Theoretical study of the chemiluminescence of the Al + H2O reaction

We performed surface hopping simulations of Al + H2O collisions by a direct semiempirical method, reproducing the conditions of previous beam-gas experiments. We observed the formation of the HAlOH species, that dissociates to AlOH + H after a lifetime of about 0.6 ps. This species undergoes nonadiabatic transitions to its first excited state and is responsible for chemiluminescence in the visible range, while the Al−H2O complex emits in the infrared. The computed emission band in the visible is red-shifted with respect to the experimental one, because of slight inaccuracies of the potential energy surfaces. However, collisions with more water molecules and exciplex formation with excited Al(2S, 4P) atoms may also contribute to the short wavelength emission, as we show by accurate ab initio calculations

Cell senescence is an antiviral defense mechanism

Cellular senescence is often considered a protection mechanism triggered by conditions that impose cellular stress. Continuous proliferation, DNA damaging agents or activated oncogenes are well-known activators of cell senescence. Apart from a characteristic stable cell cycle arrest, this response also involves a proinflammatory phenotype known as senescence-associated secretory phenotype (SASP). This, together with the widely known interference with senescence pathways by some oncoviruses, had led to the hypothesis that senescence may also be part of the host cell response to fight virus. Here, we evaluate this hypothesis using vesicular stomatitis virus (VSV) as a model. Our results show that VSV replication is significantly impaired in both primary and tumor senescent cells in comparison with non-senescent cells, and independently of the stimulus used to trigger senescence. Importantly, we also demonstrate a protective effect of senescence against VSV in vivo. Finally, our results identify the SASP as the major contributor to the antiviral defense exerted by cell senescence in vitro, and points to a role activating and recruiting the immune system to clear out the infection. Thus, our study indicates that cell senescence has also a role as a natural antiviral defense mechanism.This work was supported by Grant BFU2014- 58530-P from the Ministry of Economy and Competitiveness of Spain and EU-FEDER. MC is a Miguel Servet investigator supported by an ISCIII and EU-FEDER grant (PI14/00554). AEM is a recipient of a FPI predoctoral fellowship from the Ministry of Economy and Competitiveness of Spain. We are grateful to Dr. I Ventoso for providing the anti-VSV-G antibody.Peer reviewe

A movie of a star: multi-epoch VLBA imaging of the SiO masers towards the Mira variable TX Cam

We describe an observing campaign using the Very Long Baseline Array (VLBA) to monitor the time-evolution of the v=1, J=1-0 SiO maser emission towards the Mira variable TX Cam. The data reported here cover the period 1997 May 24 to 1999 February 19, during which the SiO maser emission was imaged at approximately bi-weekly intervals. The result is an animated movie at an angular resolution of ~500 microarcsec, over a full pulsation period, of the gas motions in the near circumstellar environment of this star, as traced by the SiO maser emission. This paper serves to release the movie and is the first in a series concerning the scientific results from this observing campaign. In this paper, we discuss the global proper motion of the SiO maser emission as a function of pulsation phase. We measure a dominant expansion mode between optical phases $\phi \sim 0.7-1.5$ confirming ballistic deceleration, and compare this to predictions from existing pulsation models for late-type, evolved stars. Local infall and outflow motions are superimposed on the dominant expansion mode, and non-radial local gas motions are also evident for individual SiO maser components. The overall morphology and evolution of the SiO emission deviates significantly from spherical symmetry, with important implications for models of pulsation kinematics in the near-circumstellar environments of Mira variables.Comment: Mpeg movie can be obtained from http://www.jb.man.ac.uk/~pdiamond/txcam_movie.mpe

The repertoire of ICE in prokaryotes underscores the unity, diversity, and ubiquity of conjugation

Horizontal gene transfer shapes the genomes of prokaryotes by allowing rapid acquisition of novel adaptive functions. Conjugation allows the broadest range and the highest gene transfer input per transfer event. While conjugative plasmids have been studied for decades, the number and diversity of integrative conjugative elements (ICE) in prokaryotes remained unknown. We defined a large set of protein profiles of the conjugation machinery to scan over 1,000 genomes of prokaryotes. We found 682 putative conjugative systems among all major phylogenetic clades and showed that ICEs are the most abundant conjugative elements in prokaryotes. Nearly half of the genomes contain a type IV secretion system (T4SS), with larger genomes encoding more conjugative systems. Surprisingly, almost half of the chromosomal T4SS lack co-localized relaxases and, consequently, might be devoted to protein transport instead of conjugation. This class of elements is preponderant among small genomes, is less commonly associated with integrases, and is rarer in plasmids. ICEs and conjugative plasmids in proteobacteria have different preferences for each type of T4SS, but all types exist in both chromosomes and plasmids. Mobilizable elements outnumber self-conjugative elements in both ICEs and plasmids, which suggests an extensive use of T4SS in trans. Our evolutionary analysis indicates that switch of plasmids to and from ICEs were frequent and that extant elements began to differentiate only relatively recently. According to the present results, ICEs are the most abundant conjugative elements in practically all prokaryotic clades and might be far more frequently domesticated into non-conjugative protein transport systems than previously thought. While conjugative plasmids and ICEs have different means of genomic stabilization, their mechanisms of mobility by conjugation show strikingly conserved patterns, arguing for a unitary view of conjugation in shaping the genomes of prokaryotes by horizontal gene transfer

ASSISTÊNCIA À PARTURIENTE SUBMETIDA À INFUSÃO ENDOVENOSA CONTÍNUA DE OCITOCINA

This paper emphasizes the importance of specialized nursing assistance to the woman in labor when contractions are stimulated by infusion containing oxytocin. The author describes the procedures recommended in the litterature for the treatment and for control of mother and child conditions during the firts phase of delivery.No presente trabalho destaca-se a importância da assistência criteriosa à parturiente submetida à estimulação do parto com infusão endovenosa contínua de ocitocina. Para isso, descreve-se os procedimentos recomendados por diversos autores para a administração correta da infusão de ocitocina, assim como para a avaliação das condições materna e fetal, no evolver do primeiro período do parto