Modelling the net environmental and economic impacts of urban nature-based solutions by combining ecosystem services, system dynamics and life cycle thinking: An application to urban forests

Nature-based solutions (NBS) are gaining relevance as sustainable urban actions because of their potential to provide multiple benefits in the form of ecosystem services (ES), and thus mitigate urban challenges. This paper presents an original semi-dynamic modelling framework that simultaneously considers i) ES supply and demand dynamics, ii) negative environmental impacts, externalities, and financial costs derived from NBS, and iii) life cycle NBS impacts beyond the use phase. Compared to other models, it also aims to be valuable for urban planning actions at site level, i.e., for evaluating the net impacts of specific urban NBS projects. To validate the modelling framework, a proof-of-concept model for urban forests is developed and tested for a case study in Madrid (Spain). The modelling framework is split in two interrelated parts: foreground (dynamic modelling) and background (static modelling). In the foreground, the environmental impacts derived from the use phase of an NBS project are quantified considering its spatio-temporal dynamism, by making use of system dynamics. In the background, the environmental impacts derived from the rest of the life cycle phases of the NBS are quantified making use of steady state life cycle impact assessment. The net economic impact of the NBS project, considering both financial values and externalities, is eventually calculated in the background encompassing all the life cycle phases. Results from the case study illustrate how planning, design, and management decisions over the entire life cycle of an urban forest can influence the net environmental and economic performance of this type of NBS. A discussion is provided to inform on how the modelling framework can help moving beyond the state-of-the-art, and how the derived model can be used for sustainability assessments of urban NBS projects

LaeA and VeA are involved in growth morphology, asexual development, and mycotoxin production in Alternaria alternata

Alternaria alternata is a common filamentous fungus that contaminates various fruits, grains and vegetables causing important economic losses to farmers and the food industry. A. alternata is a mycotoxigenic mould, which may jeopardize human and animal health. Two of the most common A. alternata mycotoxins found in food and feed are alternariol and alternariol monomethyl ether. In this study we examined the role of LaeA and VeA, two regulatory proteins belonging to the velvet family, which have been described to be involved in several functions in many fungi including secondary metabolism. We found that deletion of laeA and veA genes, respectively, greatly reduced sporulation and strongly compromised mycotoxin production, both in vitro or during pathogenesis of tomato fruits. We have also studied how the loss of laeA and veA may affect expression of genes related to alternariol and alternariol monomethyl ether biosynthesis (pksJ and altR), and to melanin biosynthesis (cmrA, pksA).We are also grateful to the Catalonian Government (XaRTA-Reference Network on Food Technology) for their financial support. N. Estiarte thanks the Secretaria de Universitats i Recerca del Departament d'Economia i Coneixement of the Generalitat de Catalunya for the pre-doctoral grant (FI-DGR 2013)

Determinación de residuos de plaguicidas en aceitunas empleando análisis por extracción líquida de la superficie seguida por cromatografía líquida / espectrometría de masas en tándem

Nowadays, pesticides are essential in modern agriculture for crop protection, however, this use supposes a potential risk for human health and the environment. Traditional techniques of pesticide determination require the use of laborious and complex extraction methods to separate pesticides from the matrix, above all in fatty matrices like olives. For this reason, a new simple, rapid, cheap and selective method for the extraction and quantification of the most frequently used pesticides in olive growing has been developed. Pesticide determination was carried out by ultra-performance liquid chromatography (UPLC) coupled with triple-quadrupole tandem mass spectrometry (MS/MS). Mean recoveries were found in a range between 73 and 114% with relative standard deviations lower than 20% in most pesticides evaluated and the limits of detection (LODs) and quantification (LOQs) were lower than 4 μg· kg-1 and 8 μg· kg-1, respectively. Finally, this method was applied to the analysis of 25 olive samples where Dimethoate and Terbuthylazine were detected in some cases, but their results were lower than 15 μg· kg-1.Hoy en día los pesticidas son esenciales en la agricultura moderna para la protección de los cultivos pero su uso supone un riesgo para la salud y el medio ambiente. Las técnicas tradicionales de determinación de pesticidas requieren el uso de métodos de extracción complejos a fin de separar los pesticidas de la matriz, sobre todo en matrices grasas como las aceitunas. Por ello, se ha desarrollado un nuevo método simple, rápido, barato y selectivo para la extracción y cuantificación de los pesticidas más frecuentemente utilizados en el cultivo del olivo, empleando cromatografía líquida de ultra-resolución (UPLC) acoplada a espectrometría de masas (MS/MS). Las recuperaciones alcanzadas variaron entre el 73 y 114% obteniendo desviaciones estándar relativas inferiores al 20%. Los límites de detección (LD) y cuantificación (LQ) fueron inferiores a 4 y 8 μg·kg −1, respectivamente. Finalmente, este método fue aplicado en 25 muestras de aceitunas donde se detectaron Dimetoato y Terbutilazina en algunos casos pero con valores inferiores a 15 μg·kg−1

Radiation-Induced Leiomyosarcoma after Breast Cancer Treatment and TRAM Flap Reconstruction

The development of a radiation-induced sarcoma (RIS) in the post mastectomy thoracic treatment volume is an infrequent, but recognized, event. Its frequency is rising in relation with increasing survival of breast cancer patients treated with adjuvant radiation therapy, and is associated with poor prognosis despite treatment. We present a case of leiomyosarcoma in a patient who underwent mastectomy followed by radiotherapy for invasive ductal carcinoma. A delayed TRAM flap reconstruction was performed 10 years after and a rapid growing mass under the reconstructed flap appeared, on routine follow-up, twenty years later. This report analyzes the diagnostic and therapeutic approach of patients with RIS

Calculating the energy spectra of magnetic molecules: application of real- and spin-space symmetries

The determination of the energy spectra of small spin systems as for instance given by magnetic molecules is a demanding numerical problem. In this work we review numerical approaches to diagonalize the Heisenberg Hamiltonian that employ symmetries; in particular we focus on the spin-rotational symmetry SU(2) in combination with point-group symmetries. With these methods one is able to block-diagonalize the Hamiltonian and thus to treat spin systems of unprecedented size. In addition it provides a spectroscopic labeling by irreducible representations that is helpful when interpreting transitions induced by Electron Paramagnetic Resonance (EPR), Nuclear Magnetic Resonance (NMR) or Inelastic Neutron Scattering (INS). It is our aim to provide the reader with detailed knowledge on how to set up such a diagonalization scheme.Comment: 29 pages, many figure

Urban Ecosystem accounts following the SEEA EA standard: A pilot application in Europe

National and local authorities are promoting restoration actions in urban areas to mitigate societal challenges such as urban heat island, poor air quality or biodiversity loss. Urban re-greening is among the implementation actions supporting targets of the European Green Deal, EU Biodiversity Strategy 2030, its proposal for a Nature Restoration Law, and the proposal for an amendment of the Regulation on Environmental Accounts. However, to monitor progress towards policy targets and an overall enhancement of urban ecosystems, policy makers require regular, consistent and comparable data. The implementation of United Nation's System of Environmental Economic Accounting - Ecosystem Accounting (SEEA EA) on urban ecosystems could help to track changes in their ecosystem extent, condition, services and derived benefits. Despite SEEA EA became a statistical standard, it has been only tested in pilot exercises, of which very few are urban ecosystem accounts. This report presents a pilot SEEA EA urban ecosystem account for EU-27 and EFTA Member States in 2018. It discusses challenges for the development of urban ecosystem accounts and potential solutions. The outputs illustrate where re-greening efforts should be applied and discusses feasibility and potential issues of targets. The report also presents key insights to operationalise SEEA EA for urban ecosystem accounts. It provides an instructive guiding example to national and local authorities starting to draft their own urban ecosystem accounts

Q-dependence of the inelastic neutron scattering cross section for molecular spin clusters with high molecular symmetry

For powder samples of polynuclear metal complexes the dependence of the inelastic neutron scattering intensity on the momentum transfer Q is known to be described by a combination of so called interference terms. They reflect the interplay between the geometrical structure of the compound and the spatial properties of the wave functions involved in the transition. In this work, it is shown that the Q-dependence is strongly interrelated with the molecular symmetry of molecular nanomagnets, and, if the molecular symmetry is high enough, is actually completely determined by it. A general formalism connecting spatial symmetry and interference terms is developed. The arguments are detailed for cyclic spin clusters, as experimentally realized by e.g. the octanuclear molecular wheel Cr8, and the star like tetranuclear cluster Fe4.Comment: 8 pages, 1 figures, REVTEX

Gene expression network analysis reveals new transcriptional regulators as novel factors in human ischemic cardiomyopathy

BACKGROUND: Ischemic cardiomyopathy (ICM) is characterized by transcriptomic changes that alter cellular processes leading to decreased cardiac output. Because the molecular network of ICM is largely unknown, the aim of this study was to characterize the role of new transcriptional regulators in the molecular mechanisms underlying the responses to ischemia. METHODS: Myocardial tissue explants from ICM patients and control (CNT) subjects were analyzed by RNA-Sequencing (RNA-Seq) and quantitative Real-Time PCR. RESULTS: Enrichment analysis of the ICM transcriptomic profile allowed the characterization of novel master regulators. We found that the expression of the transcriptional regulators SP100 (-1.5-fold, p < 0.05), CITED2 (-3.8-fold, p < 0.05), CEBPD (-4.9-fold, p < 0.05) and BCL3 (-3.3-fold, p < 0.05) were lower in ICM than in CNT. To gain insights into the molecular network defined by the transcription factors, we identified CEBPD, BCL3, and HIF1A target genes in the RNA-Seq datasets. We further characterized the biological processes of the target genes by gene ontology annotation. Our results suggest that CEBPD-inducible genes with roles in the inhibition of apoptosis are downregulated and that BCL3-repressible genes are involved in the regulation of cellular metabolism in ICM. Moreover, our results suggest that CITED2 downregulation causes increased expression of HIF1A target genes. Functional analysis of HIF1A target genes revealed that hypoxic and stress response genes are activated in ICM. Finally, we found a significant correlation between the mRNA levels of BCL3 and the mRNA levels of both CEBPD (r = 0.73, p < 0.001) and CITED2 (r = 0.56, p < 0.05). Interestingly, CITED2 mRNA levels are directly related to ejection fraction (EF) (r = 0.54, p < 0.05). CONCLUSIONS: Our data indicate that changes in the expression of SP100, CITED2, CEBPD, and BCL3 affect their transcription regulatory networks, which subsequently alter a number of biological processes in ICM patients. The relationship between CITED2 mRNA levels and EF emphasizes the importance of this transcription factor in ICM. Moreover, our findings identify new mechanisms used to interpret gene expression changes in ICM and provide valuable resources for further investigation of the molecular basis of human cardiac ischemic response.National Institute of Health "Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III"European Commissio