DataSheet_1_Genome-wide identification of antisense lncRNAs and their association with susceptibility to Flavobacterium psychrophilum in rainbow trout.zip

Eukaryotic genomes encode long noncoding natural antisense transcripts (lncNATs) that have been increasingly recognized as regulatory members of gene expression. Recently, we identified a few antisense transcripts correlating in expression with immune-related genes. However, a systematic genome-wide analysis of lncNATs in rainbow trout is lacking. This study used 134 RNA-Seq datasets from five different projects to identify antisense transcripts. A total of 13,503 lncNATs were identified genome-wide. About 75% of lncNATs showed multiple exons compared to 36.5% of the intergenic lncRNAs. RNA-Seq datasets from resistant, control, and susceptible rainbow trout genetic lines with significant differences in survival rate following Flavobacterium psychrophilum (Fp) infection were analyzed to investigate the potential role of the lncNATs during infection. Twenty-four pairwise comparisons between the different genetic lines, infectious status, and time points revealed 581 differentially expressed (DE) lncNATs and 179 differentially used exons (DUEs). Most of the DE lncNATs strongly and positively correlated in expression with their corresponding sense transcripts across 24 RNA-Seq datasets. LncNATs complementary to genes related to immunity, muscle contraction, proteolysis, and iron/heme metabolism were DE following infection. LncNATs complementary to hemolysis-related genes were DE in the resistant fish compared to susceptible fish on day 5 post-infection, suggesting enhanced clearance of free hemoglobin (Hb) and heme and increased erythropoiesis. LncNATs complementary to hepcidin, a master negative regulator of the plasma iron concentration, were the most downregulated lncNATs on day 5 of bacterial infection in the resistant fish. Ninety-four DE lncNAT, including five complementary to hepcidin, are located within 26 QTL regions previously identified in association with bacterial cold water disease (BCWD) in rainbow trout. Collectively, lncNATs are involved in the molecular architecture of fish immunity and should be further investigated for potential applications in genomic selection and genetic manipulation in aquaculture.</p

Additional file 1 of RNA-Seq analysis of the pyloric caecum, liver, and muscle reveals molecular mechanisms regulating fillet color in rainbow trout

Additional file 1: Table S1: Differentially expressed genes (DEGs) in the pyloric caecum, liver, and muscle, and groups of DEG

MOESM2 of Assessment of parental perception of malaria vaccine in Tanzania

Additional file 2. Percentage distribution of perceived awareness and willing to use malaria vaccine. The data provided represent the statistical analysis of awareness and willing to use malaria vaccine. Willingness to use malaria vaccine was higher in both Zanzibar and Tanzania mainland, however, awareness of malaria vaccine was low in the regions, with Zanzibar had the lowest understanding of awareness of malaria vaccine

MOESM3 of Assessment of parental perception of malaria vaccine in Tanzania

Additional file 3. Percentage distribution of perceived benefits, mode of administering malaria vaccine and acceptance of proposed schedule. The data provided represent the statistical analysis of benefits, mode of administering malaria vaccine and acceptance of proposed schedule. Majority of women in both Zanzibar and Tanzania mainland understand the benefits of vaccine and they are ready to send their children for vaccination on any proposed schedule. However, women from Tanzania mainland accept the mode of administration (2-3 jabs) more than women in Zanzibar

MOESM1 of Assessment of parental perception of malaria vaccine in Tanzania

Additional file 1. Tool used to collect information on women’s behavioural aspects related to vaccine and malaria Vaccine. The data provided used for analysis of study on “Assessment of parental perception of malaria vaccine in Tanzania: A Case Study”

Additional file 1: of Identification of SNPs associated with muscle yield and quality traits using allelic-imbalance analyses of pooled RNA-Seq samples in rainbow trout

Putative SNPs and SNPs with allelic imbalances in association with total body weight, muscle yield, muscle fat content, shear force, and whiteness. SNP chromosome position, alleles, functional classification, associated gene ID and FASTA sequences are provided. (CSV 15332 kb

Age and frailty are independently associated with increased COVID-19 mortality and increased care needs in survivors: results of an international multi-centre study

Introduction: Increased mortality has been demonstrated in older adults with coronavirus disease 2019 (COVID-19), but the effect of frailty has been unclear. Methods: This multi-centre cohort study involved patients aged 18 years and older hospitalised with COVID-19, using routinely collected data. We used Cox regression analysis to assess the impact of age, frailty and delirium on the risk of inpatient mortality, adjusting for sex, illness severity, inflammation and co-morbidities. We used ordinal logistic regression analysis to assess the impact of age, Clinical Frailty Scale (CFS) and delirium on risk of increased care requirements on discharge, adjusting for the same variables. Results: Data from 5,711 patients from 55 hospitals in 12 countries were included (median age 74, interquartile range [IQR] 54–83; 55.2% male). The risk of death increased independently with increasing age (>80 versus 18–49: hazard ratio [HR] 3.57, confidence interval [CI] 2.54–5.02), frailty (CFS 8 versus 1–3: HR 3.03, CI 2.29–4.00) inflammation, renal disease, cardiovascular disease and cancer, but not delirium. Age, frailty (CFS 7 versus 1–3: odds ratio 7.00, CI 5.27–9.32), delirium, dementia and mental health diagnoses were all associated with increased risk of higher care needs on discharge. The likelihood of adverse outcomes increased across all grades of CFS from 4 to 9. Conclusion: Age and frailty are independently associated with adverse outcomes in COVID-19. Risk of increased care needs was also increased in survivors of COVID-19 with frailty or older age.</p