Electrostatic surface view of PCNA from shrimp <i>Litopenaeus vannamei</i> and human.

<p>The positively charged regions are colored in blue and negatively charged regions in red. The central channel is identified as a highly positive charged hole where the double-strand DNA can slide through it.</p

Proposed interaction between LvPCNA and WSSV DNA polymerase.

<p>Lateral and frontal view of the theoretical model of the polymerase is depicted with DNA. The PIP box is shown in red. PCNA is shown as an orange ring.</p

Data reduction and refinement statistics from <i>Lv</i>PCNA structure.

<p>Values in parenthesis represent the statistics at the highest resolution bin.</p>§<p>R<i>_meas</i> is a redundancy-independent version of R<i>_symm</i>, R<i>_meas</i> = ∑<i>_h</i> √n<i>_h</i>/n<i>_h</i>−1 ∑^nh_i|Î_h−I_h,i|/∑<i>_h</i> ∑^nh_iI_h,i, where Î_h = 1/n_h ∑^nh_iI_h,i.</p

Crystal structure of <i>Litopenaues vannamei</i> PCNA.

<p>The PCNA molecule is arranged as homotrimer and each monomer is shown in different color. The most important parts for protein-protein interaction of each monomer: Interdomain Conecting Loop (IDCL), Central Loop and C-terminal are labeled.</p

Aligment of best-scoring models of PCNA-WSSV PIP box complex from docking.

<p>The model shows the final seven poses for the PIP-box peptide (cartoon) docked into the binding site of <i>Lv</i>PCNA (surface representation). Tagged residues are from PCNA and form the cavity for peptide interaction. Side chains of the consensus PIP-box residues are shown as gray lines.</p

Model of <i>Lv</i>PCNA bound to WSSV PIPbox-peptide.

<p>In all figures the peptide was shorten to the consensus sequence GQHKILYYDIE that makes contact with <i>Lv</i>PCNA. Panel A shows a LigPlot where the peptide interacts with <i>Lv</i>PCNA through polar contacts (green dotted lines) and hydrophobic interaction (). Panel B shows a cartoon of the peptide (yellow) posed on a <i>Lv</i>PCNA monomer (blue), in red are identified the three region that participate in protein-protein interaction. In panel C, a surface image of <i>Lv</i>PCNA shows the hydrophobic pocket where the WSSV PIPbox-peptide (yellow) is attached. In panel D, residues that participate in <i>Lv</i>PCNA-peptide complex are tagged, side chains of residues from IDCL, Central Loop and C- terminal are green colored and the peptide residues are yellow colored.</p