Is your extra X chromosome holding you back? An insight into female education and academic careers in STEMM

This review discusses whether gender inequality still exists within medical, scientific and engineering academia, with regards to the career development of academic staff. In the 1970s it was suggested that women who are talented and educated with family responsibilities tend to come across problems of self-confidence and identity when attempting to enhance their professional careers, and although many are successful in doing so, others find it more challenging. By the 1990s, it was indicated that the main gender inequality mechanism in academia is the commonly known fact that women’s career development in the academic hierarchy is slower than that of men. In the past 50 years, laws and attitudes of many societies, industries and countries, have changed to promote gender equality. What is the impact of these changes, does inequality still exist and what mechanisms exist to address these issues? This review looks in depth at the links between gender equality and continuing personal and professional development (CPPD), in which individuals at work are educated more about the workplace environment and their job roles and performance. The different types, requirements and success rates of CPPD within the scientific (especially medical) academic community is discussed with an emphasis on gender equality

Hearts, and the Heartless, in the Animal Kingdom

We all take our hearts for granted: the fascinating organ inside everyone that beats continuously to keep blood pumping through our bodies. Blood flow ensures that oxygen, nutrients from food, hormones, and waste products get to the correct cells. The heart is essential for keeping humans and most animals alive. Hearts are even more interesting when we examine what they do, how they look, how they work, and the similarities and differences in the hearts of species across the planet. Is a giraffe heart similar to a human heart? Which animal survives despite having no heart? Can a heart really beat over 1,500 times a minute? From dinosaurs to insects, humans to dogs, this paper looks at what is really happening on the inside, exploring the world of heart anatomy

The effects of diet on anatomy, physiology and health in the guinea pig

Given the increasing popularity of the guinea pig as a pet, it is perhaps surprising that relatively little is published as to their husbandry and dietary requirements. Indeed, a review of the literature currently available to owners, scientists and clinicians alike has found it to be scarce, highly variable, and at times overtly contradictory. This review and data collection collates the husbandry and dietary information available and discusses the variable information available and the differing practices published in the literature in relation to the guinea pig. A questionnaire was also used to gather owner-reported data on feeding regimens from both the UK and Poland in order to better understand the range of feeding habits that owners employ. Despite the lack of information available in comparison with that available for other companion and livestock animals, advances in knowledge are being made. With guinea pig numbers increasing in many countries and owners seeking husbandry and veterinary advice and interventions, it is imperative that we understand guinea pig health and welfare issues. The data and review gives novel insights into how guinea pig husbandry affects health and welfare

Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart

The expression and function of embryonic myosin heavy chain (eMYH) has not been investigated within the early developing heart. This is despite the knowledge that other structural proteins, such as alpha and beta myosin heavy chains and cardiac alpha actin, play crucial roles in atrial septal development and cardiac function. Most cases of atrial septal defects and cardiomyopathy are not associated with a known causative gene, suggesting that further analysis into candidate genes is required. Expression studies localised eMYH in the developing chick heart. eMYH knockdown was achieved using morpholinos in a temporal manner and functional studies were carried out using electrical and calcium signalling methodologies. Knockdown in the early embryo led to abnormal atrial septal development and heart enlargement. Intriguingly, action potentials of the eMYH knockdown hearts were abnormal in comparison with the alpha and beta myosin heavy chain knockdowns and controls. Although myofibrillogenesis appeared normal, in knockdown hearts the tissue integrity was affected owing to apparent focal points of myocyte loss and an increase in cell death. An expression profile of human skeletal myosin heavy chain genes suggests that human myosin heavy chain 3 is the functional homologue of the chick eMYH gene. These data provide compelling evidence that eMYH plays a crucial role in important processes in the early developing heart and, hence, is a candidate causative gene for atrial septal defects and cardiomyopathy

Discovery of os cordis in the cardiac skeleton of chimpanzees (Pan troglodytes)

© 2020, The Author(s). Cardiovascular diseases, especially idiopathic myocardial fibrosis, is one of the most significant causes of morbidity and mortality in captive great apes. This study compared the structure and morphology of 16 hearts from chimpanzees (Pan troglodytes) which were either healthy or affected by myocardial fibrosis using X-ray microtomography. In four hearts, a single, hyperdense structure was detected within the right fibrous trigone of the cardiac skeleton. High resolution scans and histopathology revealed trabecular bones in two cases, hyaline cartilage in another case and a focus of mineralised fibro-cartilaginous metaplasia with endochondral ossification in the last case. Four other animals presented with multiple foci of ectopic calcification within the walls of the great vessels. All hearts affected by marked myocardial fibrosis presented with bone or cartilage formation, and increased collagen levels in tissues adjacent to the bone/cartilage, while unaffected hearts did not present with os cordis or cartilago cordis. The presence of an os cordis has been described in some ruminants, camelids, and otters, but never in great apes. This novel research indicates that an os cordis and cartilago cordis is present in some chimpanzees, particularly those affected by myocardial fibrosis, and could influence the risk of cardiac arrhythmias and sudden death

Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma.

Osteosarcoma (OSA) is an aggressive bone malignancy. Unlike many other malignancies, OSA outcomes have not improved in recent decades. One challenge to the development of better diagnostic and therapeutic methods for OSA has been the lack of well characterized experimental model systems. Spontaneous OSA in dogs provides a good model for the disease seen in people and also remains an important veterinary clinical challenge. We recently used RNA sequencing and qRT-PCR to provide a detailed molecular characterization of OSA relative to non-malignant bone in dogs. We identified differential mRNA expression of the solute carrier family 2 member 1 (SLC2A1/GLUT1), matrix metallopeptidase 3 (MMP3) and nuclear factor erythroid 2-related factor 2 (NFE2L2/NRF2) genes in canine OSA tissue in comparison to paired non-tumor tissue. Our present work characterizes protein expression of GLUT1, MMP3 and NRF2 using immunohistochemistry. As these proteins affect key processes such as Wnt activation, heme biosynthesis, glucose transport, understanding their expression and the enriched pathways and gene ontologies enables us to further understand the potential molecular pathways and mechanisms involved in OSA. This study further supports spontaneous OSA in dogs as a model system to inform the development of new methods to diagnose and treat OSA in both dogs and people

Molecular characterisation of canine osteosarcoma in high risk breeds

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Dogs develop osteosarcoma (OSA) and the disease process closely resembles that of human OSA. OSA has a poor prognosis in both species and disease-free intervals and cure rates have not improved in recent years. Gene expression in canine OSAs was compared with non-tumor tissue utilising RNA sequencing, validated by qRT-PCR and immunohistochemistry (n = 16). Polymorphic polyglutamine (polyQ) tracts in the androgen receptor (AR/NR3C4) and nuclear receptor coactivator 3 (NCOA3) genes were investigated in control and OSA patients using polymerase chain reaction (PCR), Sanger sequencing and fragment analysis (n = 1019 Rottweilers, 379 Irish Wolfhounds). Our analysis identified 1281 significantly differentially expressed genes (>2 fold change, p < 0.05), specifically 839 lower and 442 elevated gene expression in osteosarcoma (n = 3) samples relative to non-malignant (n = 4) bone. Enriched pathways and gene ontologies were identified, which provide insight into the molecular pathways implicated in canine OSA. Expression of a subset of these genes (SLC2A1, DKK3, MMP3, POSTN, RBP4, ASPN) was validated by qRTPCR and immunohistochemistry (MMP3, DKK3, SLC2A1) respectively. While little variation was found in the NCOA3 polyQ tract, greater variation was present in both polyQ tracts in the AR, but no significant associations in length were made with OSA. The data provides novel insights into the molecular mechanisms of OSA in high risk breeds. This knowledge may inform development of new prevention strategies and treatments for OSA in dogs and supports utilising spontaneous OSA in dogs to improve understanding of the disease in people

Anatomy, histology, development and functions of Ossa cordis: A review

This systematic review highlights the similarities and variations in Ossa cordis prevalence, histology and anatomical location between differing veterinary species and in humans. In addition, it also identifies associated factors such as aging and cardiovascular disease for each species in relation to functional roles and developmental mechanisms that these bone structures may play. The potential functions of Ossa cordis are presented, ranging from aiding cardiac contraction and conduction, providing cardiac structure, and protecting components of the heart, through to counteracting high mechanical stress. Furthermore, this review discusses the evidence and rationale behind the theories regarding the formation and development of Ossa cordis in different veterinary species and in people

The effects of diet on anatomy, physiology and health in the guinea pig

Given the increasing popularity of the guinea pig as a pet, it is perhaps surprising that relatively little is published as to their husbandry and dietary requirements. Indeed, a review of the literature currently available to owners, scientists and clinicians alike has found it to be scarce, highly variable, and at times overtly contradictory. This review and data collection collates the husbandry and dietary information available and discusses the variable information available and the differing practices published in the literature in relation to the guinea pig. A questionnaire was also used to gather owner-reported data on feeding regimens from both the UK and Poland in order to better understand the range of feeding habits that owners employ. Despite the lack of information available in comparison with that available for other companion and livestock animals, advances in knowledge are being made. With guinea pig numbers increasing in many countries and owners seeking husbandry and veterinary advice and interventions, it is imperative that we understand guinea pig health and welfare issues. The data and review gives novel insights into how guinea pig husbandry affects health and welfare

Immunohistochemical Investigation into Protein Expression Patterns of FOXO4, IRF8 and LEF1 in Canine Osteosarcoma.

Osteosarcoma (OSA) is the most common type of primary bone malignancy in people and dogs. Our previous molecular comparisons of canine OSA against healthy bone resulted in the identification of differentially expressed protein-expressing genes (forkhead box protein O4 (FOXO4), interferon regulatory factor 8 (IRF8), and lymphoid enhancer binding factor 1 (LEF1)). Immunohistochemistry (IHC) and H-scoring provided semi-quantitative assessment of nuclear and cytoplasmic staining alongside qualitative data to contextualise staining (n = 26 patients). FOXO4 was expressed predominantly in the cytoplasm with significantly lower nuclear H-scores. IRF8 H-scores ranged from 0 to 3 throughout the cohort in the nucleus and cytoplasm. LEF1 was expressed in all patients with significantly lower cytoplasmic staining compared to nuclear. No sex or anatomical location differences were observed. While reduced levels of FOXO4 might indicate malignancy, the weak or absent protein expression limits its primary use as diagnostic tumour marker. IRF8 and LEF1 have more potential for prognostic and diagnostic uses and facilitate further understanding of their roles within their respective molecular pathways, including Wnt/beta-catenin/LEF1 signalling and differential regulation of tumour suppressor genes. Deeper understanding of the mechanisms involved in OSA are essential contributions towards the development of novel diagnostic, prognostic, and treatment options in human and veterinary medicine contexts