Adult social isolation leads to anxiety and spatial memory impairment: Brain activity pattern of COx and c-Fos

Social isolation during adulthood is a frequent problem that leads to a large variety of adverse emotional and cognitive effects. However, most of the social isolation rodent procedures begin the separation early post-weaning. This work explores locomotor activity, anxiety-like behaviour, and spatial working memory after twelve weeks of adult social isolation. In order to study the functional contribution of selected brain areas following a working memory task, we assessed neuronal metabolic activity through quantitative cytochrome oxidase histochemistry and c-Fos immunohistochemistry. Behaviourally, we found that isolated animals (IS) showed anxiety-like behaviour and worse working memory than controls, whereas motor functions were preserved. Moreover, IS rats showed lower levels of learning-related c-Fos immunoreactivity, compared to controls, in the medial prefrontal cortex (mPFC), ventral tegmental area (VTA), and nucleus accumbens shell. In addition, the IS group showed lower neuronal metabolic activity in the mPFC, VTA, and CA1 subfield of the hippocampus. These results indicate that twelve weeks of social isolation in adult rats leads to different behavioural and brain alterations, and they highlight the importance of social support, not only in development, but also in adulthood

Study of sperm parameters in the retuertas horse

Geographical concentration and scarce census of the Retuertas horse force to design a complete conservation plan. One of the used tools as a mean of ex situ conservation was the creation of a germplasm bank. Previously, a study of the se-minal quality and its aptitude for freezing was developed. Results were: volume 23,25±12,72 ml; concentration 335,83±135,60x106 spermatozoa/ml; basal motility 3,83±0,98 (1-5) and progressive motility 60,42±15,42 (0-100%); all of them shown significant differences among stallions.La concentración geográfica y el reducido censo del Caballo de las Retuertas obligaron a diseñar un Plan de Conservación integral. Una de las herramientaspara la conservación ex situ fue la creación de un banco de germoplasma. Previamente, se estudió la calidad seminal y aptitud para congelación. Los resultados obtenidos fueron: volumen 23,25±12,72 ml, concentración 335,83±135,60x106 espermatozoides/ml; motilidad masal 3,83±0,98 (1-5) y motilidad progresiva 60,42±15,42 (0-100%), mostrando todas diferencias significativas en relación al semental

Velocidades radiales de galaxias australes

Se informa sobre el estado de avance del programa de velocidades radiales de galaxias con el Espectrógrafo Nebular de Page.Asociación Argentina de Astronomí

Curvas de rotación en galaxias australes

Se dan los resultados del análisis de las curvas de rotación de 9 galaxias obtenidas con el Espectrógrafo Rápido Page y tubo de imágenes en Bosque Alegre. Se estiman masas y densidades.Asociación Argentina de Astronomí

Good character at college: the combined role of second-order character strength factors and phronesis motivation in undergraduate academic outcomes

A renewed interest in the study of character and virtue has recently emerged in the fields of Education and Psychology. The latest research has confirmed the association between virtuous consistent behaviours and academic positive outcomes. However, the motivational dimension of character (the intentions underlying the patterns of observed behaviours) has received little attention. This research aims to extend the knowledge on this topic by examining the predictive relationships between the behavioural and motivational dimensions of character, with reference to academic engagement, career self-doubt and performance of Spanish university students. A total of 183 under- graduates aged 18–30 (142 of whom were women) from the north of Spain completed specific parts of self-report questionnaires, including the Values in Action VIA-72, a Spanish translated and validated version of the Moral Self-Relevance Measure MSR, and the Utrecht Work Engagement Student Scale UWES-S9. The collected data were analysed using Structural Equation Modelling. The behavioural dimension of character (character strength factors of caring, self-control and inquisitiveness) showed positive associations with academic engagement and performance. The motivational dimension of character (phronesis motivation), was negatively related to career self-doubt. For the first time, the present study has provided support for the contribution of both dimensions of character to undergraduate academic outcomes

Reaction-diffusion processes in zero transverse dimensions as toy models for high-energy QCD

We examine numerically different zero-dimensional reaction-diffusion processes as candidate toy models for high-energy QCD evolution. Of the models examined -- Reggeon Field Theory, Directed Percolation and Reversible Processes -- only the latter shows the behaviour commonly expected, namely an increase of the scattering amplitude with increasing rapidity. Further, we find that increasing recombination terms, quantum loops and the heuristic inclusion of a running of the couplings, generically slow down the evolution.Comment: 17 pages, 7 figure

Pulsating flow and convective heat transfer in a cavity with inlet and outlet sections

This paper deals with the study of 2-D, laminar, pulsating flow inside a heated rectangular cavity with different aspect ratios. The cooling liquid (water with temperature dependent viscosity and thermal conductivity) comes and leaves the cavity via inlet and outlet ports. The flow topology is characterised by the large recirculation regions that exist at inner corners of the cavity. These low velocity regions cause the heat transfer to be small when compared, for instance, to that of a straight channel. We study the effect that a prescribed pulsation at the inlet port has on the cavity heat transfer. This pulsating boundary condition, of the unsteady Poiseuille type, is described by its frequency and the amplitude of the pressure gradient. The time averaged Reynolds number of the flow, based on the hydraulic diameter of the inlet channel, is 100 and we consider that the dimensionless pulsation frequency (Strouhal number) varies in the range from 0.0 to 0.4. We show that the prescribed pulsation enhances heat transfer in the cavity and that the mechanism that causes this enhancement appears to be the periodic change in the recirculation flow pattern generated by the pulsation. Regarding the quantitative extent of heat transfer recovery, we find that appropriate selection of the pulsation parameters allows for the cavity to behave like a straight channel that is the configuration with the highest Nusselt number

Mechanical Identities of RNA and DNA Double Helices Unveiled at the Single-Molecule Level

[EN] Double-stranded (ds) RNA is the genetic material of a variety of viruses and has been recently recognized as a relevant molecule in cells for its regulatory role. Despite that the elastic response of dsDNA has been thoroughly characterized in recent years in single-molecule stretching experiments, an equivalent study with dsRNA is still lacking. Here, we have engineered long dsRNA molecules for their individual characterization contrasting information with dsDNA molecules of the same sequence. It is known that dsRNA is an A-form molecule unlike dsDNA, which exhibits B-form in physiological conditions. These structural types are distinguished at the single-molecule level with atomic force microscopy (AFM) and are the basis to understand their different elastic response. Force¿extension curves of dsRNA with optical and magnetic tweezers manifest two main regimes of elasticity, an entropic regime whose end is marked by the A-form contour- length and an intrinsic regime that ends in a low-cooperative overstretching transition in which the molecule extends to 1.7 times its A-form contour-length. DsRNA does not switch between the A and B conformations in the presence of force. Finally, dsRNA presents both a lower stretch modulus and overstretching transition force than dsDNA, whereas the electrostatic and intrinsic contributions to the persistence length are larger.This work was supported by grants from the Spanish Ministry of Science and Innovation (BFU2011-29038 and BFU2010-15703) and the Comunidad de Madrid (S2009/MAT/1507). IRA.-G. acknowledges a Ramon y Cajal contract from the Spanish Ministry of Science and Innovation (RYC-2007-01765). Work in the F.M.-H. laboratory was supported by a Starting Grant from the European Research Council (no. 206117) and a grant from the Spanish Ministry of Science and Innovation (FIS2011-24638). We thank M. S. Dillingham for kindly providing the pSP73-JY0 plasmid, M. Menendez for access to a spectropolarimeter, A. Monserrate for polylysine-AFM control experiments, and B. Ibarra for fruitful discussions.Herrero-Galán, E.; Fuentes-Perez. M.E.; Carrasco, C.; Valpuesta, J.; Carrascosa, J.; Moreno-Herrero, F.; Arias-Gonzalez, JR. (2013). Mechanical Identities of RNA and DNA Double Helices Unveiled at the Single-Molecule Level. Journal of the American Chemical Society. 135(1):122-131. https://doi.org/10.1021/ja3054755S122131135