EVALUATING THE THERAPEUTIC POTENTIAL OF ORPHAN G-PROTEIN COUPLED RECEPTORS: GPR19 AND GPR17

Ph.DDOCTOR OF PHILOSOPH

Synthesis and spectral studies of some rare earth metal complexes of a heterocyclic mesogenic Schiff-base

<p>A heterocyclic liquid crystalline Schiff's base, <i>N</i>-(4′-(4″-hexyl-1<i>H</i>-1″,2″,3″-triazol))-4-dodecyloxysalicylaldimine (HL), and a series of lanthanide(III) complexes of the type [Ln(LH)₃(NO₃)₃] (Ln^III = La, Pr, Nd, Sm, Eu, Tb, and Dy) have been synthesized and characterized by elemental analyses, mass spectrometry, FTIR, NMR, and UV-Vis spectral techniques. All the complexes involve binding of HL (through phenolate oxygen in zwitter-ionic form) and bidentate chelation of nitrato groups. The ligand exhibits smectic-A (SmA) mesophase over a wide temperature range as evidenced by DSC and polarizing optical microscopic studies. Fluorescence studies show emissions of HL and Tb^III complex.</p

Biological Effects of Naturally Occurring Sphingolipids, Uncommon Variants, and Their Analogs

10.1007/s12017-016-8424-8NEUROMOLECULAR MEDICINE183396-414United State

Coordination of a mesogenic Schiff-base with Mn-II, Co-II, Ni-II, Cu-II and Zn-II: Synthesis, spectral studies and crystal structures

A novel mesogenic (nematic) Schiff-base, N,N′-di-4-(4′-pentyloxybenzoate)salicylidene diaminoethane, H2dpbsde (abbreviated as H2L5) was synthesized and its structure studied. The Schiff-base crystallizes in the non-centrosymmetric space group Pna21 with Z = 4, and the mesogenic isomorphous nickel and copper complexes, [NiL5]2 and [CuL5], in the centrosymmetric space group P21/c with Z = 4. The (L5)2− species coordinates to the metal ions through two phenolate oxygens and two azomethine nitrogens. Both the [NiL5]2 and [CuL5] complexes involve cis-MN2O2 planes; the former complex has a low-spin distorted square-pyramidal geometry with a Ni–Ni bonding of 3.337 Å and the latter, a square-planar geometry

Short-chain fatty acid receptors inhibit invasive phenotypes in breast cancer cells

This article evaluates the effects of enforced overexpression of two known G protein-coupled receptors in two phenotypically distinct breast cancer cell lines

Reduced expression of FFAR2 and FFAR3 in invasive and triple negative breast cancer tissues.

<p>The Curtis breast dataset available sourced from Oncomine.org was analyzed for the expression of (A) <i>FFAR2</i> and (B) <i>FFAR3</i> mRNA in 1992 breast carcinoma and 144 normal breast samples. Whiskers represent the minimum to maximum values. One-way ANNOVA was performed to calculate significance. <i>FFAR2</i> expression is reduced in some classes, while <i>FFAR3</i> was reduced in all invasive tumor types. The dataset was analyzed for (C) <i>FFAR2</i> and (D) <i>FFAR3</i> expression level in 144 normal breast tissue samples and in 250 triple negative breast tumors. Expression of both <i>FFAR2</i> and <i>FFAR3</i> were significantly reduced in triple negative breast tumors. (E) Expression data obtained from the Cancer Cell Line Encyclopedia show that among 57 breast cancer cell lines, there is a significant positive correlation between <i>FFAR2</i> and <i>CDH1</i> mRNA levels (Pearson r = 0.516, P <0.001). (F) Expression data obtained from the Human Protein Atlas show that among 1075 primary breast tumor samples, there is a significant positive correlation between <i>FFAR2</i> and <i>CDH1</i> mRNA levels (Pearson r = 0.138, P <0.001).</p

Anti-invasive phenotype in FFAR2- and FFAR3-overexpressing MDA-MD-231 cells.

<p>(A) MDA-MB-231 cells were seeded in to the upper chambers of Biocoat Matrigel transwells, then treated with vehicle control or with 1 mM propionate for 24 hours. Representative images show cells that have invaded through the matrix. (B) Quantitation of the invasion assay in (A) shows a significant ligand-mediated reduction in the invasiveness in cells overexpressing FFAR2 and FFAR3, but not vector control cells (N = 3). (C) MDA-MB-231 cells were labeled with rhodamine-phalloidin in order to visualize actin filaments. Representative images are shown. (D) Quantification of cells from (C) that demonstrate the presence of stress fibers (N = 3). (*p< 0.05, **p< 0.01).</p