Effect of allopurinol on rat testicles morphology, submitted to ischemia for spermatic cord torsion, followed by reperfusion

A torção do cordão espermático (TCE) é uma afecção bastante frequente na cirurgia urológica pediátrica. O fenômeno de isquemia-reperfusão e os métodos antioxidantes têm sido amplamente estudados, porém com resultados bastante conflitantes. Estudamos o efeito do alopurinol no fenômeno de isquemia-reperfusão em testículos de ratos que foram submetidos à TCE. Foram utilizados 80 ratos Wístar, adultos, distribuídos em 4 grupos: controle sem alopurínol, controle com alopurínol, torção do cordão espermático sem alopurinol e torção do cordão espermático com alopurinol. Os testículos foram retirados após 60 dias do ato operatório e processados para exame histológico à microscopia óptica, onde os tábulos seminíferos e as células espermatogênicas foram objeto de estudo. Houve significativa aúofia dos testículos cujo cordão foi torcido e o alopurinol não progeu as gônadas dos efeitos da isquemia reperfusão.BV UNIFESP: Teses e dissertaçõe

Morphologic and biomechanic study of abdominal wall repair with prosthesis in grownt and development organisms

Realizou-se estudo morfologico e biomecanico do reparo da parede abdominal em 90 ratos Wistar com telas de polipropileno, politetrafluoroetileno expandido e politetrafluoroetileno expandido tratado com agente antimicrobiano e poliglactina 910. No grupo controle o defeito na parede abdominal nao recebeu tela, fechando-se apenas a pele. Os grupos III, IV, V e VI receberam telas de Polipropileno, poliglactina 910, Politetrafluoroetileno expandido e Politetrafluoreetileno expandido tratado com agente antimicrobiano, respectivamente. Apos 90 dias, realizou-se analise macroscopica com observacao de eventracoes, aderencias e sinais inflamamatorios; analise biomecanica com quantificacao da resistencia a tracao; e analise histologica da integracao da tela com quantificacao de colageno e macrofagos. Os resultados foram tratados estatisticamente por testes de analise de variancia e quiquadrado. O conjunto de resultados mostrou retardo de cicatrizacao e menor resistencia a tracao nos animais do Grupo IV; nao houve diferenca significante na incorporacao aos tecidos, entre os grupos estudadosBV UNIFESP: Teses e dissertaçõe

The use of polidioxanone and poliamide sutures in rabbit's tenorraphy

OBJECTIVE: comparative study between the mechanical properties of the tenorraphies with poliamida (nylon) and polidioxanone (PDS). METHODS: 56 adult, male, New Zealand rabbits were used, distributed in two groups and four sub groups: GIA-tenorraphies with polidioxanone and evaluation after two weeks; GIB-tenorraphies with polidioxanone and evaluation after four weeks; GIIA-tenorraphies with nylon and evaluation after two weeks; GIIB-tenorraphies with nylon and evaluation after four weeks. Traction tests were done in tenorraphies of the rabbits. RESULTS: after fifteen days nylon presented as results of the mechanical tests, superior values that the one of PDS for maximum load, rigidity coefficient, deformation, deformation in the proportionality limit, load in the proportionality limit, that were the variable analysed. CONCLUSION: after thirty days there was not significant difference between nylon and PDS.OBJETIVO: estabelecer comparação entre as propriedades mecânicas das tenorrafias realizadas com poliamida (náilon) e polidioxanona (PDS). MÉTODOS: Foram utilizados 56 coelhos adultos, machos, Nova Zelândia, distribuídos em dois grupos e quatro subgrupos: GIA - tenorrafias com polidioxanona e avaliação após duas semanas; GIB - tenorrafias com polidioxanona e avaliação após quatro semanas; GIIA - tenorrafias com náilon e avaliação após duas semanas; GIIB - tenorrafias com náilon e avaliação após quatro semanas. Foram realizados ensaios de tração nas tenorrafias dos tendões dos coelhos. RESULTADOS: na avaliação aos quinze dias de pós-operatório, o náilon apresentou como resultados dos ensaios mecânicos, valores superiores que os do PDS para carga máxima, coeficiente de rigidez, deformação, deformação no limite de proporcionalidade, carga no limite de proporcionalidade, que foram as variáveis analisadas. CONCLUSÃO: aos trinta dias de pós-operatório não houve diferença significante entre o uso do náilon e o PDS.UFMSUNIFESP-EPMUNIFESP-EPM Departamento de CirurgiaUNIFESP, EPM, Depto. de CirurgiaSciEL

Mesenchymal Stromal Cell Therapy Reverses Detrusor Hypoactivity in a Chronic Kidney Patient

Detrusor hypoactivity (DH) is characterized by low detrusor pressure or a short contraction associated with low urinary flow. This condition can progress to chronic renal failure (CRF) and result in the need for dialysis. The present case report demonstrates that a patient diagnosed with DH and CRF who received two transplants with 2 × 106 autologous mesenchymal stromal cells at an interval of 30 days recovered the contractile strength of the bladder and normalized his renal function. The patient had a score of 19 on the ICIQ-SF before cell therapy, and that score was reduced to 1 after transplantation. These results demonstrate that there was an improvement in his voiding function, urinary stream and urine volume as evaluated by urofluxometry. In addition, a urodynamic study carried out after treatment showed an increase in the maximum flow from 2 mL/s to 23 mL/s, the detrusor pressure in the maximum flow from 21 cm H2O to 46 cm H2O and a BCI that went from 31 to 161, characterizing good detrusor contraction. Thus, in the present case, the transplantation of autologous mesenchymal stromal cells proved to be a viable therapeutic option to allow the patient to recover the contractile strength of the bladder, and reversed the CRF

Mesenchymal stem cell therapy promotes the improvement and recovery of renal function in a preclinical model

Abstract Acute renal failure (ARF) is an extremely important public health issue in need of novel therapies. The present study aimed to evaluate the capacity of mesenchymal stem cell (MSC) therapy to promote the improvement and recovery of renal function in a preclinical model. Wistar rats were used as the experimental model, and our results show that cisplatin (5mg/kg) can efficiently induce ARF, as measured by changes in biochemical (urea and creatinine) and histological parameters. MSC therapy performed 24h after the administration of chemotherapy resulted in normalized plasma urea and creatinine levels 30 and 45d after the onset of kidney disease. Furthermore, MSC therapy significantly reduced histological changes (intratubular cast formation in protein overload nephropathy and tubular hydropic degeneration) in this ARF model. Thus, considering that current therapies for ARF are merely palliative and that MSC therapy can promote the improvement and recovery of renal function in this model system, we suggest that innovative/alternative therapies involving MSCs should be considered for clinical studies in humans to treat ARF

Fator de crescimento nervoso em cola de fibrina no reparo término-lateral de nervos em ratos

Purpose: To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. Methods: Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. Results: All the analyzed variables did not show any differences among the four operated groups (p>0.05), which were statistically superior to group B (p0.05). Conclusion: The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating