A new method for the detection of singular points in fingerprint images

Automatic biometric identification based on fingerprintsis still one of the most reliable identification method in criminaland forensic applications. A critical step in fingerprintanalysis without human intervention is to automatically andreliably extract singular points from the input fingerprintimages. These singular points (cores and deltas) not onlyrepresent the characteristics of local ridge patterns but alsodetermine the topological structure (i.e., fingerprint type)and largely influence the orientation field. Poincaré Indexbasedmethods are one of the most common for singularpoints detection. However, these methods usually result inmany spurious detections. Therefore, we propose an enhancedversion of the method presented by Zhou et al. [13]that introduced a feature called DORIC to improve the detection.Our principal contribution lies in the adoption of asmoothed orientation field and in the formulation of a newalgorithm to analyze the DORIC feature. Experimental resultsshow that the proposed algorithm is accurate and robust,giving better results than the best reported results sofar, with improvements in the range of 5% to 7%

Evaluation of Internal Markers for Estimating Duodenal Digesta Flow in Ruminants: Acid Detergent Fibre and Lignin Disappearance at the Lower Gastrointestinal Tract

Most of published studies carried out for estimating organic matter (OM) rumen digestibility (OMRD) use research an-imals fitted with simple t-type cannulas and an external or internal marker for estimating the duodenal digesta flow. Compared to external, the internal markers have the advan-tage of occurring naturally in diet and, consequently, they flow intimately associated with digesta (Titgemeyer 1997). Porter and Singleton (1971a) reported from a study with sheep fitted with re-entrant duodenal cannula that lignin degradation takes place entirely in the stomach. Thus, in digestibility studies where total faeces output is measured, duodenal digesta flow may be estimated based on both faeces output and the ratio of lignin concentration in faeces and in duodenal digesta. However, sulphuric acid lignin (ADL) is present in low concentrations in duodenal digesta and the precision of duodenal flow estimates is usually compromised. This study evaluated the disappearance at the lower gastrointestinal tract and, consequently, the po-tential use of acid detergent fibre (ADF), in comparison with ADL, as an internal marker for estimating duodenal digesta flow in cattle

Polyphenol extraction by deep eutectic solvent for valorisation of portuguese green tea and their impact on chitosan-based films properties

The current demand for antioxidant active packaging, that is achieved by introducing antioxidants into food packaging material, is increasing due to both the unquestionable advantages compared to the addition of antioxidants directly to the food and the tendency to consume healthy and fresh products. When developing active packaging materials, research has been focused on natural and biodegradable polymers (such as polysaccharides like chitosan) containing polyphenol compounds (as bio preservative) in their formulation. Due to their rare solvation properties, the natural deep eutectic solvents (NADES), are considered good solvents to be used as an extraction medium of bioactive products. Moreover, the search for the use of ecological solvents for this field has intensified due to their acceptable toxicity profile and chemical diversity. The use of these inexpensive, non-volatile, and nonaqueous biodegradable solvents, complying with the Green Chemistry principles, could potentially improve the stability of the polyphenolic compounds allowing to retard food spoilage for packaging material, Catechins (components of green tea (GT)), a polyphenolic group known for its high value-added antioxidant properties, have been associated with health-promoting effects. In this sense, the incorporation of GT components for food application purposes and as active ingredient in packaging materials can be expected to improve food functionality and availability, while performing a dual role (antioxidant and antimicrobial agent) and possibly also having low-cost advantages. In this work, for the first time, a ternary deep eutectic solvent (ChCl/glycerol/lactic acid) was used as an environmentally friendly media for the extraction of polyphenolic compounds from green tea. The extraction solution was compared with traditional method and used to improve chitosan film properties. The casting method used to produce films with and without GT/NADES plasticizer. Transparent films were obtained and evaluated in terms of mechanical, water resistance, optical and microstructural properties. The results were compared with those obtained for chitosan films containing binary deep eutectic solvents (ChCl/glycerol and ChCl/lactic acid, with and without GT)) as plasticizers in their formulations.The work was supported through the project UIDB/50006/2020, funded by FCT/MCTES through national funds and by the AgriFood XXI I&D&I project (NORTE-01-0145-FEDER-000041 co-financed by European Regional Development Fund (ERDF), through the NORTE 2020. N Teixeira and Hiléia Souza thanks FCT for CEECIND/00025/2018/CP1545/CT0009 and PTDC/OCE-ETA/31250/2017 project respectively.info:eu-repo/semantics/publishedVersio

nutrition and performance in football

Nutrition is an undeniable part of promoting health and performance among football (soccer) players. Nevertheless, nutritional strategies adopted in elite football can vary significantly depending on culture, habit and practical constraints and might not always be supported by scientific evidence. Therefore, a group of 28 Portuguese experts on sports nutrition, sports science and sports medicine sought to discuss current practices in the elite football landscape and review the existing evidence on nutritional strategies to be applied when supporting football players. Starting from understanding football?s physical and physiological demands, five different moments were identified: preparing to play, match-day, recovery after matches, between matches and during injury or rehabilitation periods. When applicable, specificities of nutritional support to young athletes and female players were also addressed. The result is a set of practical recommendations that gathered consensus among involved experts, highlighting carbohydrates periodisation, hydration and conscious use of dietary supplements.D915-7373-ED16 | Cesar LeaoN/

MAMMALS IN PORTUGAL : A data set of terrestrial, volant, and marine mammal occurrences in P ortugal

Mammals are threatened worldwide, with 26% of all species being includedin the IUCN threatened categories. This overall pattern is primarily associatedwith habitat loss or degradation, and human persecution for terrestrial mam-mals, and pollution, open net fishing, climate change, and prey depletion formarine mammals. Mammals play a key role in maintaining ecosystems func-tionality and resilience, and therefore information on their distribution is cru-cial to delineate and support conservation actions. MAMMALS INPORTUGAL is a publicly available data set compiling unpublishedgeoreferenced occurrence records of 92 terrestrial, volant, and marine mam-mals in mainland Portugal and archipelagos of the Azores and Madeira thatincludes 105,026 data entries between 1873 and 2021 (72% of the data occur-ring in 2000 and 2021). The methods used to collect the data were: live obser-vations/captures (43%), sign surveys (35%), camera trapping (16%),bioacoustics surveys (4%) and radiotracking, and inquiries that represent lessthan 1% of the records. The data set includes 13 types of records: (1) burrowsjsoil moundsjtunnel, (2) capture, (3) colony, (4) dead animaljhairjskullsjjaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8),observation in shelters, (9) photo trappingjvideo, (10) predators dietjpelletsjpine cones/nuts, (11) scatjtrackjditch, (12) telemetry and (13) vocalizationjecholocation. The spatial uncertainty of most records ranges between 0 and100 m (76%). Rodentia (n=31,573) has the highest number of records followedby Chiroptera (n=18,857), Carnivora (n=18,594), Lagomorpha (n=17,496),Cetartiodactyla (n=11,568) and Eulipotyphla (n=7008). The data setincludes records of species classified by the IUCN as threatened(e.g.,Oryctolagus cuniculus[n=12,159],Monachus monachus[n=1,512],andLynx pardinus[n=197]). We believe that this data set may stimulate thepublication of other European countries data sets that would certainly contrib-ute to ecology and conservation-related research, and therefore assisting onthe development of more accurate and tailored conservation managementstrategies for each species. There are no copyright restrictions; please cite thisdata paper when the data are used in publications.info:eu-repo/semantics/publishedVersio

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio

Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & Nemésio 2007; Donegan 2008, 2009; Nemésio 2009a–b; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on 18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based researchers who signed it in the short time span from 20 September to 6 October 2016

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Mammals in Portugal: a data set of terrestrial, volant, and marine mammal occurrences in Portugal

Mammals are threatened worldwide, with ~26% of all species being included in the IUCN threatened categories. This overall pattern is primarily associated with habitat loss or degradation, and human persecution for terrestrial mammals, and pollution, open net fishing, climate change, and prey depletion for marine mammals. Mammals play a key role in maintaining ecosystems functionality and resilience, and therefore information on their distribution is crucial to delineate and support conservation actions. MAMMALS IN PORTUGAL is a publicly available data set compiling unpublished georeferenced occurrence records of 92 terrestrial, volant, and marine mammals in mainland Portugal and archipelagos of the Azores and Madeira that includes 105,026 data entries between 1873 and 2021 (72% of the data occurring in 2000 and 2021). The methods used to collect the data were: live observations/captures (43%), sign surveys (35%), camera trapping (16%), bioacoustics surveys (4%) and radiotracking, and inquiries that represent less than 1% of the records. The data set includes 13 types of records: (1) burrows | soil mounds | tunnel, (2) capture, (3) colony, (4) dead animal | hair | skulls | jaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8), observation in shelters, (9) photo trapping | video, (10) predators diet | pellets | pine cones/nuts, (11) scat | track | ditch, (12) telemetry and (13) vocalization | echolocation. The spatial uncertainty of most records ranges between 0 and 100 m (76%). Rodentia (n =31,573) has the highest number of records followed by Chiroptera (n = 18,857), Carnivora (n = 18,594), Lagomorpha (n = 17,496), Cetartiodactyla (n = 11,568) and Eulipotyphla (n = 7008). The data set includes records of species classified by the IUCN as threatened (e.g., Oryctolagus cuniculus [n = 12,159], Monachus monachus [n = 1,512], and Lynx pardinus [n = 197]). We believe that this data set may stimulate the publication of other European countries data sets that would certainly contribute to ecology and conservation-related research, and therefore assisting on the development of more accurate and tailored conservation management strategies for each species. There are no copyright restrictions; please cite this data paper when the data are used in publications