The problem of offset in acoustic pulse reflectometry

Acoustic pulse reflectometry has become established as a useful non-invasive technique for measuring a variety of duct properties. A sound pulse is injected into the duct under investigation and the resultant reflections are recorded. Suitable analysis of the reflections yields the input impulse response of the duct, from which both its input impedance and its internal dimensions can be calculated. However, an input impulse response measurement made using acoustic pulse reflectometry generally contains an offset. Unless this offset is removed, the application of a bore reconstruction algorithm results in a calculated duct profile which expands or contracts spuriously. In this paper, the offset in an input impulse response measurement is shown to consist of both constant and time-varying components. Methods of preventing or removing these DC and time-varying offsets are proposed and subsequent improvements to the bore reconstruction accuracy are demonstrated

Improvements to the acoustic pulse reflectometry technique for measuring duct dimensions

In the study of tubular structures such as pipeline sections, musical wind instruments and human airways, acoustic pulse reflectometry has become established as a useful tool for non-invasively measuring the input impulse response, from which the internal duct dimensions can be calculated. In this thesis, the theory describing wave propagation in a duct of varying crosssection is outlined, culminating in a discussion of the layer peeling algorithm used to reconstruct a duct’s bore profile from its input impulse response. Experimental measurements of the input impulse responses of various test objects, together with the subsequent bore reconstructions, are then presented. The problem of offset in input impulse response measurements is discussed and the effect on the bore reconstruction is shown. The offset is found to consist of both a DC component and a sinusoidal component. Methods for eliminating the two offset components are explored and the resultant improvement in the stability and reproducibility of the bore reconstructions is demonstrated. Two adaptations to the reflectometry technique, designed to extend the bandwidth of input impulse response measurements, are described. The improved high frequency content brought about by these adaptations is shown to lead to bore reconstructions of high axial resolution, allowing rapid changes in cross-sectional area to be more accurately reproduced. Finally, limitations of the acoustic pulse reflectometry technique (particularly those brought about by the bandwidth improvements) are discussed and potential future ways of overcoming the limitations are proposed

The short-time behavior of kinetic spherical model with long-ranged interactions

The kinetic spherical model with long-ranged interactions and an arbitrary initial order m_{0} quenched from a very high temperature to T < T_{c} is solved. In the short-time regime, the bulk order increases with a power law in both the critical and phase-ordering dynamics. To the latter dynamics, a power law for the relative order m_{r} ~ -t^{-k} is found in the intermediate time-regime. The short-time scaling relation of small m_{0} are generalized to an arbitrary m_{0} and all the time larger than t_{mic}. The characteristic functions $\phi (b,m_{0})$ for the scaling of m_{0} and $\epsilon (b,T')$ for T'=T/T_{c} are obtained. The crossover between scaling regimes is discussed in detail.Comment: 22 pages, 3 figure

Auricle shaping using 3D printing and autologous diced cartilage.

ObjectiveTo reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP).MethodsMaterialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness.ResultsThe 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P &gt; 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans.ConclusionAuricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties.Levels of evidenceNA. Laryngoscope, 129:2467-2474, 2019

Alkaline protease production by solid state fermentation on polyurethane foam

This paper investigated the process of solid state fermentation (SSF) using PUF (polyurethane foam) as inert solid support to produce alkaline protease. Maximal enzyme activity was 2185U/ml at pH 9.0, incubation temperature 32 0C inoculum amount of 1.0 % (v/v) , nutrient solution3.75 ml/g PUF, incubation time for 2 h and 15.0 mM of added CaCl2. Under the same conditions, the yield of alkaline protease produced by SSF using PUF as support is higher than that by submerged fermentation (SMF)

Effects of Tonal Coarticulation and Prosodic Positions on Tonal Contours of Low Rising Tones: In the Case of Xiamen Dialect

Few studies have worked on the effects of tonal coarticulation and prosodic positions on the low rising tone in Xiamen Dialect. This study addressed such an issue. To do so, a new method, the Tonal Contour Analysis in Tonal Triangle, was proposed to measure the subtle curvature of the tonal contour. Findings are as follows: (1) The low rising tone in Xiamen Dialect has a tendency towards the falling-rising tone, which is significantly affected by the tonal coarticulation and prosodic positions. (2) The low rising tone presents as a falling-rising tone when preceded by a tone with a high offset, and as a low rising tone when preceded by a tone that ends up low. (3) The curvature of the low rising tone is greatest in the sentence-initial position, and is positively correlated to its own duration.Comment: To be published in InterSpeech 202

Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization.

Implanted biomaterials and biomedical devices generally induce foreign body reaction and end up with encapsulation by a dense avascular fibrous layer enriched in extracellular matrix. Fibroblasts/myofibroblasts are thought to be the major cell type involved in encapsulation, but it is unclear whether and how stem cells contribute to this process. Here we show, for the first time, that Sox10+ adult stem cells contribute to both encapsulation and microvessel formation. Sox10+ adult stem cells were found sparsely in the stroma of subcutaneous loose connective tissues. Upon subcutaneous biomaterial implantation, Sox10+ stem cells were activated and recruited to the biomaterial scaffold, and differentiated into fibroblasts and then myofibroblasts. This differentiation process from Sox10+ stem cells to myofibroblasts could be recapitulated in vitro. On the other hand, Sox10+ stem cells could differentiate into perivascular cells to stabilize newly formed microvessels. Sox10+ stem cells and endothelial cells in three-dimensional co-culture self-assembled into microvessels, and platelet-derived growth factor had chemotactic effect on Sox10+ stem cells. Transplanted Sox10+ stem cells differentiated into smooth muscle cells to stabilize functional microvessels. These findings demonstrate the critical role of adult stem cells in tissue remodeling and unravel the complexity of stem cell fate determination

Semantic Bimodal Presentation Differentially Slows Working Memory Retrieval

Although evidence has shown that working memory (WM) can be differentially affected by the multisensory congruency of different visual and auditory stimuli, it remains unclear whether different multisensory congruency about concrete and abstract words could impact further WM retrieval. By manipulating the attention focus toward different matching conditions of visual and auditory word characteristics in a 2-back paradigm, the present study revealed that for the characteristically incongruent condition under the auditory retrieval condition, the response to abstract words was faster than that to concrete words, indicating that auditory abstract words are not affected by visual representation, while auditory concrete words are. Alternatively, for concrete words under the visual retrieval condition, WM retrieval was faster in the characteristically incongruent condition than in the characteristically congruent condition, indicating that visual representation formed by auditory concrete words may interfere with WM retrieval of visual concrete words. The present findings demonstrated that concrete words in multisensory conditions may be too aggressively encoded with other visual representations, which would inadvertently slow WM retrieval. However, abstract words seem to suppress interference better, showing better WM performance than concrete words in the multisensory condition

Differentiation of multipotent vascular stem cells contributes to vascular diseases.

It is generally accepted that the de-differentiation of smooth muscle cells, from the contractile to the proliferative/synthetic phenotype, has an important role during vascular remodelling and diseases. Here we provide evidence that challenges this theory. We identify a new type of stem cell in the blood vessel wall, named multipotent vascular stem cells. Multipotent vascular stem cells express markers, including Sox17, Sox10 and S100β, are cloneable, have telomerase activity, and can differentiate into neural cells and mesenchymal stem cell-like cells that subsequently differentiate into smooth muscle cells. On the other hand, we perform lineage tracing with smooth muscle myosin heavy chain as a marker and find that multipotent vascular stem cells and proliferative or synthetic smooth muscle cells do not arise from the de-differentiation of mature smooth muscle cells. In response to vascular injuries, multipotent vascular stem cells, instead of smooth muscle cells, become proliferative, and differentiate into smooth muscle cells and chondrogenic cells, thus contributing to vascular remodelling and neointimal hyperplasia. These findings support a new hypothesis that the differentiation of multipotent vascular stem cells, rather than the de-differentiation of smooth muscle cells, contributes to vascular remodelling and diseases