Determinants of occupational allergic respiratory disease and asthma in spice mill workers

Includes abstract.Includes bibliographical references (leaves 94-100).The aim of this study was to determine the prevalence of occupational allergy and asthma associated with airborne spice and to determine the host and environmental risk factors associated with allergic respiratory disease among spice mill workers. A cross-sectional epidemiological study of 150 currently employed workers in a spice mill was conducted. Environmental exposure assessment entailed the collection of 62 full-shift airborne personal samples on randomly selected individuals employed in various departments of the spice mill using the NIOSH occupational exposure sampling strategy manual. The samples were analysed for inhalable particulate mass, specific spice dust allergens (garlic) and endotoxin using ELISA inhibition (antibodies from sensitised subjects) and chromogenic LAL assays

Meteorologically estimated exposure but not distance predicts asthma symptoms in schoolchildren in the environs of a petrochemical refinery: a cross-sectional study

<p>Abstract</p> <p>Background</p> <p>Community concern about asthma prompted an epidemiological study of children living near a petrochemical refinery in Cape Town, South Africa. Because of resource constraints and the complexity of refinery emissions, neither direct environmental measurements nor modelling of airborne pollutants was possible. Instead a meteorologically derived exposure metric was calculated with the refinery as the putative point source. The study aimed to determine whether (1) asthma symptom prevalences were elevated compared to comparable areas in Cape Town and (2) whether there was an association between asthma symptom prevalences and the derived exposure metric.</p> <p>Methods</p> <p>A cross-sectional study was carried out of all consenting school children aged 11 to 14 years attending schools in a defined area, utilizing the International Study of Asthma and Allergy in Childhood (ISAAC) written and video questionnaires. Information was collected on potential confounders, e.g. parental history of atopic disease, active and passive smoking by the participant, birth order, number of children in the home and distance from a major road. The exposure metric combined residential distance of each child from the refinery with a wind vector in the form of wind speed, wind direction and proportion of the year blown.</p> <p>Results</p> <p>A total of 2,361 children from 17 schools met the criteria for inclusion. In multivariate analysis, meteorologically estimated exposure (MEE), but not simple distance from the refinery, was positively associated with having to take an inhaler to school [odds ratio per interquartile range (OR) 1.22, 95% confidence interval (CI) 1.06-1.40], and with a number of video elicited asthma symptoms, including recent waking with wheezing (OR 1.33, 95% CI 1.06-1.66) and frequent wheezing at rest (OR 1.27, 95% CI 1.05 - 1.54). Symptom prevalences were higher than in other areas of the city, with frequent waking with wheezing being in great excess (OR 8.92, 95% CI 4.79-16.63).</p> <p>Conclusion</p> <p>The results support the hypothesis of an increased prevalence of asthma symptoms among children in the area as a result of refinery emissions and provide a substantive basis for community concern. The methodology also provides a low cost means of testing hypotheses about point source pollutant effects on surrounding populations of children.</p

Mitochondrial DNA Polymorphism A4917G Is Independently Associated with Age-Related Macular Degeneration

The objective of this study was to determine if MTND2*LHON4917G (4917G), a specific non-synonymous polymorphism in the mitochondrial genome previously associated with neurodegenerative phenotypes, is associated with increased risk for age-related macular degeneration (AMD). A preliminary study of 393 individuals (293 cases and 100 controls) ascertained at Vanderbilt revealed an increased occurrence of 4917G in cases compared to controls (15.4% vs.9.0%, p = 0.11). Since there was a significant age difference between cases and controls in this initial analysis, we extended the study by selecting Caucasian pairs matched at the exact age at examination. From the 1547 individuals in the Vanderbilt/Duke AMD population association study (including 157 in the preliminary study), we were able to match 560 (280 cases and 280 unaffected) on exact age at examination. This study population was genotyped for 4917G plus specific AMD-associated nuclear genome polymorphisms in CFH, LOC387715 and ApoE. Following adjustment for the listed nuclear genome polymorphisms, 4917G independently predicts the presence of AMD (OR = 2.16, 95%CI 1.20–3.91, p = 0.01). In conclusion, a specific mitochondrial polymorphism previously implicated in other neurodegenerative phenotypes (4917G) appears to convey risk for AMD independent of recently discovered nuclear DNA polymorphisms

Work-related allergy and asthma in spice mill workers – the impact of processing dried spices on IgE reactivity patterns

Background: Three spice mill workers developed work-related allergy and asthma after prolonged exposure to high levels (>10 mg/m3) of inhalable spice dust. Patterns of sensitization to a variety of spices and putative allergens were identified. Methods: Work-related allergy and asthma were assessed on history, clinical evaluation, pulmonary function and fractional exhaled nitric oxide. Specific IgE reactivity to a range of common inhalant, food and spice allergens was evaluated using ImmunoCAP and allergen microarray. The presence of non-IgE-mediated reactions was determined by basophil stimulation (CAST-ELISA). Specific allergens were identified by immunoblotting to extracts of raw and dried processed garlic, onion and chili pepper. Results: Asthma was confirmed in all 3 subjects, with work-related patterns prominent in worker 1 and 3. Sensitization to multiple spices and pollen was observed in both atopic workers 1 and 2, whereas garlic and chili pepper sensitization featured in all 3 workers. Microarray analysis demonstrated prominent profilin reactivity in atopic worker 2. Immunoblotting demonstrated a 50-kDa cross-reactive allergen in garlic and onion, and allergens of approximately 40 and 52 kDa in chili pepper. Dry powdered garlic and onion demonstrated greater IgE binding. Conclusions: This study demonstrated IgE reactivity to multiple spice allergens in workers exposed to high levels of inhalable spice dust. Processed garlic and onion powder demonstrated stronger IgE reactivity than the raw plant. Atopy and polysensitization to various plant profilins, suggesting pollen-food syndrome, represent additional risk factors for sensitizer-induced work-related asthma in spice mill workers

Work-related allergic respiratory disease and asthma in spice mill workers is associated with inhalant chili pepper and garlic exposures

Objective The aim of the study was to determine the prevalence and risk factors for allergic respiratory disease in spice mill workers.\ud \ud Methods A cross-sectional study of 150 workers used European Community Respiratory Health Survey questionnaires, Phadiatop, serum specific IgE (garlic, chili pepper), spirometry and fractional exhaled nitric oxide (FeNO). Personal air samples (n=62) collected from eight-hour shifts were analysed for inhalable particulate mass. Novel immunological assays quantified airborne garlic and chili pepper allergen concentrations.\ud \ud Results Mean dust particulate mass (geometric mean (GM)=2.06 mg/m³), chili pepper (GM=0.44 µg/m³) and garlic allergen (GM=0.24 µg/m³) were highest in blending and were highly correlated. Workers' mean age was 33 years, 71% were men, 46% current smokers and 45% atopic. Spice-dust-related asthma-like symptoms (17%) were common, as was garlic sensitisation (19%), with 13% being monosensitised and 6% cosensitised to chili pepper. Airflow reversibility and FeNO>50 ppb was present in 4% and 8% of workers respectively. Spice-dust-related ocular-nasal (OR 2.40, CI 1.09 to 5.27) and asthma-like (OR 4.15, CI 1.09 to 15.72) symptoms were strongly associated with airborne garlic in the highly exposed (>0.235 µg/m³) workers. Workers monosensitised to garlic were more likely to be exposed to higher airborne chili pepper (>0.92 µg/m³) (OR 11.52, CI 1.17 to 113.11) than garlic allergens (OR 5.08, CI 1.17 to 22.08) in this mill. Probable asthma was also more strongly associated with chili pepper than with garlic sensitisation.\ud \ud Conclusions Exposure to inhalable spice dust (GM >2.06 mg/m³) containing garlic (GM>0.24 µg/m³) and chili pepper (GM >0.44 µg/m³) allergens increase the risk of allergic respiratory disease and asthma

VLBI20-30: a scientific roadmap for the next decade -- The future of the European VLBI Network

This white paper describes the science case for Very Long Baseline Interferometry (VLBI) and provides suggestions towards upgrade paths for the European VLBI Network (EVN). The EVN is a distributed long-baseline radio interferometric array, that operates at the very forefront of astronomical research. Recent results, together with the new science possibilities outlined in this vision document, demonstrate the EVN's potential to generate new and exciting results that will transform our view of the cosmos. Together with e-MERLIN, the EVN provides a range of baseline lengths that permit unique studies of faint radio sources to be made over a wide range of spatial scales. The science cases are reviewed in six chapters that cover the following broad areas: cosmology, galaxy formation and evolution, innermost regions of active galactic nuclei, explosive phenomena and transients, stars and stellar masers in the Milky Way, celestial reference frames and space applications. The document concludes with identifying the synergies with other radio, as well as multi-band/multi-messenger instruments, and provide the recommendations for future improvements. The appendices briefly describe other radio VLBI arrays, the technological framework for EVN developments, and a selection of spectral lines of astrophysical interest below 100 GHz. The document includes a glossary for non-specialists, and a list of acronyms at the end

VLBI20-30: a scientific roadmap for the next decade -- The future of the European VLBI Network

This white paper describes the science case for Very Long Baseline Interferometry (VLBI) and provides suggestions towards upgrade paths for the European VLBI Network (EVN). The EVN is a distributed long-baseline radio interferometric array, that operates at the very forefront of astronomical research. Recent results, together with the new science possibilities outlined in this vision document, demonstrate the EVN's potential to generate new and exciting results that will transform our view of the cosmos. Together with e-MERLIN, the EVN provides a range of baseline lengths that permit unique studies of faint radio sources to be made over a wide range of spatial scales. The science cases are reviewed in six chapters that cover the following broad areas: cosmology, galaxy formation and evolution, innermost regions of active galactic nuclei, explosive phenomena and transients, stars and stellar masers in the Milky Way, celestial reference frames and space applications. The document concludes with identifying the synergies with other radio, as well as multi-band/multi-messenger instruments, and provide the recommendations for future improvements. The appendices briefly describe other radio VLBI arrays, the technological framework for EVN developments, and a selection of spectral lines of astrophysical interest below 100 GHz. The document includes a glossary for non-specialists, and a list of acronyms at the end

Large-scale meta-analysis of genome-wide association data identifies six new risk loci for Parkinson's disease

We conducted a meta-analysis of Parkinson's disease genome-wide association studies using a common set of 7,893,274 variants across 13,708 cases and 95,282 controls. Twenty-six loci were identified as having genome-wide significant association; these and 6 additional previously reported loci were then tested in an independent set of 5,353 cases and 5,551 controls. Of the 32 tested SNPs, 24 replicated, including 6 newly identified loci. Conditional analyses within loci showed that four loci, including GBA, GAK-DGKQ, SNCA and the HLA region, contain a secondary independent risk variant. In total, we identified and replicated 28 independent risk variants for Parkinson's disease across 24 loci. Although the effect of each individual locus was small, risk profile analysis showed substantial cumulative risk in a comparison of the highest and lowest quintiles of genetic risk (odds ratio (OR) = 3.31, 95% confidence interval (CI) = 2.55-4.30; P = 2 x 10(-16)). We also show six risk loci associated with proximal gene expression or DNA methylation