Manhattan plot of the GWAS meta-analysis results of the three Finnish cohorts. The horizontal line represents the genome-wide significance threshold (p<5E-08).

<p>Manhattan plot of the GWAS meta-analysis results of the three Finnish cohorts. The horizontal line represents the genome-wide significance threshold (p<5E-08).</p

QQ plot of the GWAS meta-analysis results of the three Finnish cohorts (λ = 1.027).

<p>QQ plot of the GWAS meta-analysis results of the three Finnish cohorts (λ = 1.027).</p

Detailed results for the most interesting signals on 19q13.2.

<p>MAF, minor allele frequency; GWAS, genome-wide association study; SD, standard deviation; EUR, 1000Genomes reference panel of individuals of European descent; N/A, not available;</p><p>¹<i>beta</i> reported for the coded allele;</p><p>² MAF calculated among 19857 FINRISK individuals;</p><p>³ MAF reported for the EUR population at the Ensembl database;</p><p>⁴ change in NMR is estimated by multiplying SD of NMR (SD = 0.23 in the combined meta-analysis sample) by the effect size of the minor allele;</p><p>⁵ rs113288603 showed no association in the GWAS but was identified as an independent signal in analyses conditioned on the top-SNP (rs56113850);</p><p>⁶ identified as an independent SNP only in the FINRISK2007 GWAS sample;</p><p>⁷ rs12461964 was not an independent signal in FinnTwin or YFS, thus variance explained was not estimated;</p><p>⁸ rs1801272;</p><p>⁹ not included in the GWAS due to MAF<0.01;</p><p>¹⁰ rs28399433;</p><p>¹¹ rs28399435;</p><p>¹² rs1809810;</p><p>¹³ rs6413474.</p><p>First four rows represent the independent signals in the ranking order from the meta-analysis of conditional analyses. Last five rows represent known <i>CYP2A6</i> alleles present in our data. Distribution of NMR and rank transformed NMR among the different genotype groups of these nine SNPs in the YFS sample are presented in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005498#pgen.1005498.s006" target="_blank">S6 Fig</a>.</p

Phenotype and covariate proportions (%) and means (min-max, SD) in the FinnTwin, Young Finns Study (YFS), and FINRISK2007 samples.

<p>Phenotype and covariate proportions (%) and means (min-max, SD) in the FinnTwin, Young Finns Study (YFS), and FINRISK2007 samples.</p

Logistic regressions on the associations of the <i>DRD3</i> variant rs2399496 and nicotine dependence (ND) with major depressive disorder (MDD): Models combining the ND (no/yes) and rs2399496 (TT = 0, TA = 1, or AA = 2 minor alleles) status.

a<p>Adjusted for sex, age, and alcohol abuse.</p

Basic characteristics of the study samples used in (A) genetic association analyses (N = 1428) and (B) logistic regressions (N = 1296).

a<p>, 74 with unconfirmed zygosity; ^b, 34 with unconfirmed zygosity; ^c 44 with unconfirmed zygosity; ^d 23 with unconfirmed zygosity: *, Conditioned for regular smoking.</p><p>MZ, monozygotic; DZ, dizygotic; CPD, cigarettes per day; DSM-IV, Diagnostic and Statistical Manual of Mental Disorders, 4^th edition (APA 1994); MDD, major depressive disorder; ND, nicotine dependence.</p

Association analyses results (p-values) for <i>DRD3</i> SNPs.

<p>DSM-IV, Diagnostic and Statistical Manual of Mental Disorders, 4^th edition (APA 1994); MDD, major depressive disorder; ND, nicotine dependence.</p>a<p>, significant p-value (study-specific threshold p<0.00042).</p>b<p>, suggestive p-value (study-specific threshold 0.00042</p