Evaluation of Micro-RNA Expression Profiling Level as Biomarkers for Diagnosis and Gene Sequencing in Patients Suffering from Breast Cancer

Background: Human bloodstream microRNAs (miRNAs) have emerged as a promising predictive and diagnostic biomarker for a range of cancers, including breast cancer. Our objective was to look into new miRNA biomarkers for diagnosis in the serum of patients with breast cancer and track the expression levels at different stages using miRNA profiling.Methods: 53 breast cancer patients and 10 healthy controls had blood samples tested for three miRNAs. miRNAs were extracted from blood and evaluated using real-time quantitative polymerase chain reaction. After extracting genomic DNA, miRNA primer-produced PCR products were sequenced to discover point mutations that may contribute to the illness.Results: After examining miR195, miR200b, and miR331, breast cancer patients had a significantly lower miR195 level than healthy persons. In addition, miR200b expression levels were significantly lower in breast cancer patients than in healthy individuals. In advanced stages, miR331 expression was substantially higher than in healthy people.Conclusion: The findings of our study demonstrated a significant association between the expression of miRNAs and the prognosis of breast cancer. Additional research is necessary to study better the correlation between these circulating miRNAs and the stages of breast cancer.Keywords: Breast cancer; Circulating miRNAs; miR195; miR200b; miR331 RT PCR; Gene Sequencing 

Evaluation of alternative solvents in common amide coupling reactions : replacement of dichloromethane and N,N-dimethylformamide

A range of alternative solvents have been evaluated within amidation reactions employing common coupling reagents with a view to identifying suitable replacements for dichloromethane and N,N-dimethylformamid

Synthesis, X-ray structure, Hirshfeld surface analysis and antimicrobial assessment of tetranuclear s-triazine hydrazine Schiff base ligand

Funding: The Deputyship for Research and Innovation, “Ministry of Education”, King Saud University (IFKSUOR3-188-3), Saudi Arabia.The unexpected tetranuclear [Cu4(DPPT)2Cl6] complex was obtained by self-assembly of CuCl2.2H2O and (E)-2,4-di(piperidin-1-yl)-6-(2-(1-(pyridin-2-yl)ethylidene)hydrazinyl)-1,3,5-triazine, ( HDPPT ) in ethanol. In this tetranuclear [Cu4(DPPT)2Cl6] complex, the organic ligand acts as mononegative chelate bridging two crystallographically independent Cu(II) sites. The DPPT− anion acts as a bidentate ligand with respect to Cu(1), while it is a tridentate for Cu(2). The Cu(1)N2Cl3 and Cu(2)N3Cl spheres have square pyramidal and square planar coordination geometries with some distortion, respectively. Two of the chloride ions coordinating the Cu(1) are bridging between two crystallographically related Cu(1) sites connecting two [Cu2(DPPT)Cl3] units together, leading to the tetranuclear formula [Cu4(DPPT)2Cl6]. The packing of the [Cu4(DPPT)2Cl6] complex is dominated by C-H…Cl contacts, leading to one-dimensional hydrogen-bond polymeric structure. According to Hirshfeld surface analysis of molecular packing, the non-covalent interactions H…H, Cl…H, Cl…C, C…H, and N…H are the most significant. Their percentages are 52.8, 19.0, 3.2, 7.7, and 9.7%, respectively. Antimicrobial assessment showed good antifungal activity of the Cu(II) complex against A. fumigatus and C. albicans compared to Ketoconazole as positive control. Moreover, the [Cu4(DPPT)2Cl6] complex has higher activity against Gram-positive bacteria than Gentamycin as positive control. The opposite was observed when testing the tetranuclear [Cu4(DPPT)2Cl6] complex against the Gram-negative bacteria.Publisher PDFPeer reviewe

Identification and Discrimination of Mycobacterium tuberculosis Complex with Traditional and Real-Time PCR in Different Specimens in Iraq

Background: Tuberculosis (TB) is a major public health issue and a main cause of global morbidity and mortality. TB is the world's ninth leading cause of death despite the numerous treatment strategies for managing the disease. Objective: To assess the traditional method (direct smear examination and culture) against real-time PCR as pulmonary and extrapulmonary tuberculosis laboratory diagnostic techniques. Cases and methods: Samples were collected from (612) TB cases, (409) of whom were pulmonary tuberculosis (PTB) and (203) were extrapolmonary tuberculosis (EPTB). The cases were seeking care at the Specialized Chest and Respiratory Disease Center/ National Reference Laboratory for Tuberculosis (NRL) in Baghdad, during the period 1st of May -1st of October 2019. Direct smear examination, Lowenstein-Jensen culture and Real Time PCR were used to diagnose TB. Results: Out of 612 samples received, 82(13.4%) were positive by smear microscopy, while 90(14.7%) were able to grow on Lowenstein-Jensen (LJ) media. Ninety DNA extracts from the samples which were positive on LJ media and 25 control specimens, were diagnosed with molecular analysis by using real time PCR to determine the species of Mycobacterium tuberculosis complex. The results revealed that the 71 samples (78.9%) were M. tuberculosis, three specimens (3.3%) were combined M. bovis and M. tuberculosis, and one M. tuberculosis, M. bovis, and M. bovis BCG, while15 (16.7%) were negative and subsequently excluded from study. Conclusion: The comparison between molecular diagnostic methods by using Real time PCR with conventional diagnostic methods, provides a new promising technique and is potentially a practical and rapid alternative to the slower traditional pulmonary and EPTB diagnostic culture. The results show M. bovis overall contribution on human TB in comparison to M. tuberculosis is minor among PTB and EPTB cases in the sample studied

HLA Profile in Iraqi Rheumatic Valvulitis Patients

Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region influence susceptibility to certain disease. Objectives: This study was established to shed light on the possible association of HLA class I and II antigens with RV patients. Patients and Methods: Lymphocytotoxicity assay for HLA for class I and II typing had been done for (100) Iraqi patients suffering from rheumatic valvulitis (RV), the control groups consisting of (75 healthy individuals and 35 non rheumatic heart disease (NRHD) patients ). Results: The results showed a significant association of A33-Ags with these patients as compared with healthy and cardiac controls (P=0.005), (P=0.033) respectively. Another interesting finding was the low frequency of A1 in RV patients when compared with healthy control (p=0.002), suggesting that A1 allele may confer protective effect against this disease. In addition significant association between blood group B and RV was evident (p=0.04). An interesting observation was a strong association of blood group B and A33 among those patients (P<0.001). Conclusion: The present results are consistent with hypothesis that susceptibility to RV is genetically linked and in turn may be associated mainly with A33 in Iraqi patients

Enhancement flame flashback resistance against CIVB and BLF in swirl burners

Swirl combustors have proven as effective flame stabilisers over a wide range of operation conditions thanks to the formation of well-known swirl coherent structures. However, employment of swirl combustors to work on lean premixed combustion modes while introducing alternative fuels such as high hydrogen blends result in many combustion instabilities. Under these conditions, flame flashback has been considered as one of the major instability problems that have the potential of causing considerable damages of the combustion systems hardware in addition to the significant increase in pollutant levels. Combustion Induced Vortex Breakdown (CIVB) is considered a very particular mode of flashback mechanism in swirling flows as this type of flashback occurs even when the fresh mixture’s velocity is higher than the flame speed, consequence of the interaction between swirl structures and swirl burner geometries. Improvements of burner geometries and manipulation of swirl flows can produce good resistance against this type of flashback. However, increase flame flashback resistance against CIVB can lead to an increase in the propensity of another flashback mechanism, Boundary Layer Flashback (BLF). Thus this paper presents an experimental and numerical approach that allows the increase in CIVB resistance by using diffusive air injection and simultaneously avoid BLF by changing the wall boundary layer characteristics using microsurface grids as a liner for the nozzle wall. Results show that using those two techniques together has promising potentials regarding wider stable operation for swirl combustors, enabling them to burn a great variety of fuel blends safely

Enhancing flame flashback resistance against Combustion Induced Vortex Breakdown and Boundary Layer Flashback in swirl burners

Swirl combustors have proven to be effective flame stabilisers over a wide range of operation conditions thanks to the formation of well-known swirl coherent structures. However, their employment for lean premixed combustion modes while introducing alternative fuels such as high hydrogenated blends results in many combustion instabilities. Under these conditions, flame flashback is considered one of the major instability problems that have the potential of causing considerable damage to combustion systems hardware in addition to the significant increase in pollutant levels. Combustion Induced Vortex Breakdown is considered a very particular mode of flashback instability in swirling flows as this type of flashback occurs even when the fresh mixture velocity is higher than the flame speed, a consequence of the interaction between swirl structures and swirl burner geometries. Improvements in burner geometries and manipulation of swirling flows can increase resistance against this type of flashback. However, increasing resistance against Combustion Induced Vortex Breakdown can lead to augmentation in the propensity of another flashback mechanism, Boundary Layer Flashback. Thus, this paper presents an experimental approach of a combination of techniques that increase Combustion Induced Vortex Breakdown resistance, i.e. by repositioning a central injector and using central air injection, while simultaneously avoiding Boundary Layer Flashback, i.e. by changing the wall boundary layer characteristics using microsurfaces on the nozzle wall. Results show that using these techniques together has promising potentials regarding wider stable operation for swirl combustors, enabling them to burn a broader variety of fuel blends safely, while informing developers of the improvements obtained with the combined techniques

Community pharmacists’ knowledge, attitude, and practices towards dispensing antibiotics without prescription (DAwP): a cross-sectional survey in Makkah Province, Saudi Arabia

Objectives: To evaluate knowledge, attitude, and practices of community pharmacists towards dispensing antibiotics without prescription (DAwP) in Makkah Province, Saudi Arabia. Methods: A cross-sectional survey was conducted between January and February 2016 using a structured, validated, and pilot-tested questionnaire. A four-step systematic approach was used to recruit community pharmacists who completed a 28-item questionnaire either in English or Arabic language based on their personal preference. Results: Of the 200 community pharmacists approached, 189 completed the questionnaire. More than two-thirds (70.5%) of the pharmacists were not aware that DAwP is illegal practice. Lack of patient willingness to consult a physician for a non-serious infection (69.9%) and an inability to afford a consultation with a physician (65.3%) were the most common reasons cited for DAwP. A statistically significant association was found between the number of antibiotics dispensed and educating patients about the importance of adherence and completion of the full course of antibiotics (p = 0.007). Conclusions: In general, community pharmacists have a poor understanding of the regulations prohibiting the over-the-counter sale of antibiotics in Saudi Arabia, explaining the high rate of DAwP in the country. A multifaceted approach consisting of educational interventions and improving the access to and affordability of healthcare facilities for the general public is required to effectively reduce DAwP and its negative consequences on public health

VLPs and particle strategies for cancer vaccines

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First Dark Matter Search Results from the LUX-ZEPLIN (LZ) Experiment

The LUX-ZEPLIN experiment is a dark matter detector centered on a dual-phase xenon time projection chamber operating at the Sanford Underground Research Facility in Lead, South Dakota, USA. This Letter reports results from LUX-ZEPLIN's first search for weakly interacting massive particles (WIMPs) with an exposure of 60 live days using a fiducial mass of 5.5 t. A profile-likelihood ratio analysis shows the data to be consistent with a background-only hypothesis, setting new limits on spin-independent WIMP-nucleon, spin-dependent WIMP-neutron, and spin-dependent WIMP-proton cross sections for WIMP masses above 9 GeV/c2. The most stringent limit is set for spin-independent scattering at 36 GeV/c2, rejecting cross sections above 9.2×10-48 cm at the 90% confidence level