Early specification of sensory neuron fate revealed by expression and function of neurogenins in the chick embryo

The generation of sensory and autonomic neurons from the neural crest requires the functions of two classes of basic helix-loop-helix (bHLH) transcription factors, the Neurogenins (NGNs) and MASH-1, respectively (Fode, C., Gradwohl, G., Morin, X., Dierich, A., LeMeur, M., Goridis, C. and Guillemot, F. (1998) Neuron 20, 483-494; Guillemot, F., Lo, L.-C., Johnson, J. E., Auerbach, A., Anderson, D. J. and Joyner, A. L. (1993) Cell 75, 463-476; Ma, Q., Chen, Z. F., Barrantes, I. B., de la Pompa, J. L. and Anderson, D. J. (1998 Neuron 20, 469-482). We have cloned two chick NGNs and found that they are expressed in a subset of neural crest cells early in their migration. Ectopic expression of the NGNs in vivo biases migrating neural crest cells to localize in the sensory ganglia, and induces the expression of sensory neuron-appropriate markers in non-sensory crest derivatives. Surprisingly, the NGNs can also induce the expression of multiple pan-neuronal and sensory-specific markers in the dermomyotome, a mesodermal derivative. Taken together, these data suggest that a subset of neural crest cells may already be specified for a sensory neuron fate early in migration, as a consequence of NGN expression

Identification of dividing, determined sensory neuron precursors in the mammalian neural crest

Sensory and autonomic neurons of the vertebrate peripheral nervous system are derived from the neural crest. Here we use the expression of lineage-specific transcription factors as a means to identify neuronal subtypes that develop in rat neural crest cultures grown in a defined medium. Sensory neurons, identified by expression of the POU-domain transcription factor Brn-3.0, develop from dividing precursors that differentiate within 2 days following emigration from the neural tube. Most of these precursors generate sensory neurons even when challenged with BMP2, a factor that induces autonomic neurogenesis in many other cells in the explants. Moreover, BMP2 fails to prevent expression of the sensory-specific basic helix-loop-helix (bHLH) transcription factors neurogenin1, neurogenin2 and neuroD, although it induces expression of the autonomic-specific bHLH factor MASH1 and the paired homeodomain factor Phox2a in other cells. These data suggest that there are mitotically active precursors in the mammalian neural crest that can generate sensory neurons even in the presence of a strong autonomic-inducing cue. Further characterization of the neurons generated from such precursors indicates that, under these culture conditions, they exhibit a proprioceptive and/or mechanosensory, but not nociceptive, phenotype. Such precursors may therefore correspond to a lineally (Frank, E. and Sanes, J. (1991) Development 111, 895-908) and genetically (Ma, Q., Fode, C., Guillemot, F. and Anderson, D. J. (1999) Genes Dev. 13, in press) distinct subset of early-differentiating precursors of large-diameter sensory neurons identified in vivo

Induction and repression of mammalian achaete-scute homologue (MASH) gene expression during neuronal differentiation of P19 embryonal carcinoma cells

MASH1 and MASH2, mammalian homologues of the Drosophila neural determination genes achaete-scute, are members of the basic helix-loop-helix (bHLH) family of transcription factors. We show here that murine P19 embryonal carcinoma cells can be used as a model system to study the regulation and function of these genes. MASH1 and MASH2 display complementary patterns of expression during the retinoic-acid-induced neuronal differentiation of P19 cells. MASH1 mRNA is undetectable in undifferentiated P19 cells but is induced to high levels by retinoic acid coincident with neuronal differentiation. In contrast, MASH2 mRNA is expressed in undifferentiated P19 cells and is repressed by retinoic acid treatment. These complementary expression patterns suggest distinct functions for MASH1 and MASH2 in development, despite their sequence homology. In retinoic-acid-treated P19 cells, MASH1 protein expression precedes and then overlaps expression of neuronal markers. However, MASH1 is expressed by a smaller proportion of cells than expresses such markers. MASH1 immunoreactivity is not detected in differentiated cells displaying a neuronal morphology, suggesting that its expression is transient. These features of MASH1 expression are similar to those observed in vivo, and suggest that P19 cells represent a good model system in which to study the regulation of this gene. Forced expression of MASH1 was achieved in undifferentiated P19 cells by transfection of a cDNA expression construct. The transfected cells expressing exogenous MASH1 protein contained E-box-binding activity that could be super-shifted by an anti-MASH1 antibody, but exhibited no detectable phenotypic changes. Thus, unlike myogenic bHLH genes, such as MyoD, which are sufficient to induce muscle differentiation, expression of MASH1 appears insufficient to promote neurogenesis

Evaluation of Swale Design

Swales are designed to infiltrate runoff from intermittent storm events. Present design methodologies have resulted in swales which operate under several conditions; these conditions are soil, vegetation, climatic and geographical location dependent. To attain a swale design which considers and accounts for the important factors under Florida conditions, adequate assessment of rainfall, overland, flow, infiltration and soil moisture must be given priority treatment. Several roadside and residential swales were studied and relationships were drawn for soil moisture, porosity and infiltration rates. A design methodology is included and computer modeling infiltration aids in the design

Electrophysiological biomarkers of chemotherapy-related cognitive impairment in hematological malignancy patients

Multiple cancer populations frequently report cognitive impairment following treatment with chemotherapy agents (“chemo-brain”). Impaired neuropsychological performance is commonly reported in cognitive domains of attention and executive function. Understanding neural mechanisms underlying cognitive impairments is essential to developing prevention and rehabilitation strategies. Brain imaging studies frequently show chemotherapy-related impairments within the attentional control network, which is comprised of a constellation of cortical regions that govern reportedly impaired cognitive functions. In the current dissertation research, I developed a novel electrophysiology battery aimed at recording near-instantaneous neural activity within the attentional control network during cognitive task performance. Cancer patients diagnosed with hematological malignancy (e.g. lymphoma, myeloma) completed three longitudinal assessments: (1) prior to starting chemotherapy, and following (2) one-month and (3) three-months of chemotherapy. Comparison groups included patients not receiving chemotherapy and demographically-matched healthy controls. Outcome measures provide initial support for contributions from both tumor biology and chemotherapy toxicity to functional changes in attentional control network activity. Furthermore, both cancer groups showed evidence for reduced information processing capacity while completing a simulation of naturalistic driving behavior. These results provide a unique platform for understanding basic neural mechanisms and translational impacts of attentional control impairment in cancer patients. Future large-scale studies must be committed to confirming these results, and further innovative work is necessary to confirm the link between previous brain imaging studies and cognitive electrophysiology measures used here. Innovative chemotherapies are improving survivorship among cancer patients. Thus, ensuring long-term quality of life among our growing cancer survivor population is paramount to achieving the highest level of public health and safety

Survey of Biosecurity Practices Utilized by Veterinarians Working with Farm Animal Species

Biosecurity programs can be discussed from many perspectives. In many cases, biosecurity may be regulated state or federal programs with law enforcement - the goals of which are to protect the national livestock resources and protect consumer interests. Alternatively, these programs may be producer level programs that are voluntarily established for protection of personal livestock investments. A core principle in the definition of biosecurity is the desire to prevent the introduction of disease. When infectious disease is present on a farm or in a region, the term biocontainment may be used to describe programs aimed at confinement of the diseases to that farm or region such that it does not spread to other farms. Veterinarians are intimately involved in the maintenance of health and in the diagnosis and treatment of disease. When confronting an ongoing disease diagnosis and treatment situation, biocontainment can be readily implemented because the effects are apparent. However, when working to maintain a healthy herd, biosecurity practices may be more challenging to enforce because the threat is neither apparent nor seems eminent. An additional perspective on biosecurity relates to zoonotic diseases. Animal handlers and veterinarians are at risk for disease transmission from the animal to humans as part of their normal daily work habits. This chronic and persistent exposure may lead to complacency regarding risk if the people involved have never knowingly suffered disease. Thus, biosecurity can be a challenging concept for farm managers and veterinarians to apply in field settings

High-resolution antenna near-field imaging and sub-THz measurements with a small atomic vapor-cell sensing element

Atomic sensing and measurement of millimeter-wave (mmW) and THz electric fields using quantum-optical EIT spectroscopy of Rydberg states in atomic vapors has garnered significant interest in recent years towards the development of atomic electric-field standards and sensor technologies. Here we describe recent work employing small atomic vapor cell sensing elements for near-field imaging of the radiation pattern of a K$_u$-band horn antenna at 13.49 GHz. We image fields at a spatial resolution of $\lambda/10$ and measure over a 72 to 240 V/m field range using off-resonance AC-Stark shifts of a Rydberg resonance. The same atomic sensing element is used to measure sub-THz electric fields at 255 GHz, an increase in mmW-frequency by more than one order of magnitude. The sub-THz field is measured over a continuous $\pm$100 MHz frequency band using a near-resonant mmW atomic transition