Green Synthesized of Thymus vulgaris Chitosan Nanoparticles Induce Relative WRKY-Genes Expression in Solanum lycopersicum against Fusarium solani, the Causal Agent of Root Rot Disease

: Fusarium solani is a plant pathogenic fungus that causes tomato root rot disease and yield losses in tomato production. The current study's main goal is testing the antibacterial efficacy of chitosan nanoparticles loaded with Thyme vulgaris essential oil (ThE-CsNPs) against F. solani in vitro and in vivo. GC-MS analysis was used to determine the chemical constituents of thyme EO. ThE-CsNPs were investigated using transmission electron microscopy before being physicochemically characterized using FT-IR. ThE-CsNPs were tested for antifungal activity against F. solani mycelial growth in vitro. A pot trial was conducted to determine the most effective dose of ThE-CsNPs on the morph/physiological characteristics of Solanum lycopersicum, as well as the severity of fusarium root rot. The relative gene expression of WRKY transcript factors and defense-associated genes were quantified in root tissues under all treatment conditions. In vitro results revealed that ThE-CsNPs (1%) had potent antifungal efficacy against F. solani radial mycelium growth. The expression of three WRKY transcription factors and three tomato defense-related genes was upregulated. Total phenolic, flavonoid content, and antioxidant enzyme activity were all increased. The outfindings of this study strongly suggested the use of ThE-CsNPs in controlling fusarium root rot on tomatoes; however, other experiments remain necessary before they are recommended

Control of meiotic recombination in plants

This project aims to locate the DNA-PKcs in Arabidopsis thaliana and identify the role by analysing Atdna-pkcs mutants. The silico, genetic and cytology data for DNA-PKCs in Arabidopsis thaliana showed that putative AtDNA-PKa and putative AtDNA-PKb are the two genes of AtDNA-PKcs in Arabidopsis thaliana. The expression data for both genes proves that AtDNA-PKCs are expressed in different tissues, but the strongly expressed in flower buds particularly in opened buds. Also, AtDNA-PKcs were expressed in male and female organs with more expression in the female organs. The data collected of double homozygous mutants suggests that Atdna-pkcs is sterile null mutant, AtDNAPKcs do not play any role in meiosis, but could be important after meiosis or in mitosis to repair DSBs through NHEJ as proves by pollen grain reduction, some anaphase bridges during mitosis, and the effect of DNA damage agents on the growth of the atdnapkcs plants. This project also aimed to study the outcome of residual DSB during meiosis in Arabidopsis thaliana. Genetic and cytology analysis was performed for Atprd2 homozygous mutant and induced DSBs by cisplatin in the absence of AtSPO11-2. The result collected demonstrates some DSB formation is observed through the presence of yH2AX foci, These DSBs are repaired by HR and producing some crossovers, and some clear rod bivalents are showed in metaphase I. However, these data suggested that partly synapsis between homologous chromosomes can produce COs through meiotic recombination machinery. Thus, study Zinc Finger Nucleases (ZFNs) has used to induce DSBs in specific sequences in the Arabidopsis thaliana genome (on TT4 and ADH1 loci) in an Atspo11.2 null mutant background. Inducible system has used to express the ZFNs and produce a specific targeted DSB during meiosis has allowed us to study the different outcomes of these DSBs. This data of induced DSBs by ZFN indicated that targeted DSB could be synopsised and repaired by meiotic recombination machinery and initiate homologous pairing and recombination in that genomic region. Nevertheless, because the sequence recognised by the ZFNs is present in both homologous chromosomes and both sister chromatids, it could produce DSBs in all these regions which would not be possible to be processed by the meiotic recombination machinery and would produce some fragmentations. This thesis will produce new insights into the control of meiotic recombination in plants

Impacts of Anthropogenic Disturbance on Vegetation Dynamics: A Case Study of Wadi Hagul, Eastern Desert, Egypt

Irresponsible human interventions, encroachment of natural habitats, and climate change negatively affect wildlife. In this study, the effects of human influence on Wadi Hagul, an unprotected area in the north of the Egyptian Eastern Desert that has recently been subjected to blatant encroachments of vegetation, were studied. The most important of these threats is the construction of the new road Al-Galala–Wadi Hagul–Zafarana. In Wadi Hagul, 80 species are reported in this study; the most represented plant families are Asteraceae (15 species) and Brassicaceae (6 species). Perennial, chamaephyte and Saharo-Arabian species were recorded in the highest percentage. Detrended canonical correspondence analysis showed that latitude, longitude, altitude, silt, sand contents, pH, and CO32− content are the factors that have the highest effect on vegetation distribution in the studied stands. Several invasive and alien species such as Euphorbia prostrata have been listed; these species typically have a negative effect on native species. The Soil Adjusted Vegetation Index (SAVI) indicated a decrease in plant cover during the study period, as compared to previous years. In 2013 and 2020, SAVI ranged from −0.02 to 0.42 and from −0.18 to 0.28, respectively. Recently, the violation and destruction of wildlife have increased, therefore, preserving it along with general biodiversity has become an urgent necessity

Green Fabrication of Zinc Oxide Nanoparticles Using Phlomis Leaf Extract: Characterization and In Vitro Evaluation of Cytotoxicity and Antibacterial Properties

Green nanoparticle synthesis is an environmentally friendly approach that uses natural solvents. It is preferred over chemical and physical techniques due to the time and energy savings. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) through a green method that used Phlomis leaf extract as an effective reducing agent. The synthesis and characterization of ZnO NPs were confirmed by UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Dynamic light scattering (DLS), Zeta potential, and Field Emission Scanning Electron Microscope (FESEM) techniques. In vitro cytotoxicity was determined in L929 normal fibroblast cells using MTT assay. The antibacterial activity of ZnO nanoparticles was investigated using a disk-diffusion method against S. aureus and E. coli, as well as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) content concentrations. XRD results confirmed the nanoparticles’ crystalline structure. Nanoparticle sizes were found to be around 79 nm by FESEM, whereas the hydrodynamic radius of nanoparticles was estimated to be around 165 ± 3 nm by DLS. FTIR spectra revealed the formation of ZnO bonding and surfactant molecule adsorption on the surface of ZnO NPs. It is interesting to observe that aqueous extracts of Phlomis leave plant are efficient reducing agents for green synthesis of ZnO NPs in vitro, with no cytotoxic effect on L929 normal cells and a significant impact on the bacteria tested

Studying Epigenetics of Cardiovascular Diseases on Chip Guide

Epigenetics is defined as the study of inheritable changes in the gene expressions and phenotypes that occurs without altering the normal DNA sequence. These changes are mainly due to an alteration in chromatin or its packaging, which changes the DNA accessibility. DNA methylation, histone modification, and noncoding or microRNAs can best explain the mechanism of epigenetics. There are various DNA methylated enzymes, histone-modifying enzymes, and microRNAs involved in the cause of various CVDs (cardiovascular diseases) such as cardiac hypertrophy, heart failure, and hypertension. Moreover, various CVD risk factors such as diabetes mellitus, hypoxia, aging, dyslipidemia, and their epigenetics are also discussed together with CVDs such as CHD (coronary heart disease) and PAH (pulmonary arterial hypertension). Furthermore, different techniques involved in epigenetic chromatin mapping are explained. Among these techniques, the ChIP-on-chip guide is explained with regard to its role in cardiac hypertrophy, a final form of heart failure. This review focuses on different epigenetic factors that are involved in causing cardiovascular diseases

Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

This study investigated the genetic diversity of bread-wheat genotypes using canopy reflectance-based vegetation indices (VIs) and simple sequence repeat (SSR) marker-based genotyping for drought tolerance. A total of 56 wheat genotypes were assessed using phenotypic traits (combination of VIs and yield traits) and 30 SSR markers. The data of the phenotypic traits were averaged over two growing seasons under irrigated and drought-stressed conditions. The hierarchical clustering of the wheat genotypes unveiled three drought-tolerant groups. Cluster 1 genotypes showed minimal phenotypic alterations, conferring superior drought tolerance and yield stability than clusters 2 and 3. The polymorphism information content values for the SSR markers ranged from 0.434 to 0.932, averaging 0.83. A total of 458 alleles (18.32 alleles per locus) were detected, with the most polymorphic markers, wmc177 and wms292, having the most alleles (24). A comparative study of SSR diversity among phenotypic clusters indicated that genotypes under cluster 1 had higher genetic diversity (0.879) and unique alleles (47%), suggesting their potential in future breeding programs. The unweighted neighbor-joining tree grouped the wheat genotypes into five major clusters. Wheat genotypes from all phenotypic clusters were distributed throughout all SSR-based clusters, indicating that genetically heterogeneous genotypes were allocated to different drought-tolerant groups. However, SSR-based clusters and model-based populations showed significant co-linearity (86.7%). The findings of the present study suggest that combining reflectance-based indirect phenotyping with SSR-based genotyping might be an effective technique for assessing genetic diversity to improve the drought tolerance of bread-wheat genotypes

Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

This study investigated the genetic diversity of bread-wheat genotypes using canopy reflectance-based vegetation indices (VIs) and simple sequence repeat (SSR) marker-based genotyping for drought tolerance. A total of 56 wheat genotypes were assessed using phenotypic traits (combination of VIs and yield traits) and 30 SSR markers. The data of the phenotypic traits were averaged over two growing seasons under irrigated and drought-stressed conditions. The hierarchical clustering of the wheat genotypes unveiled three drought-tolerant groups. Cluster 1 genotypes showed minimal phenotypic alterations, conferring superior drought tolerance and yield stability than clusters 2 and 3. The polymorphism information content values for the SSR markers ranged from 0.434 to 0.932, averaging 0.83. A total of 458 alleles (18.32 alleles per locus) were detected, with the most polymorphic markers, wmc177 and wms292, having the most alleles (24). A comparative study of SSR diversity among phenotypic clusters indicated that genotypes under cluster 1 had higher genetic diversity (0.879) and unique alleles (47%), suggesting their potential in future breeding programs. The unweighted neighbor-joining tree grouped the wheat genotypes into five major clusters. Wheat genotypes from all phenotypic clusters were distributed throughout all SSR-based clusters, indicating that genetically heterogeneous genotypes were allocated to different drought-tolerant groups. However, SSR-based clusters and model-based populations showed significant co-linearity (86.7%). The findings of the present study suggest that combining reflectance-based indirect phenotyping with SSR-based genotyping might be an effective technique for assessing genetic diversity to improve the drought tolerance of bread-wheat genotypes

Quinoa (<em>Chenopodium quinoa</em> Willd.): Genetic Diversity According to ISSR and SCoT Markers, Relative Gene Expression, and Morpho-Physiological Variation under Salinity Stress

Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic stresses, including salt. The present research examined the mechanisms of salt tolerance in five different quinoa genotypes at four different salinity levels (control (60), 80, 120, and 160 mM NaCl). ISSR and SCoT analysis revealed high polymorphism percentages of 90.91% and 85.26%, respectively. Furthermore, ISSR 1 and SCoT 7 attained the greatest number of polymorphic amplicons (27 and 26), respectively. Notably, LINE-6 and M-28 genotypes demonstrated the greatest number of unique positive and negative amplicons (50 and 42) generated from ISSR and SCoT, respectively. Protein pattern analysis detected 11 bands with a polymorphism percentage 27.27% among the quinoa genotypes, with three unique bands distinguishable for the M-28 genotype. Similarity correlation indicated that the highest similarity was between S-10 and Regeolone-3 (0.657), while the lowest similarity was between M-28 and LINE-6 (0.44). Significant variations existed among the studied salinity treatments, genotypes, and the interactions between them. The highest and lowest values for all the studied morpho-physiological and biochemical traits were recorded at 60 and 160 mM NaCl concentrations, respectively, except for the Na and proline contents, which exhibited the opposite relationship. The M-28 genotype demonstrated the highest values for all studied characteristics, while the LINE-6 genotype represented the lowest in both seasons. On the other hand, mRNA transcript levels for CqSOS1 did not exhibit differential expression in roots and leaf tissues, while the expression of CqNHX1 was upregulated more in both tissues for the M-28 genotype than for the LINE-6 genotype, and its maximum induction was seen in the leaves. Overall, the genotypes M-28 and LINE-6 were identified as the most and least salinity-tolerant, respectively

Phenotypic Plasticity in Morphological Traits of Abelmoschus esculentus L. Induced by Histone Deacetylase Inhibitor, Trichostatin A

Epigenetic changes such as DNA methylation and histone modifications, when meiotically inherited across generations, can act as a stable evolutionary force that is independent of any accompanying DNA mutations. Certain histone deacetylase (HDAC)-inhibiting chemicals such as Trichostatin A (TSA) and sodium butyrate are known to regulate the total acetylated histones in the genome, which is important for regulating the expression of various traits in all organisms. This study investigated all morphological variations in traits of Abelmoschus esculentus L. (okra) induced by different doses of Trichostatin A in a popular okra variety, Arka Abhay. Two sets of seeds were treated with two doses (0.4 &micro;M and 1.2 &micro;M) of TSA and were incubated in the chemical for three and five days, respectively, to record the effects of dose and incubation periods on various agronomic characters of okra. The treatment of TSA had a negative impact on the majority of the characters under evaluation. Total seedlings emerged, and mean shoot and root length were retarded following the TSA treatment. Extremely dwarfed plants with malformed leaves and flowers were a common observation. Pollen sterility combined with distortion of the reproductive whorls of the flowers were particularly pronounced at high doses with a prolonged incubation period. Treated plants had a significantly delayed first flowering and produced short fruits with altered morphology. Variations in seeds with respect to the number, colour and size were also recorded. Total reduction with respect to seedling parameters, total pollen production, the number of fertile pollens, plant height and other damaging effects on leaves, flowers, fruits and seeds increased as the dose and incubation period increased. Statistical analysis revealed the significant negative effect of TSA treatments on plant height, the number of ridges and locules per fruit, number of seeds per fruit and test weight. The treatment, 1.2 &micro;M Trichostatin A incubated for three days, showed a remarkable difference as traits such as total field emergence, seedling parameters and days to first flowering plant height, number of seeds per fruit and test weight deviated from the expected trend of decreasing growth parameters as the dose and incubation period increased. The study further revealed that the treatment (1.2 &micro;M TSA incubated for three days) can be suggested for use in okra to induce epigenetic variations without significantly compromising the growth and vigour parameters of okra

Active Bionanocomposite Coating Quality Assessments of Some Cucumber Properties with Some Diverse Applications during Storage Condition by Chitosan, Nano Titanium Oxide Crystals, and Sodium Tripolyphosphate

Cucumbers have a short shelf-life of about 14 days, they are perishable due to the high moisture content. This study aimed to study the effects of nano-coating material such as titanium nanoparticles and chitosan with the addition of sodium tripolyphosphate as a crosslinker to enhance cucumber quality during storage. Some essential physical, chemical, and biological parameters were determined. CH-Nano-ST (chitosan/nano titanium oxide crystals/sodium tripolyphosphate) retained the maximum greenness, −7.99, compared to CH-Nano samples, which recorded −7.31. CH-Nano (chitosan/nano titanium oxide crystals) remained the lightest, 44.38, and CH-Nano-ST was a little darker (43.73) compared to the others treatments. The discoloration was extra severe with control (22.30), which started to spoil after the end of the first week. After 21 days of the storage period at 10 °C, the reducing sugars content reduced to reach −0.64 g/100 g and −0.21 g/100 g for CH-Nano and CH-Nano-ST treatments, respectively. The CH-Nano-ST treatment presented a lower value of toughness, followed by CH-Nano at the end of the storage period. Moreover, the highest crispness index was detected for CH-Nano (5.12%), while CH-Nano-ST treatment had a slight decline to reach 4.92%. The biological results indicated that CH-Nano-ST treatment can be applied to delay the microbial contamination of Salmonella spp. in cucumbers as it reached 0.94 log CFU/g, while the CH-Nano treatment reached 1.09 log CFU/g, at the end of the storage period. In summary, nano-coating treatments with the addition of sodium tripolyphosphate can be applied to regulator postharvest quality measurements of the biological activities in cucumbers during storage at 10 °C until 21 days