LIGURIA (Italia). Cartas náuticas (1855)

Contiene 24 cartas náuticas de las costas de Liguria, incluyendo también la rada de Niza. Presentan orografía detallada por normales, indican sondas batimétricas, veriles, fondeaderos y calidad de fondo. Señalan núcleos de población, caminos, fuertes, conventos y otros edificios aislados de la costaLa mención de responsabilidad consta en la cubierta y la firma del prólogo. La portada no contiene más que el título y el pie de imprentaDedicatoria : "A S.M. la Regia di Spagna. Per Omaggio Il Vice Amiraglio della Marina Sarda Albini"Según el prefacio, este portulano es complemento de otro publicado en 1842 por el autor sobre el MediterráneoEncuadernado en terciopelo granate con grabados en oroCopia digital. Madrid : Ministerio de Cultura. Dirección General del Libro, Archivos y Bibliotecas, 201

Therapeutic potential of the metabolic modulator phenformin in targeting the stem cell compartment in melanoma

Melanoma is the most dangerous and treatment-resistant skin cancer. Tumor resistance and recurrence are due to the persistence in the patient of aggressive cells with stem cell features, the cancer stem cells (CSC). Recent evidences have shown that CSC display a distinct metabolic profile as compared to tumor bulk population: a promising anti-tumor strategy is therefore to target specific metabolic pathways driving CSC behavior. Biguanides (metformin and phenformin) are anti-diabetic drugs able to perturb cellular metabolism and displaying anti-cancer activity. However, their ability to target the CSC compartment in melanoma is not known. Here we show that phenformin, but not metformin, strongly reduces melanoma cell viability, growth and invasion in both 2D and 3D (spheroids) models. While phenformin decreases melanoma CSC markers expression and the levels of the pro-survival factor MITF, MITF overexpression fails to prevent phenformin effects. Phenformin significantly reduces cell viability in melanoma by targeting both CSC (ALDHhigh) and non-CSC cells and by significantly reducing the number of viable cells in ALDHhighand ALDHlowderived spheroids. Consistently, phenformin reduces melanoma cell viability and growth independently from SOX2 levels. Our results show that phenformin is able to affect both CSC and non-CSC melanoma cell viability and growth and suggests its potential use as anti-cancer therapy in melanoma

In-Hospital Glucose Monitoring: Adequacy and Resource Management

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Carbon Nanotubes-Based Electrochemical Sensing for Cell Culture Monitoring

Monitoring of metabolic compounds, such as glucose and lactate, is extensively reported in literature, especially for clinical purposes. Instead, the application of such technologies for monitoring metabolites in cell cultures has not been explored. From one side, such devices can provide information to the current state-of-the-art of cell lines, particularly those which are not fully known, as stem and embryonic cells. On the other hand, those systems can pave the way to fully automation for growing cell cultures, when coupled with robots for feeding. Among different presented strategies to develop biosensors,carbon nanotubes exhibit great properties, particularly suitable for biosensing. In this work nanostructured electrodes by using multi-walled carbon nanotubes are presented for the detection of glucose and lactate. Firstly, some results from simulations are illustrated in order to foresee the behavior of carbon nanotubes depending on their orientation, when they are dispersed onto the electrode surface. Then, such developed biosensors are characterized in terms of sensitivity and detection limit, and are compared to previously published results. Finally, monitoring of a cell culture is performed and the behavior of metabolites is analyzed as biosensors validation

Co-targeting triple-negative breast cancer cells and endothelial cells by metronomic chemotherapy inhibits cell regrowth and migration via downregulation of the FAK/VEGFR2/VEGF axis and autophagy/apoptosis activation

High-dose standard-of-care chemotherapy is the only option for triple-negative breast cancer (TNBC) patients, which eventually die due to metastatic tumors. Recently, metronomic chemotherapy (mCHT) showed advantages in treating TNBCs leading us to investigate the anti-metastatic and anti-angiogenic potential of metronomic 5-Fluorouracil plus Vinorelbine (5-FU+VNR) on endothelial cells (ECs) and TNBCs in comparison to standard treatment (STD). We found that 10-fold lower doses of 5-FU+VNR given mCHT vs. STD inhibits cell proliferation and survival of ECs and TNBC cells. Both schedules strongly affect ECs migration and invasion, but in TNBC cells mCHT is significantly more effective than STD in impairing cell migration and invasion. The two treatments disrupt FAK/VEGFR/VEGF signaling in both ECs and TNBC cells. mCHT, and to a much lesser extent STD treatment, induces apoptosis in ECs, whereas it switches the route of cell death from apoptosis (as induced by STD) to autophagy in TNBC cells. mCHT-treated TNBCs-derived conditioned medium also strongly affects ECs' migration, modulates different angiogenesis-associated proteins, and hampers angiogenesis in matrix sponge in vivo. In conclusion, mCHT administration of 5-FU+VNR is more effective than STD schedule in controlling cell proliferation/survival and migration/invasion of both ECs and TNBC cells and has a strong anti-angiogenic effect. Our data suggest that the stabilization of tumor growth observed in TNBC patients treated with mCHT therapy schedule is likely due not only to direct cytotoxic effects but also to anti-metastatic and anti-angiogenic effects

2024 European Society of Hypertension clinical practice guidelines for the management of arterial hypertension

Practice Guidelines: 2024 European Society of Hypertension clinical practice guidelines for the management ofarterial hypertension. Endorsed by the European Federation of Internal Medicine (EFIM), European Renal Association (ERA), and International Society ofHypertension (ISH)</p

Global disparities in surgeons’ workloads, academic engagement and rest periods: the on-calL shIft fOr geNEral SurgeonS (LIONESS) study

: The workload of general surgeons is multifaceted, encompassing not only surgical procedures but also a myriad of other responsibilities. From April to May 2023, we conducted a CHERRIES-compliant internet-based survey analyzing clinical practice, academic engagement, and post-on-call rest. The questionnaire featured six sections with 35 questions. Statistical analysis used Chi-square tests, ANOVA, and logistic regression (SPSS® v. 28). The survey received a total of 1.046 responses (65.4%). Over 78.0% of responders came from Europe, 65.1% came from a general surgery unit; 92.8% of European and 87.5% of North American respondents were involved in research, compared to 71.7% in Africa. Europe led in publishing research studies (6.6 ± 8.6 yearly). Teaching involvement was high in North America (100%) and Africa (91.7%). Surgeons reported an average of 6.7 ± 4.9 on-call shifts per month, with European and North American surgeons experiencing 6.5 ± 4.9 and 7.8 ± 4.1 on-calls monthly, respectively. African surgeons had the highest on-call frequency (8.7 ± 6.1). Post-on-call, only 35.1% of respondents received a day off. Europeans were most likely (40%) to have a day off, while African surgeons were least likely (6.7%). On the adjusted multivariable analysis HDI (Human Development Index) (aOR 1.993) hospital capacity &gt; 400 beds (aOR 2.423), working in a specialty surgery unit (aOR 2.087), and making the on-call in-house (aOR 5.446), significantly predicted the likelihood of having a day off after an on-call shift. Our study revealed critical insights into the disparities in workload, access to research, and professional opportunities for surgeons across different continents, underscored by the HDI

LIGURIA (Italia). Cartas náuticas. 1855 (1854)

Escala hallada a partir de 10 minutos de latitud [= 4,6 cm]. Coordenadas referidas al meridiano de París (E 4°51'-E 7°41'/N 44°30'-N 43°36'). Red geográfica de 10' en 10'. Orientado con dos medias lisesIndica sondas batimétricas, veriles y fondeaderosClave hidrográfica para determinar la calidad del fondoPresenta toponimia costera y la localización de los núcleos de poblaciónTítulo con grafismos decorativosCopia digital. Madrid : Ministerio de Cultura, 201