Utjecaj toplinskog stresa na kakvoću jaja nesilica hranjenih smjesom s dodatkom laskorbinske kiseline i l-tokoferol acetata.

The aim of the experiment was to investigate the effects of heat stress on egg quality profi le in layer hens supplemented with vitamins C and E. A total of 720 L33 layer hens at 39 weeks old, were divided into four groups of 180 birds. One group was fed a basal diet (control) and treatment groups were fed a basal diet supplemented with 150 mg of l-ascorbic acid/kg of diet (Vit. C group), 150 mg of α-dl-tocopherol acetate/kg of diet (Vit. E group), while the last group was supplemented with 150 mg of l-ascorbic acid/kg of diet plus 150 mg of α-dl-tocopherol acetate/kg of diet (Vit C + E group). Egg, albumen and eggshell weights were higher (P<0.05) and (P<0.005) in vitamins E and C + E groups when compared to vitamin C and control groups. Egg yolk was higher (P<0.05), (P<0.005) and (P<0.0005) in the vitamin C + E group, compared to the vitamin E and vitamin C treated groups and control respectively. Egg shell thickness, egg resistance and specifi c gravity showed (P<0.05) and (P<0.005) in the vitamins C + E group, compared to the vitamin C and E groups, and control. Haugh Unit was higher (P<0.05) and (P<0.005) in the vitamins C + E treated group compared to vitamin C and E treated groups, and control. The results suggest that the supplementation of antioxidant vitamins had a benefi cial effect on egg quality in heat stressed layer hens.Proveden je pokus s ciljem da se istraže učinci toplinskog stresa na kakvoću jaja nesilica kojima su u hrani dodani vitamin C i E. Ukupno je 720 nesilica L33 u dobi od 39 tjedana bilo podijeljeno u četiri skupine po 180 nesilica. Kontrolna skupina dobivala je osnovnu hranu, dok je jedna pokusna skupina dobivala osnovnu hranu s dodatkom 150 mg l-askorbinske kiseline/kg (vit. C skupina), druga 150 mg α-dl-tokoferol acetata/kg (vit. E skupina), a treća skupina dobivala je osnovnu hranu s dodatkom 150 mg l-askorbiske kiseline/kg i 150 mg α- dl-tokoferol acetata/kg (vit C+E skupina). Težine bjelanjka (P<0,05) i ljuske (P<0,005) bile su veće u skupini koja je dobivala vitamin E i skupini koja je dobivala vitamine C+E u usporedbi sa skupinom koja je dobivala samo vitamin C i kontrolnom skupinom. Žutanjak je bio teži (P<0,05) u skupini koja je dobivala vitamin C+E u usporedbi sa skupinom koja je dobivala vitamin E (P<0,005), vitamin C (P<0,0005) i kontrolnom skupinom. Razlika u debljini ljuske jajeta, čvrstoći jajeta i specifičnoj težini bila je na razini P<0,05 u skupini C+E u usporedbi sa skupinama koje su dobivale C vitamin i E vitamin (P<0,005) i kontrolnom skupinom. Haughova jedinica bila je veća (P<0,05) u skupini koja je dobivala vitamine C+E u odnosu na skupine koje su zasebno dobivale vitamin C ili E (P<0,005) i kontrolnu skupinu. Rezultati pokazuju da dodatak antioksidantnih vitamina ima povoljan učinak na kakvoću jaja u nesilica izloženih toplinskom stresu

Ultrathin 2 nm gold as ideal impedance-matched absorber for infrared light

Thermal detectors are a cornerstone of infrared (IR) and terahertz (THz) technology due to their broad spectral range. These detectors call for suitable broad spectral absorbers with minimalthermal mass. Often this is realized by plasmonic absorbers, which ensure a high absorptivity butonly for a narrow spectral band. Alternativly, a common approach is based on impedance-matching the sheet resistance of a thin metallic film to half the free-space impedance. Thereby, it is possible to achieve a wavelength-independent absorptivity of up to 50 %, depending on the dielectric properties of the underlying substrate. However, existing absorber films typicallyrequire a thickness of the order of tens of nanometers, such as titanium nitride (14 nm), whichcan significantly deteriorate the response of a thermal transducers. Here, we present the application of ultrathin gold (2 nm) on top of a 1.2 nm copper oxide seed layer as an effective IR absorber. An almost wavelength-independent and long-time stable absorptivity of 47(3) %, ranging from 2 $\mu$m to 20 $\mu$m, could be obtained and is further discussed. The presented gold thin-film represents analmost ideal impedance-matched IR absorber that allows a significant improvement of state-of-the-art thermal detector technology

Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS

The formation of acquired drug resistance is a major reason for the failure of anti-cancer therapies after initial response. Here, we introduce a novel model of acquired oxaliplatin resistance, a sub-line of the non-MYCN-amplified neuroblastoma cell line SK-N-AS that was adapted to growth in the presence of 4000 ng/mL oxaliplatin (SK-N-ASrOXALI4000). SK-N-ASrOXALI4000 cells displayed enhanced chromosomal aberrations compared to SK-N-AS, as indicated by 24-chromosome fluorescence in situ hybridisation. Moreover, SK-N-ASrOXALI4000 cells were resistant not only to oxaliplatin but also to the two other commonly used anti-cancer platinum agents cisplatin and carboplatin. SK-N-ASrOXALI4000 cells exhibited a stable resistance phenotype that was not affected by culturing the cells for 10 weeks in the absence of oxaliplatin. Interestingly, SK-N-ASrOXALI4000 cells showed no cross resistance to gemcitabine and increased sensitivity to doxorubicin and UVC radiation, alternative treatments that like platinum drugs target DNA integrity. Notably, UVC-induced DNA damage is thought to be predominantly repaired by nucleotide excision repair and nucleotide excision repair has been described as the main oxaliplatin-induced DNA damage repair system. SK-N-ASrOXALI4000 cells were also more sensitive to lysis by influenza A virus, a candidate for oncolytic therapy, than SK-N-AS cells. In conclusion, we introduce a novel oxaliplatin resistance model. The oxaliplatin resistance mechanisms in SK-N-ASrOXALI4000 cells appear to be complex and not to directly depend on enhanced DNA repair capacity. Models of oxaliplatin resistance are of particular relevance since research on platinum drugs has so far predominantly focused on cisplatin and carboplatin

Effects of heat stress on some blood parameters and egg production of Shika Brown layer chickens transported by road

The effects of vitamins C and E on layer chickens transported by road for 6 h during the hot dry season were investigated. Two experimental groups consisting of thirty Shika Brown layers were separately administered vitamins C and E orally just before transportation, while another 30 layers, which were only given sterile water, served as control. Blood samples analyzed before and after transportation in the control layers showed a decrease (p0.05) from those obtained before transportation. Heterophil/lymphocyte values were highest in the control group. The result showed that transportation was stressful for the control layers. Post-transportation egg production was not signifcantly (p>0.05) different in the vitamin E treated group, but values recorded for the vitamin C and control groups were signifcantly (p<0.05) and (p<0.001) reduced compared to pre-transportation. In conclusion, vitamins C and E administration ameliorated the adverse effect of road transportation stress during the hot dry season