Clinical Sequencing Exploratory Research Consortium: Accelerating Evidence-Based Practice of Genomic Medicine

Despite rapid technical progress and demonstrable effectiveness for some types of diagnosis and therapy, much remains to be learned about clinical genome and exome sequencing (CGES) and its role within the practice of medicine. The Clinical Sequencing Exploratory Research (CSER) consortium includes 18 extramural research projects, one National Human Genome Research Institute (NHGRI) intramural project, and a coordinating center funded by the NHGRI and National Cancer Institute. The consortium is exploring analytic and clinical validity and utility, as well as the ethical, legal, and social implications of sequencing via multidisciplinary approaches; it has thus far recruited 5,577 participants across a spectrum of symptomatic and healthy children and adults by utilizing both germline and cancer sequencing. The CSER consortium is analyzing data and creating publically available procedures and tools related to participant preferences and consent, variant classification, disclosure and management of primary and secondary findings, health outcomes, and integration with electronic health records. Future research directions will refine measures of clinical utility of CGES in both germline and somatic testing, evaluate the use of CGES for screening in healthy individuals, explore the penetrance of pathogenic variants through extensive phenotyping, reduce discordances in public databases of genes and variants, examine social and ethnic disparities in the provision of genomics services, explore regulatory issues, and estimate the value and downstream costs of sequencing. The CSER consortium has established a shared community of research sites by using diverse approaches to pursue the evidence-based development of best practices in genomic medicine

A global experiment on motivating social distancing during the COVID-19 pandemic

Finding communication strategies that effectively motivate social distancing continues to be a global public health priority during the COVID-19 pandemic. This cross-country, preregistered experiment (n = 25,718 from 89 countries) tested hypotheses concerning generalizable positive and negative outcomes of social distancing messages that promoted personal agency and reflective choices (i.e., an autonomy-supportive message) or were restrictive and shaming (i.e., a controlling message) compared with no message at all. Results partially supported experimental hypotheses in that the controlling message increased controlled motivation (a poorly internalized form of motivation relying on shame, guilt, and fear of social consequences) relative to no message. On the other hand, the autonomy-supportive message lowered feelings of defiance compared with the controlling message, but the controlling message did not differ from receiving no message at all. Unexpectedly, messages did not influence autonomous motivation (a highly internalized form of motivation relying on one’s core values) or behavioral intentions. Results supported hypothesized associations between people’s existing autonomous and controlled motivations and self-reported behavioral intentions to engage in social distancing. Controlled motivation was associated with more defiance and less long-term behavioral intention to engage in social distancing, whereas autonomous motivation was associated with less defiance and more short- and long-term intentions to social distance. Overall, this work highlights the potential harm of using shaming and pressuring language in public health communication, with implications for the current and future global health challenges

Vegetation, browsing, and site factors as determinants of Canada yew (Taxus canadensis) distribution in central New Hampshire

Volume: 97Start Page: 357End Page: 37

Forty-Two Years of Succession Following Strip Clearcutting in a Northern Hardwoods Forest in Northwestern Massachusetts

We investigated the effects of strip width, slope position, and soil scarification in a split–split plot design on the regeneration of northern hardwoods in northwestern Massachusetts. Whole plots of 20 and 40 m in width were cut in 1954 in a second growth forest dominated by Betula papyrifera. Slope position and soil scarification were the split and split–split plot treatments, respectively. We measured height for all tree species present in randomly located 4 m2 plots beginning in 1955 and at irregular intervals over the following 42-year period. We measured all trees in the cut strips in 1996. Prunus pensylvanica was the dominant species initially, but had nearly disappeared from the cut strips by 1996. Soil scarification significantly increased initial establishment of B. papyrifera, but density and basal area of this species did not differ by soil treatment in 1996. Tree composition in cut strips was weakly correlated with soil moisture, soil scarification, and initial tree density immediately following cutting, but high spatial variation in species composition and low replication made it difficult to detect any significant correlations among the distribution and abundance of different species and selected environmental variables. The canopy of the cut strips is even-aged; establishment of most canopy trees occurred within 5 years following cutting. A comparison of successional trends in adjacent uncut strips with the trends in the cut strips indicates that cutting has altered the sequence of successional changes in forest composition increasing the abundance of some species that were of low importance prior to cutting. In 1996, Acer rubrum and A. saccharum are replacing B. papyrifera in the canopy of the uncut strips. The canopy of the cut strips consists of a diverse and spatially varying mixture of intermediate hardwoods including Quercus rubra, Fraxinus americana, Betula lenta, Acer rubrum,B. papyrifera, and an understory of late successional hardwoods

Forty-Two Years of Succession Following Strip Clearcutting in a Northern Hardwoods Forest in Northwestern Massachusetts

We investigated the effects of strip width, slope position, and soil scarification in a split–split plot design on the regeneration of northern hardwoods in northwestern Massachusetts. Whole plots of 20 and 40 m in width were cut in 1954 in a second growth forest dominated by Betula papyrifera. Slope position and soil scarification were the split and split–split plot treatments, respectively. We measured height for all tree species present in randomly located 4 m2 plots beginning in 1955 and at irregular intervals over the following 42-year period. We measured all trees in the cut strips in 1996. Prunus pensylvanica was the dominant species initially, but had nearly disappeared from the cut strips by 1996. Soil scarification significantly increased initial establishment of B. papyrifera, but density and basal area of this species did not differ by soil treatment in 1996. Tree composition in cut strips was weakly correlated with soil moisture, soil scarification, and initial tree density immediately following cutting, but high spatial variation in species composition and low replication made it difficult to detect any significant correlations among the distribution and abundance of different species and selected environmental variables. The canopy of the cut strips is even-aged; establishment of most canopy trees occurred within 5 years following cutting. A comparison of successional trends in adjacent uncut strips with the trends in the cut strips indicates that cutting has altered the sequence of successional changes in forest composition increasing the abundance of some species that were of low importance prior to cutting. In 1996, Acer rubrum and A. saccharum are replacing B. papyrifera in the canopy of the uncut strips. The canopy of the cut strips consists of a diverse and spatially varying mixture of intermediate hardwoods including Quercus rubra, Fraxinus americana, Betula lenta, Acer rubrum,B. papyrifera, and an understory of late successional hardwoods

Appendix A. Golden Eagles and lead poisoning literature summary.

Golden Eagles and lead poisoning literature summary

Appendix B. Expert elicitation methods and results.

Expert elicitation methods and results