Antimalarial, anticancer, antimicrobial activities and chemical constituents of essential oil from the aerial parts of Cyperus kyllingia Endl.

The chemical constituents of the essential oil from Cyperus kyllingia Endl. were analyzed by a GC, GC-MS. Twenty-three compounds were identified, accounting for 93.75% of the total oil that consisted mainly of oxygenated sesquiterpenes (53.52%), particularly sesquiterpene hydrocarbons (38.97%), and carboxylic acid (1.26%). The most representative compounds were α-cadinol (19.32%), caryophyllene oxide (12.17%), α-muurolol (11.58%), α-humulene (9.85%), and α-atlantone (6.07%). The oil showed significant activities against Plasmodium falcipalum (K1, multi drug resistant strain) and NCI-H187 (Small Cell Lung Cancer) with the IC50 values of 7.52 and 7.72 μg/mL, respectively. The oil exhibited highly active against Staphylococcus aureus ATCC25923 and moderately active against Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27553, Aspergillus flavus and Candida albicans

Izolacija bioaktivnih flavonoida iz biljke Jacaranda obtusifolia H. B. K. ssp. rhombifolia (G. F. W. Meijer) Gentry

The paper describes the bioassay-guided isolation, structure elucidation and anticancer evaluation of five flavonoids ()-liquiritigenin (1), ()-neoliquiritin (2), isoliquiritigenin (3), isoliquiritin (4) and formononetin (5) from the twigs of Jacaranda obtusifolia H. B. K. ssp. rhombifolia (G. F. W. Meijer) Gentry. The structures were elucidated based on 1H, 13C NMR, comprehensive 2D NMR, MS analyses and comparison with previously reported spectral data. Compounds 1 and 3 were demonstrated to be inhibitory in vitro against NCI-H187 (small cell lung cancer) with IC50 values of 30.1 and 16.6 µg mL1, respectively. The isolates were non-cytotoxic to Vero cells (African green monkey kidney).U radu je opisana izolacija, određivanje strukture i antitumorsko djelovanje pet flavonoida: ()-likviritigenina (1), ()-neolikviritina (2), izolikviritigenina (3), izolikviritina (4) i formononetina (5) iz plodova biljke Jacaranda obtusifolia H. B. K. ssp. rhombifolia (G. F. W. Meijer) Gentry. Strukture su određene na temelju 1H, 13C NMR, 2D NMR, MS i usporedbom s ranije objavljenim spektroskopskim podacima. Spojevi 1 i 3 imaju inhibitorni učinak in vitro na tumorsku staničnu liniju raka pluća NCI-H187 (IC50 vrijednost 30,1, odnosno 16,6 µg mL1). Izolirani flavonoidi nisu citotoksični za Vero stanice (bubrežne stanice afričkog zelenog majmuna)

A new isoflavone from Jacaranda obtusifolia H.B.K. ssp. rhombifolia (G.F.W. Meijer) gentry

A new isoflavone (1), along wilh daidzein (2), and genistin (3) were isolated from the Jacaranda oblusifolia twig extract. Their structures were elucidated by NMR and MS spectroscopy. Compound 1 possessed significant anticancer activity against KB and NC1-H187 cell lines with the IC values of 2.52 and 7.47 ng mL, compound 2 and 3 exhibited anticancer activity against KB and NC1-HI87 cell lines wilh the IC values of 5.73, 8.14, 2.83 and 27.30 μg mL, respectively. Compound 2 also showed anticancer activity against MCF-7 cell line with an IC value of 28.17 μg mL. The isolates showed non-cytotoxic against Vero cells

Anticancer and antibacterial activities of the isolated compounds from Solanum spirale Roxb. leaves

The isolations of active anticancer compounds from Solanum spirale Roxb., collected from Phayao Province, Thailand are rarely reported. The anticancer activities of hexane and methanol extracts of the leaves were investigated. The chloroform extract possessed anticancer activities against KB-Oral cavity cancer, MCF-7 breast cancer cell and NCI-H187-Small cell lung cancer with the IC50 values of 42.73, 17.90, and 36.74 μg/ mL, respectively. But the hexane extract did not inhibit significant anticancer activity against three cancer cell lines. Lupeol, protocatechuic acid and trans-cinnamic acid have been isolated from the chloroform extract of this medicinal plants. Their structures were characterized on the basis of spectroscopic analysis. The anticancer activities of the isolated compounds were also evaluated in vitro against three cancer cell lines. Only lupeol compound inhibited significant anticancer activity against KB-Oral cavity cancer with the IC50- value of 26.73 μg/mL, whereas protocatechuic acid and trans-cinnamic acid did not exhibit anticancer activity. The hexane, chloroform extracts and the isolated compounds did not show cytotoxic activity against the African green monkey kidney cell line. The hexane and chloroform extracts also inhibited antibacterial activity against Escherichia coli and Staphylococcus aureus with the MIC values were in the range between 375-1500 μg/mL. All isolated compounds showed equal antibacterial activity against E. coli with the MIC of 250 μg/mL, and trans-cinnamic acid showed better antibacterial activity against S. aureus with the MIC of 250 μg/mL than lupeol and protocatechuic acid. This is the first report decribes the isolation and structure elucidation of lupeol, protocatechuic acid and trans-cinnamic acid together with their anticancer, cytotoxic and antibacterial activities from this medicinal plant