Four-Day Modified School Systems in Rural Oklahoma

Over the past 40 years, school districts in rural areas have been forced to move to a modified four-day school schedule. As of 2019, 650 schools in over 25 states operate on a four-day modified school model. The purpose of this qualitative case study was to explore teachers’ and administrators’ perceptions of the four-day school week. Four research questions guided the study: (a) How do teachers and administrators perceive the four-day school system in Oklahoma?, (b) Why do teachers and administrators support or not support a four-day school system in Oklahoma?, (c) How do teachers and administrators perceive the impact of the four- day school system on students’ academic performance?, and (d) What suggestions do teachers and administrators have for districts considering the implementation of the four-day school week? The participants were 15 teachers and five administrators from rural school districts in Oklahoma. Interviews and a focus group discussion were adopted as instruments for the study. Data were collected through Zoom and analyzed manually. The findings showed seven significant emergent themes. The significant themes for interviews were (a) increased teacher and student attendance, (b) increased student morale and decreased discipline issues, (c) increased teacher morale and retention, and (d) more time for family and personal business. The significant themes for the focus group discussion consisted of (a) increased teacher and student attendance, (b) increased student morale and decreased discipline issues, and (c) school finance benefits. Recommendations were included

Cyclic di-GMP is Essential for the Survival of the Lyme Disease Spirochete in Ticks

Cyclic dimeric GMP (c-di-GMP) is a bacterial second messenger that modulates many biological processes. Although its role in bacterial pathogenesis during mammalian infection has been documented, the role of c-di-GMP in a pathogen's life cycle within a vector host is less understood. The enzootic cycle of the Lyme disease pathogen Borrelia burgdorferi involves both a mammalian host and an Ixodes tick vector. The B. burgdorferi genome encodes a single copy of the diguanylate cyclase gene (rrp1), which is responsible for c-di-GMP synthesis. To determine the role of c-di-GMP in the life cycle of B. burgdorferi, an Rrp1-deficient B. burgdorferi strain was generated. The rrp1 mutant remains infectious in the mammalian host but cannot survive in the tick vector. Microarray analyses revealed that expression of a four-gene operon involved in glycerol transport and metabolism, bb0240-bb0243, was significantly downregulated by abrogation of Rrp1. In vitro, the rrp1 mutant is impaired in growth in the media containing glycerol as the carbon source (BSK-glycerol). To determine the contribution of the glycerol metabolic pathway to the rrp1 mutant phenotype, a glp mutant, in which the entire bb0240-bb0243 operon is not expressed, was generated. Similar to the rrp1 mutant, the glp mutant has a growth defect in BSK-glycerol medium. In vivo, the glp mutant is also infectious in mice but has reduced survival in ticks. Constitutive expression of the bb0240-bb0243 operon in the rrp1 mutant fully rescues the growth defect in BSK-glycerol medium and partially restores survival of the rrp1 mutant in ticks. Thus, c-di-GMP appears to govern a catabolic switch in B. burgdorferi and plays a vital role in the tick part of the spirochetal enzootic cycle. This work provides the first evidence that c-di-GMP is essential for a pathogen's survival in its vector host