Biopiracy and states’ sovereignty over their biological resources

In the last several decades, indigenous communities had to contend with the misappropriation of their biological resources and associated traditional knowledge (TK) through the inappropriate exercise of intellectual property rights (IPRs). The advent of modern biotechnology has intensified this problem leaving indigenous communities increasingly vulnerable. As a counter measure, the Convention on Biological Diversity (CBD) was adopted in 1992, proclaiming the sovereignty of states over their biological resources, and requiring their consent and the equitable sharing of benefits on mutually agreed terms as conditions for access. On October 29, 2010, the Nagoya Protocol was adopted to implement these provisions. Nevertheless, the CBD has attracted critical comments from those opposed to the idea of state sovereignty over biological resources, especially when the exercise of sovereignty transcends a state’s territorial borders. Two alternative doctrines; “the common heritage of mankind”, and “the global commons”, have been canvassed. This paper set out to analyse these arguments together with the alternative doctrines, in order to determine whether state sovereignty over biological resources as proclaimed by the CBD is justified. The merits of each doctrine were examined against the background of the problems presented to indigenous communities by the trinity of biopiracy, IPRs and modern biotechnology. The paper found that the doctrine of state sovereignty over biological resources, whilst having its limitations, is not only normatively justified, but is also, comparatively more capable of helping to protect the biological resources and associated TK of indigenous communities against piracy.Key words: Indigenous communities, biological resources, traditional knowledge, biopiracy, Convention on Biological Diversity (CBD), plant breeders’ rights, patents, sovereignty

Public attitude towards modern biotechnology

This article reviews the literature related to the main idea of the study, rooting from the definition of biotechnology, global status of commercialized biotechnology products, and global and local public attitudes towards modern biotechnology and past models for attitude towards modern biotechnology. The first section of the review will be the in-depth-discussion regarding the definition of modern biotechnology according to several established international organizations, followed by global status of commercialized biotechnology products which will emphasize on how modern biotechnology is classified and which area are being focused more by the stakeholders, and global and local public attitudes towards modern biotechnology based on previous studies. Last but not least, the final section is credited to past studies related to attitudes and past models of public attitudes towards biotechnology, both globally and locally. A developing country like Malaysia was chosen in this article as an example of the case study related to local situation of modern biotechnology.Key words: Modern biotechnology, genetically modified (GM), public attitude, Malaysi

Anti-amoebic activity of acyclic and cyclic-samarium complexes on Acanthamoeba.

This work investigated the anti-amoebic activity of two samarium (Sm) complexes, the acyclic complex [bis(picrato)(pentaethylene glycol)samarium(III)] picrate-referred to as [Sm(Pic)2(EO5)](Pic)-and the cyclic complex [bis(picrato)(18-crown-6)samarium(III)] picrate-referred to as [Sm(Pic)2(18C6)](Pic). Both Sm complexes caused morphological transformation of the protozoa Acanthamoeba from its native trophozoite form carrying a spine-like structure called acanthopodia, to round-shaped cells with loss of the acanthopodia structure, a trademark response to environmental stress. Further investigation, however, revealed that the two forms of the Sm complexes exerted unique cytotoxicity characteristics. Firstly, the IC50of the acyclic complex (0.7 μg/mL) was ~ 10-fold lower than IC50of the cyclic Sm complex (6.5 μg/mL). Secondly, treatment of the Acanthamoeba with the acyclic complex caused apoptosis of the treated cells, while the treatment with the cyclic complex caused necrosis evident by the leakage of the cell membrane. Both treatments induced DNA damage in Acanthamoeba. Finally, a molecular docking simulation revealed the potential capability of the acyclic complex to form hydrogen bonds with profilin-a membrane protein present in eukaryotes, including Acanthamoeba, that plays important roles in the formation and degradation of actin cytoskeleton. Not found for the cyclic complex, such potential interactions could be the underlying reason, at least in part, for the much higher cytotoxicity of the acyclic complex and also possibly, for the observed differences in the cytotoxicity traits. Nonetheless, with IC50values of < 10 μg/mL, both the acyclic and cyclic Sm complexes feature a promising potential as cytotoxic agents to fight amoebic infections

Effect of demographic variables on public attitudes towards genetically modified insulin

Earlier studies on public attitude and risk perception have concluded that the public’s attitudes towards biotechnology was primarily driven by several factors such as familiarity, perceived benefits, perceived risks, risk acceptance, moral concerns and encouragement. Demographic characteristics have been known to affect attitudes towards science. The purpose of this paper is to compare the attitude of the Malaysian public towards genetically modified (GM) insulin across several background variables such as religion, race, education level and age. A survey was carried out on 1017 respondents stratified according to various stakeholder groups in the Klang Valley region. Analyses of Variance (ANOVAs) showed significant differences in the mean scores for familiarity of GM insulin across religions, races and ages but not across education levels and gender. Both perceived benefits and perceived risks were found to differ across races, education levels and gender but not across religions and ages. On the other hand, moral concern was found to differ in all four background variables except gender while risk acceptance differed across races and gender and encouragement only differed across education  levels. In conclusion, background variables do have a significant effect on some of the dimensions of Malaysians’ attitudes towards modern biotechnology. The research findings will be useful for understanding the effect of background variables on public attitudes towards the application of gene technology in medicine. More in-depth empirical studies should be carried out to understand the underlying causes behind the differences.Key words: Attitude, gene technology, medicine, GM (genetically modified) insulin, background variables, Malaysia

The need for biosafety regulation in developing countries: Benefits and controversies

Nowadays, the rapid development of biotechnology has become a main concern for a larger part of the world. It has become one of the most promising fields which guarantee returns to businesses and offers benefits to the society. When dealing with biotechnology, the first issue that comes to mind is the safeness of the technology from tip to toe, that is, the safeness of the products of biotechnology, how they can be used on human beings and animal, and their effects on the environment. The objective of this paper is to assess the needs and adequacy of the regulation in developing countries compared to the developed countries. In order to address these concerns, governments have adopted appropriate regulations to ensure the safety of the biotechnology products, and to protect not just human but the environment universally. This paper will discuss those regulations, especially as adopted by developing countries along with their implications. It is hoped that the paper will recover the lack of the regulations in relation to developed country.Key words: Biotechnology, biosafety, developing countries, benefits, risks and controversies

Characterisation and induction of tissue-resident gamma delta T-cells to target hepatocellular carcinoma

Immunotherapy is now the standard of care for advanced hepatocellular carcinoma (HCC), yet many patients fail to respond. A major unmet goal is the boosting of T-cells with both strong HCC reactivity and the protective advantages of tissue-resident memory T-cells (TRM). Here, we show that higher intratumoural frequencies of γδ T-cells, which have potential for HLA-unrestricted tumour reactivity, associate with enhanced HCC patient survival. We demonstrate that γδ T-cells exhibit bona fide tissue-residency in human liver and HCC, with γδTRM showing no egress from hepatic vasculature, persistence for >10 years and superior anti-tumour cytokine production. The Vγ9Vδ2 T-cell subset is selectively depleted in HCC but can efficiently target HCC cell lines sensitised to accumulate isopentenyl-pyrophosphate by the aminobisphosphonate Zoledronic acid. Aminobisphosphonate-based expansion of peripheral Vγ9Vδ2 T-cells recapitulates a TRM phenotype and boosts cytotoxic potential. Thus, our data suggest more universally effective HCC immunotherapy may be achieved by combining aminobisphosphonates to induce Vγ9Vδ2TRM capable of replenishing the depleted pool, with additional intratumoural delivery to sensitise HCC to Vγ9Vδ2TRM-based targeting

A day in the life of marine sulfonates

Lab-based studies, combined with metatranscriptomic and metabolomic field analyses, reveal important diel-linked roles for sulfonates in the major classes of phytoplankton that produce them, and in the environment in which they feed ubiquitous heterotrophic bacteri

Targeting human Acyl-CoA:cholesterol acyltransferase as a dual viral and T cell metabolic checkpoint

Determining divergent metabolic requirements of T cells, and the viruses and tumours they fail to combat, could provide new therapeutic checkpoints. Inhibition of acyl-CoA:cholesterol acyltransferase (ACAT) has direct anti-carcinogenic activity. Here, we show that ACAT inhibition has antiviral activity against hepatitis B (HBV), as well as boosting protective anti-HBV and anti-hepatocellular carcinoma (HCC) T cells. ACAT inhibition reduces CD8+ T cell neutral lipid droplets and promotes lipid microdomains, enhancing TCR signalling and TCR-independent bioenergetics. Dysfunctional HBV- and HCC-specific T cells are rescued by ACAT inhibitors directly ex vivo from human liver and tumour tissue respectively, including tissue-resident responses. ACAT inhibition enhances in vitro responsiveness of HBV-specific CD8+ T cells to PD-1 blockade and increases the functional avidity of TCR-gene-modified T cells. Finally, ACAT regulates HBV particle genesis in vitro, with inhibitors reducing both virions and subviral particles. Thus, ACAT inhibition provides a paradigm of a metabolic checkpoint able to constrain tumours and viruses but rescue exhausted T cells, rendering it an attractive therapeutic target for the functional cure of HBV and HBV-related HCC

Epidemiology of Coxiella burnetii infection in Africa: a OneHealth systematic review

Background: Q fever is a common cause of febrile illness and community-acquired pneumonia in resource-limited settings. Coxiella burnetii, the causative pathogen, is transmitted among varied host species, but the epidemiology of the organism in Africa is poorly understood. We conducted a systematic review of C. burnetii epidemiology in Africa from a “One Health” perspective to synthesize the published data and identify knowledge gaps.&lt;p&gt;&lt;/p&gt; Methods/Principal Findings: We searched nine databases to identify articles relevant to four key aspects of C. burnetii epidemiology in human and animal populations in Africa: infection prevalence; disease incidence; transmission risk factors; and infection control efforts. We identified 929 unique articles, 100 of which remained after full-text review. Of these, 41 articles describing 51 studies qualified for data extraction. Animal seroprevalence studies revealed infection by C. burnetii (&#8804;13%) among cattle except for studies in Western and Middle Africa (18–55%). Small ruminant seroprevalence ranged from 11–33%. Human seroprevalence was &#60;8% with the exception of studies among children and in Egypt (10–32%). Close contact with camels and rural residence were associated with increased seropositivity among humans. C. burnetii infection has been associated with livestock abortion. In human cohort studies, Q fever accounted for 2–9% of febrile illness hospitalizations and 1–3% of infective endocarditis cases. We found no studies of disease incidence estimates or disease control efforts.&lt;p&gt;&lt;/p&gt; Conclusions/Significance: C. burnetii infection is detected in humans and in a wide range of animal species across Africa, but seroprevalence varies widely by species and location. Risk factors underlying this variability are poorly understood as is the role of C. burnetii in livestock abortion. Q fever consistently accounts for a notable proportion of undifferentiated human febrile illness and infective endocarditis in cohort studies, but incidence estimates are lacking. C. burnetii presents a real yet underappreciated threat to human and animal health throughout Africa.&lt;p&gt;&lt;/p&gt