Does supplemental zeolite (clinoptilolite) affect growth performance, meat texture, oxidative stress and production of polyunsaturated fatty acid of Turkey poults?

Abstract Background Following the ban on the use of growth factors, the use of zeolite in poultry feed could be a solution to obtain healthier food products that are more demanded by the consumer. Methods Zeolite (Clinoptilolite) was added to turkey male and female feed at concentrations 1% or 2% and was evaluated for its effectiveness on performance of the production. The turkeys were given free and continuous access to a nutritionally non-limiting diet (in meal form) that was either a basal diet or a ‘Zeolite supplemented-diet’ (the basal diet supplemented with clinoptilolite at a level of 1% or 2%). Results It was found that adding zeolite in the turkey diet had a positive effect on growth performance and increased weight gain compared to the control. In addition, zeolite treatment had a positive effect on oxidative stress and organoleptic parameters that were measured. It was found that adding zeolite in the turkey diet reduced the MDA level in the liver and in the meat, as compared to the control. Quality of meat was measured as a significantly increase (p < 0.05) in pH for male meat, indicated that the zeolite could maintain the quality of longer period. The adding of zeolite in the turkey diet increased level of polyunsaturated fatty acid. Conclusion This study showed the significance of using zeolite, as a feed additive for turkey, as part of a comprehensive program to improve growth performance and oxidative stress parameters and to increase level of polyunsaturated fatty acid on the turkey body

Protective Role of Spirulina platensis against Bifenthrin-Induced Reprotoxicity in Adult Male Mice by Reversing Expression of Altered Histological, Biochemical, and Molecular Markers Including MicroRNAs

International audienceThe potential reprotoxicity of bifenthrin remains unclear if only the common clinical indicators of reproductive disease are examined. The present study aimed to investigate the efficacy of Spirulina platensis, a microalga rich in antioxidant compounds, against bifenthrin-induced testicular oxidative damage in male mice. At the first, we demonstrate that administration of bifenthrin resulted in a decline of testosterone level and in deterioration of sperm quality that was correlated with significant transcription changes of some specific mRNA and microRNA involved in cholesterol transport, testosterone synthesis, and spermatogenesis. At the biochemical level, we found that oxidative stress was obvious in the bifenthrin group, as evidenced by increase in malondialdehyde (MDA), protein carbonyls (PCO), reactive oxygen species (ROS), and nitrite oxide (NO) that was correlated with activation of genes related to mitochondrial apoptotic signal pathways. We then brought, for the first time to our knowledge, solid and complete experimental evidences that administration of mice with Spirulina extract was sufficient to protect against deleterious effects BF in testicular tissues by abrogating the change in antioxidant enzyme activities; the increase in MDA, PCO, and NO concentrations; and the altered expression level of miRNA and mRNA involved in spermatogenesis. We finally demonstrate that Spirulina restores the production of testosterone in mice as well as epididymal sperm viability and motility. These results suggest a potential antitoxic activity of Tunisian Spirulina deserving further attention

Preventing bifenthrin-induced testicular toxicity in rats with <i>Citrus sinensis</i> (‘Maltese Half-blood’) essential oil: a multidisciplinary approach

The widespread use of pesticides, particularly bifenthrin, has raised concerns about their impact on the environment and human health. Bifenthrin has been shown to have adverse effects on male reproductive health, making it a pressing issue to address. In this study, we investigated the potential of Citrus sinensis ‘Maltese Half-Blood’ essential oil (CsEO) to mitigate bifenthrin-induced testicular toxicity. Our approach combined molecular docking, network pharmacology, histopathology, along with in vitro and in vivo assays. to evaluate the antioxidant properties of CsEO and its ability to counteract bifenthrin’s harmful effects. Our results showed that CsEO possesses robust antioxidant attributes, primarily due to its rich phytochemical composition. Limonene, α-pinene, and γ-terpinene were identified as the most contributive compounds. In rat models, CsEO treatment significantly alleviated oxidative stress markers induced by bifenthrin. Network pharmacology analyses revealed the intricate interactions between CsEO’s molecular constituents and their biological targets, with compounds like cedrene and geraniol playing key roles. Molecular docking data demonstrated that certain essential oil components have binding affinities to protein targets that rival or even exceed those of bifenthrin. CsEO effectively counteracted bifenthrin-induced testicular oxidative stress, highlighting its therapeutic potential. Our findings provide compelling evidence that CsEO can effectively counteract the harmful effects of bifenthrin on testicular health.</p

The number of samples positive for each pair of pathogens.

<p>The number of samples positive for each pair of pathogens.</p

Sequencing results of vaginal swab samples positive with the pan-<i>Chlamydiales</i> PCR.

<p>*These results were previously published by Barkallah et al. (2013).</p

The association of abortion with positive results for different agents and frequencies of positive results between vaginal and milk samples.

<p>The association of abortion with positive results for different agents by real-time PCR and frequencies of positive results between vaginal and milk samples were assessed using the Fisher's exact test. A p value<0.05 was taken as a level of significance. Cases: cows with clinical signs, controls: cows with normal pregnancies and normal parturitions, OR: odds ratio, CI: confidence interval.</p

Details of cases positive for <i>Brucella abortus</i>.

<p>+ positive/ − negative.</p

Sequences of primers and probes used in the study.

<p>Sequences of primers and probes used in the study.</p

Correlation between real-time PCR and the RBPT results.

<p>The mean Ct-value of samples negative for <i>Brucella</i> (5.95) was significantly lower than that of samples with a strong reaction results (24.71), which is lower than that of samples with a weak reaction (34.61) (p<0,0001).</p