Oligonucleotide primer sequences used in this study.

1<p>Underlined sequences represent the M13-tail.</p>2<p>Sequences with accession number CR931632 to CR931722 were used for primer design.</p

CT assignment per serotype included in the snapshot.

1<p>Frequency of the CT.</p>2<p>Frequency of the serotype.</p>3<p>Number of mutations with the major CT of the particular serotype.</p>4<p>Silent mutation.</p>5<p>Non-silent mutation.</p>6<p>Number of mutations with the <i>wzh</i> gene segment published by Bentley et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020390#pone.0020390-Bentley1" target="_blank">[21]</a>.</p>7<p>Also perfect match with <i>wzh</i> sequences of 6B isolates AY359459, AY359455, AY359449.</p

Minimum spanning trees of the results obtained by MLVA for serogroup 6.

<p>The minimum spanning tree is based on the entire MLVA database and the results of the pre-vaccination era collection (n = 1154) were plotted within in white nodes. The colored nodes designate the serogoup 6 isolates of this collection. The colors of the nodes indicate the Capsular Type and the serotype is indicated within the figure. For symbols and lines see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020390#pone-0020390-g002" target="_blank">Figure 2</a>.</p

Characteristics of the patients that were infected with the isolates included in the snapshot.

<p>Characteristics of the patients that were infected with the isolates included in the snapshot.</p

Minimum spanning trees of the results obtained by MLVA of 4 major serotypes.

<p>The minimum spanning tree is based on the entire MLVA database and the results of the pre-vaccination era collection (n = 1154) were plotted within in white nodes. The colored nodes designate the isolates of the 4 major serotypes described in the text. The colors of the nodes indicate the Capsular Type. For symbols and lines see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020390#pone-0020390-g002" target="_blank">Figure 2</a>.</p

The frequency of the various alleles present in the 8 BOX loci used for MLVA.

<p>The graphs display the frequency of each allele (number of repeats) for each BOX locus. The Simpson's Diversity Index is indicated for each BOX locus. The number 99 was assigned if no PCR product could be amplified.</p

Serotype distribution of pneumococci causing invasive diesease in patients in the age categories <5 and ≥5 year.

<p>Serotype distribution of pneumococci causing invasive diesease in patients in the age categories <5 and ≥5 year.</p

Minimum spanning tree of the results obtained by MLVA of 1154 pneumococcal isolates.

<p>The minimum spanning tree is based on the entire MLVA database (last accessed on October 20, 2010) with only the results from the current collection plotted within. The circles indicate the types and the size of the circles indicate the number of isolates. Lines linking two types denote a single locus difference between those types. The colors of the circles indicate the serotype of the isolates and complexes were indicated by grey halos.</p