Molecular Based Survey of Pathogens Associated with Respiratory Disease Outbreaks in Broiler Chickens in Accra

Respiratory diseases in poultry are caused by a host of pathogens that act either singly or in association with each other. The clinical signs elicited by some poultry respiratory pathogens are similar and may confuse diagnostic efforts. Definitive diagnoses involving the use of sensitive, robust and rapid laboratory method are important for the identification and differentiation of specific agent(s) during infection. Our study aimed at identifying the involvement of Infectious Bronchitis Virus, Newcastle Disease Virus and Mycoplasma gallisepticum (MG) in outbreaks of respiratory diseases in chickens in the Ga-East district of Greater Accra using the Polymerase Chain Reaction technique (PCR). Twenty (20) trachea swabs were obtained from four chicken flocks (5 per flock) with acute respiratory diseases. RNA and DNA were extracted from each sample individually. The S1 glycoprotein gene, MgC2 gene and fusion protein gene of IBV, MG and NDV respectively, was individually target in a single tube PCR reaction. Amplicons were resolved on a 1% agarose gel stained with Ethidium bromide and visualized with a UV trans-illuminator. Twenty percent (20%) of samples tested negative to all three pathogens. IBV and NDV as single agents were detected in 40% and 20% of the samples respectively. Twenty percent (20%) of the samples were found to be co-infected with IBV and NDV. None of the samples tested positive to MG. IBV and NDV play major roles in outbreaks of respiratory diseases in commercial broiler chickens in Ga-East district of Accra. Keywords: Respiratory pathogens, Broilers, Ghana, Polymerase Chain Reactio

Performance of nine cassava (Manihot esculanta Crantz) clones across three environments

The study was carried out to quantify the genotype × environment interaction (G × E) and to estimate the phenotypic stability by genotype genotype × environment (GGE) biplot of nine cassava clones comprising 5 hybrids, 3 parent checks and 1 improved variety. The study was planted across three different environments; Fumesua, Pokuase and Ejura representing forest, coastal savanna and forest transition zones, respectively. Genotype main effect was significant (P < 0.001) for fresh root yield and dry matter content, G × E interaction effect was significant (P < 0.001) for fresh root yield only and environment main effect was significant (P < 0.01) for only fresh root yield. The most stable clone for fresh root yield with above average performance was La02/026 (hybrid). The high genotype and low environment effects, and the relatively low interaction on dry matter content imply that evaluation and selection can be effectively done in fewer environments to select clones with high performance for the trait whiles fresh root yield requires multiple environments to identify clones with broad and specific adaptation

Genetic variability of three cassava traits across three locations in Ghana

A study was conducted to assess the extent of genetic variability, broad-sense heritability and correlations for fresh root weight, root number and top weight of five cassava genotypes across three locations in 2 years. Combined analysis of variance revealed highly significant genotypic effect for all the traits. Genotype x environment interaction was also significant for all the traits studied indicating considerable but, varying response of the genotypes to the environments. High broad-sense heritability and genetic advance as percent of the mean were observed for fresh root weight, suggesting that the trait is primarily under genetic control and that a simple recurrent phenotypic selection scheme would be rewarding. Phenotypic coefficients of variation values were larger than their corresponding genotypic coefficient of variation values for all traits. Correlations between the three traits were highly significant and positive indicating that simultaneous progress for the three traits is feasible

SSR-Based Genetic Structure Study of Seventy-Eight Cowpea (Vigna unguiculata (L.) Walp) Genotypes

Seventy-eight cowpea accessions were studied using short sequence repeats (SSR) technique. Genetic structure of these accessions was studied using three SSR polymorphic primers, SSR-6206, SSR-6218 and SSR-6219. A total of eight loci were scored for the three primers with a total of ten alleles. Bayesian clustering method grouped the cowpea genotypes into 4 sub-populations. Ancestral allele frequencies ranged between 0.128 and 0.802, while allele frequencies within sub-populations ranged from 0.001 and 0.997. Allele frequency divergence among sub-populations ranged from 0.145 to 0.406. Expected heterozygosity between individuals in the same sub-population ranged from 0.084 and 0.26, Mean genetic differentiation among sub-populations ranged from 0.374 and 0.687, with a mean geneflow ranging from 0.228 and 0.837. There was relative uniformity within the sub-populations which can be accounted for by independent random genetic drift

Effect of Gamma Irradiation on Chlorophyll Content in the Cowpea (Vigna unguiculata (L.) Walp)

The experiment was conducted to study the mutagenic effect of gamma rays on chlorophyll content at three different physiological stages in the cowpea: pre-flowering, flowering and post-flowering. Five sets of cowpea seeds were subjected to individual doses of gamma rays: 400 Gy, 450 Gy, 500 Gy, 600 Gy and 0 Gy. The seeds were sown to raise the M1 generation. The M1 generation seeds were collected and sown in the next season to raise the M2 generation. Leaf chlorophyll content was measured for M2 generation plants. Mean chlorophyll content for pre-flowering stage ranged between 38.9 ± 8.17 (control) and 64.2 ± 6.16 (400 Gy). Flowering stage mean chlorophyll content ranged from 48.3 ± 14.4 (600 Gy) to 99.4 ± 6.22 (450 Gy). Post-harvest chlorophyll mean content ranged between 13.1&nbsp;±0.98 (600 Gy) and 38.0 ±1.90 (400 Gy). There were significant differences in treatment effects for pre-flowering (P = 0.021), flowering (P = 0.005) and harvest (P = 0.000). At pre-flowering treatment, treatment 400 Gy scored a significant increase of 64 percent (P = 0.02) above the control. The optimum dose for useful induced mutation for increases in chlorophyll concentration in the cowpea was 400 Gy

A comparison of outcomes between finger and pulp replantation/revascularization in a single centre

Background: Supermicrosurgery has allowed the replantation/revascularization of the pulp, but how does this currently compare with more proximal digit replantation/revascularization? Methods: In a retrospective case study over a 5-year period at our institute, a total of 21 patients (n = 21) had either finger or pulp replantation-revascularization posttrauma. All pulp replants had a single-vessel anastomosis viz., “artery-to-artery” or “artery-to-vein” only, with venous outflow dependent on the skin-shave technique, while more proximal replants had both arterial and venous anastomoses. Age, sex, ischemic time, handedness, smoker status, and injury-replant interval were compared between the two groups, with all procedures performed by a single surgeon. The outcome parameters studied were length of hospital stay, timeline for wound healing, viability, and functional outcomes. Results: Our patients consisted of 18 men and three women, of which 14.3% were smokers and 85.7% were right-handed. There were 11 finger replantation/revascularizations (n = 11) versus 10 pulp replantation/revascularizations (n = 10). The average age of digit replantation/revascularization patients was 44.8 years compared with 26.4 years in pulp replantation/revascularization patients (Student t test, P = 0.04). Mean ischemia time in digital replants was 67 minutes versus 32.3 minutes in pulp replantation/revascularization (Student t test, P = 0.056). Digital replantation/revascularization was viable in 72% of cases versus a 90% viability in the pulp subcohort. Conclusions: In our patient cohort, pulp replantation/revascularizations produced better postoperative viability. Where supermicrosurgery expertise is available, pulp replantation/revascularization should be considered a worthwhile option when compared with digital replantation/revascularization

Genotypic and phenotypic diversity of Mycobacterium tuberculosis complex genotypes prevalent in West Africa

Findings from previous comparative genomics studies of the Mycobacterium tuberculosis complex (MTBC) suggest genomic variation among the genotypes may have phenotypic implications. We investigated the diversity in the phenotypic profiles of the main prevalent MTBC genotypes in West Africa. Thirty-six whole genome sequenced drug susceptible MTBC isolates belonging to lineages 4, 5 and 6 were included in this study. The isolates were phenotypically characterized for urease activity, tween hydrolysis, Thiophen-2-Carboxylic Acid Hydrazide (TCH) susceptibility, nitric oxide production, and growth rate in both liquid (7H9) and solid media (7H11 and Lowenstein-Jensen (L-J)). Lineage 4 isolates showed the highest growth rate in both liquid (p = 0.0003) and on solid (L-J) media supplemented with glycerol (p<0.001) or pyruvate (p = 0.005). L6 isolates optimally utilized pyruvate compared to glycerol (p<0.001), whereas L5 isolates grew similarly on both media (p = 0.05). Lineage 4 isolates showed the lowest average time to positivity (TTP) (p = 0.01; Average TTP: L4 = 15days, L5 = 16.7days, L6 = 29.7days) and the highest logCFU/mL (p = 0.04; average logCFU/mL L4 = 5.9, L5 = 5.0, L6 = 4.4) on 7H11 supplemented with glycerol, but there was no significant difference in growth on 7H11 supplemented with pyruvate (p = 0.23). The highest release of nitrite was recorded for L5 isolates, followed by L4 and L6 isolates. However, the reverse was observed in the urease activity for the lineages. All isolates tested were resistant to TCH except for one L6 isolate. Comparative genomic analyses revealed several mutations that might explain the diverse phenotypic profiles of these isolates. Our findings showed significant phenotypic diversity among the MTBC lineages used for this study

Genomic epidemiological analysis identifies high relapse among individuals with recurring tuberculosis and provides evidence of household recent TB transmission in Ghana

OBJECTIVE: We investigated the cause of recurring tuberculosis (rcTB) among pulmonary TB participants recruited from a prospective population-based study conducted between July 2012 and December 2015. METHODS: Mycobacterium tuberculosis complex isolates obtained from rcTB cases were characterized by standard mycobacterial genotyping tools in addition to whole genome sequencing, followed by phylogenetic analysis to assess strain relatedness. RESULTS: A greater proportion (58.3%, 21/36) of individuals with rcTB episodes had TB recurrence within 12 months post treatment. Only 19.4% (7/36) of participants with rcTB harbored a strain with isoniazid (INH) resistance at baseline of which 29% (2/7) were additionally resistant to rifampicin. However, 27.8% (10/36) harbored an INH resistant strain upon recurring of which 40% (4/10) were MDR-TB strains. Recurrent TB was attributed to relapse (same strain) in 75.0% (27/36) of participants with 25.0% (9/36) attributed to re-infection. CONCLUSION: Our findings indicate that unresolved previous infection due to inadequate treatment may be the major cause of rcTB

“I am Italian in the world”: A mobile student’s story of language learning and ideological becoming

This article theorises the relationship between language and intercultural learning from a Bakhtinian dialogic perspective, based on the language learning story of Federica, a mobile student in UK higher education (HE). I first outline the context of UK HE and its internationalisation agenda, discussing how research in this field has conceptualised language, intercultural communication (IC), and international students in terms of a totalising boundary between self and other. I link this to current concerns in IC regarding the philosophical underpinnings of the field, specifically the aporia created as a result of the totalising self/other relation in prevailing IC discourse (MacDonald & O’Regan, 2013). I then present a means of addressing this aporia through a Bakhtinian theorisation of the relationship between language and intercultural learning. This theorisation offers a relational perspective on the self and the other in which intercultural learning is a process of ideological becoming (Bakhtin, 1981) with the other, enacted in, with and through language, as illustrated in Federica’s story of learning English. The article concludes with a call for language and communicative practices to be placed at the heart of HE internationalisation agendas and for HE practitioners to recognise shared responsibility for intercultural communication