Expression signatures of antisense PIN RNAs and corresponding PIN RNA-overlapped exon pairs relative to their 3' protein-coding exons

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> A subset of 64 pairs of antisense PIN RNAs and corresponding PIN RNA-overlapped exons were identified among the tissue signatures shown in Figure 10 as having correlated patterns of expression: 49 pairs were identified in which the 3' exon of the protein-coding transcript (right panel) follows a similar expression pattern to that of the PIN RNA/PIN RNA-overlapped exon pair (left and central panels); 9 pairs were identified in which the 3' exon of the protein-coding transcript (right panel) does not follow the pattern of tissue expression of the PIN RNA and the corresponding PIN RNA-overlapped exon (left and central panels); 6 pairs in which the PIN RNA (left panel) has an expression pattern inverted in relation to that of the PIN RNA-overlapped exon (central panel). Each line represents a genomic locus covered by three different types of probes (antisense PIN RNA, PIN RNA-overlapped protein-coding exon and 3' protein-coding exon). For each line, expression intensities among the three tissues were normalized within each type of probe and colored as a function of the number of standard deviations from the mean value

Most highly expressed TIN transcripts map to genes related to regulation of transcription

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> TIN RNA expression data from three different human tissues (prostate, liver and kidney) were used to select the protein-coding genes to which the top 40% most highly expressed TIN transcripts map. The BiNGO program was used to identify significantly (≤ 0.05) enriched GO terms within the set of selected protein-coding genes. GO-enriched categories for prostate are shown in color, which is related to the value as indicated by the color-code bar. The exact values for all significantly enriched GO categories are shown in Additional data file 4. GO category 'Regulation of transcription, DNA-dependent' (GO:006355) is the most significantly enriched (= 0.002). Similar results were obtained for liver and kidney (see Additional data file 4). Venn diagram for the 123 unique protein-coding genes belonging to GO:006355 category 'Regulation of transcription, DNA-dependent'. The number of genes in each tissue for which intronic transcription was detected is shown in parenthesis; the numbers of coincident and dissimilar genes among kidney, prostate and liver are shown in the circles

Frequency of exon skipping and abundance of wholly intronic noncoding transcription in RefSeq genes

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Distribution of exon skipping events along spliced RefSeq genes with 7, 8, 9 or 10 exons. Filled squares indicate the average frequency of skipping per exon for genes with evidence of TIN RNAs mapping to their introns. Open squares indicate the average frequency of skipping per exon for genes with no evidence in GenBank that TIN RNAs map to their introns. A significantly higher (< 0.002) frequency of exon skipping was observed for RefSeq genes with TIN RNA transcription. Distribution of TIN transcripts among the introns of RefSeq sequences with 7, 8, 9 or 10 introns selected from GenBank as being outside the 95% confidence level of significance (not correlated) in a Pearson correlation analysis between the abundance of TIN contigs per intron and the intron size (in nt). Bars indicate the average intron size (nt) for this selected set of genes. Triangles indicate the number of TIN contigs per intron for RefSeq genes for the same set

Expression signature of intronic and protein-coding transcripts in human liver, prostate and kidney

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Transcripts with significantly different levels among prostate, kidney and liver samples were selected by a SAM multi-class test (FD

Number of protein-coding, TIN and PIN transcripts expressed in three human tissues

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Different types of transcripts are shown in each panel, and are color-coded as in Figure 3: protein-coding exonic (red bars), antisense TIN (black bars), antisense PIN (blue bars) or sense TIN transcripts (hashed black bars). The total number of probes present in the microarray for each type of transcript is shown with bars marked as 'M'. The number of transcripts expressed in at least one of the three tissues tested is shown with bars marked as 'One'. Transcripts exclusively expressed in each of the three tissues are shown with bars marked as 'L' for liver; 'P' for prostate; or 'K' for kidney. The percentage of expressed transcripts relative to the total number of transcripts probed in the array is indicated at the top of each bar

Expression signatures of wholly intronic RNAs relative to their 3' protein-coding exons

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Cross-referencing of the tissue signatures shown in Figure 10 identified subsets of TIN RNAs that have correlated patterns of expression relative to the 3' protein-coding exon signature from the corresponding genomic loci: 38 pairs were identified in which the 3' exon of the protein-coding transcript (right panel) follows a similar expression pattern to that of the antisense TIN RNA (left panel); 16 pairs were identified in which the 3' exon of the protein-coding transcript (right panel) follows a pattern of tissue expression inverted in relation to that of the antisense TIN RNA (left panel); 64 pairs were identified in which the 3' exon of the protein-coding transcript (right panel) follows a similar expression pattern as that of the sense TIN RNA (left panel); 22 pairs were identified where the 3' exon of the protein-coding transcript (right panel) follows a pattern of tissue expression inverted in relation to that of the sense TIN RNA (left panel). For each line in each panel, expression intensities among the three tissues were normalized within each type of probe and colored as a function of the number of standard deviations from the mean value

Length distribution of exons from RefSeq genes and of partially (PIN) and totally (TIN) intronic noncoding transcripts

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> The curves show the length distribution of three different classes of transcripts reconstructed from genomic mapping and assembly of RefSeq and ESTs from GenBank. Exons of protein-coding RefSeq (red line), TIN (black line) and PIN (blue line) contig sequences. TIN and PIN contigs resulted from assembly of all GenBank unspliced ESTs (in gold) that cluster to a given intronic region in a genomic locus, as shown in the scheme above the curves

Genes with increased intronic transcription in the presence of the RNAP II inhibitor α-amanitin are enriched in the 'Regulation of transcription' GO category

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Gene ontology analysis was performed on protein-coding genes that were shown in the experiment illustrated in Figure 8 to have up-regulated expression of antisense PIN transcripts and sense and antisense TIN transcripts upon exposure to α-amanitin. Significantly (≤ 0.05) enriched GO terms are shown in color, which is related to the value as indicated by the color-code bar. The exact values for all significantly enriched GO categories are shown in Additional data file 7

Effect of RNAP II inhibitor α-amanitin on the abundance of protein-coding, antisense TIN, sense TIN and antisense PIN RNAs

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Lines on each panel represent various transcripts for which the expression levels differed significantly between α-amanitin-treated prostate cells and untreated control cells. Each sample replica is shown in one column. Transcripts were selected by a SAM two-class test (FD

Design and overall performance of the 44 k gene-oriented intron-exon expression oligoarray

<p><b>Copyright information:</b></p><p>Taken from "Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription"</p><p>http://genomebiology.com/2007/8/3/R43</p><p>Genome Biology 2007;8(3):R43-R43.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868932.</p><p></p> Schematic view of the 44 k combined intron-exon expression oligoarray 60-mer probe design. Probe 1 is for the antisense PIN transcripts (blue arrow). Probes 3 and 4 are a pair of reverse complementary sequences designed to detect antisense or sense TIN transcripts (black and hashed black arrows, respectively) in a given locus. Sense exonic probes 2 and 5 are for the protein-coding transcripts (red block and red arrow). Note that the latter were not systematically designed for an exon near the TIN message; in most instances a distant, 3' exon of the gene has been probed instead. Average signal intensity distribution for antisense TIN (solid black line), sense TIN (dashed line), antisense PIN (blue line), or sense protein-coding exonic (red line) probes. Average intensities from six different hybridization experiments with three different human tissues, namely liver, prostate and kidney, are shown. Only probes with intensities above the average negative controls plus 2 SD were considered. The average intensity distribution for probes below this low-limit detection cutoff is shown in the curve marked as 'Not expressed RNAs' (gray line)