FTA cards were grouped and inoculated by these bacteria and then stored at different conditions of 24-27°C, 4°C, and –20°C for 24 h, for 2 weeks, for 1 and 3 month storage, respectively. Bacteriological examination was done, after which bacterial DNA was identified using specific primers for each bacterial type and detected by polymerase chain reaction (PCR). Results: The total percentage of recovered bacteria from FTA cards was 66.7% at 24-27–C for 24 h, the detection limit was 100% in Gram-positive species, while it was 57.4% in Gram-negative ones. Regarding viable cell detection from organ impression smears, it was successful under the previous conditions. No live bacterial cells were observed by bacteriological isolation rather than only at 24-27°C for 24 h storage. All bacterial DNA were sufficiently confirmed by the PCR technique at different conditions. Conclusion: Overall, the FTA card method was observed to be a valid tool for nucleic acid purification for bacteria of animal origin in the form of culture or organ smears regardless of its Gram type and is used for a short time only 24 h for storage and transport of live bacteria specifically Gram-positive type. Moreover, the bacterial nucleic acid was intact after storage in –20°C for 3 months and was PCR amplifiable