Assessment and Characterization of Irrigation Quality of Ground Water in Bahawalpur District, Pakistan.

Ground water development has contributed significantly to food security and reduction in poverty in Pakistan. Due to rapid population growth, there has been a dramatic increase in the intensity of ground water exploitation leading to declining water table and deteriorating ground water quality. In such prevailing conditions, hydro geological appraisal of escalating ground water exploitation has become a paramount importance. Keeping in this view, a study was undertaken to categorize suitability of ground water in the area of Bahawalpur district for irrigation purpose.  Total 19207 water samples were collected from all the five tehsils of district Bahawalpur during the year July 2011 to June 2013. These samples were analyzed and categorized according to suitability criteria of water quality evaluation. 39 percent water samples were found fit, 12 percent were marginally fit and 49 percent water samples were found unfit for irrigation purpose. Majority of water samples were found hazardous for irrigation purpose. The soils under study were heavy texture, medium alkaline, low to medium in P and high in K. It is suggested that there is urgent need to evaluate the effects of tube well water on crop yields. Keywords: EC, SAR, RSC, Ground water, Bahawalpur, Pakistan

Nucleotide identity and variability among different Pakistani hepatitis C virus isolates

<p>Abstract</p> <p>Background</p> <p>The variability within the hepatitis C virus (HCV) genome has formed the basis for several genotyping methods and used widely for HCV genotyping worldwide.</p> <p>Aim</p> <p>The aim of the present study was to determine percent nucleotide identity and variability in HCV isolates prevalent in different geographical regions of Pakistan.</p> <p>Methods</p> <p>Sequencing analysis of the 5'noncoding region (5'-NCR) of 100 HCV RNA-positive patients representing all the four provinces of Pakistan were carried out using ABI PRISM 3100 Genetic Analyzer.</p> <p>Results</p> <p>The results showed that type 3 is the predominant genotypes circulating in Pakistan, with an overall prevalence of 50%. Types 1 and 4 viruses were 9% and 6% respectively. The overall nucleotide similarity among different Pakistani isolates was 92.50% ± 0.50%. Pakistani isolates from different areas showed 7.5% ± 0.50% nucleotide variability in 5'NCR region. The percent nucleotide identity (PNI) was 98.11% ± 0.50% within Pakistani type 1 sequences, 98.10% ± 0.60% for type 3 sequences, and 99.80% ± 0.20% for type 4 sequences. The PNI between different genotypes was 93.90% ± 0.20% for type 1 and type 3, 94.80% ± 0.12% for type 1 and type 4, and 94.40% ± 0.22% for type 3 and type 4.</p> <p>Conclusion</p> <p>Genotype 3 is the most prevalent HCV genotype in Pakistan. Minimum and maximum percent nucleotide divergences were noted between genotype 1 and 4 and 1 and 3 respectively.</p

Sustainable Irrigation Management for Higher Yield

Sustainable irrigation is sensible application of watering to plants in agriculture, landscapes that aids in meeting current survival and welfare needs. Sustainable irrigation management can help with climate change adaptation, labor, energy savings, and the production of higher-value and yield of crops to achieve zero hunger in water-scarce world. To ensure equal access to water and environmental sustainability, investments in expanded and enhanced irrigation must be matched by improvements in water governance. Sustainable irrigation must be able to cope with water scarcity, and be resilient to other resource scarcities throughout time in context of energy and finance. The themes and SDGs related to clean water, water resources sustainability, sustainable water usage, agricultural and rural development are all intertwined in the concept of “sustainable irrigation for higher yield.” Sustainable irrigation management refers to the capability of using water in optimum quantity and quality on a local, regional, national, and global scale to meet the needs of humans and agro-ecosystems at present and in the future to sustain life, protect humans and biodiversity from natural and human-caused disasters which threaten life to exist. Resultantly higher yields will ensure food security

Schemas Mediate the Link Between Procrastination and Depression: Results from the United States and Pakistan

The current study extended the Procrastination-Health model by examining a multiple mediation model, with two cognitive schemas (defectiveness; insufficient self-control) serving as mediators. The models were as follows: procrastination → defectiveness → depression; procrastination → insufficient self-control → depression. Participants included 412 (271 women, 141 men) United States (US) and 240 (107 women, 133 men) Pakistani college students, who responded via self-report questionnaires. In the US sample, results revealed a non-significant direct effect between procrastination and depression after consideration for the two cognitive schemas, suggesting the schemas completely mediated the model. Both defectiveness and insufficient self-control schemas were significant individual mediators. In the Pakistani model, results revealed a significant direct effect and indirect effect through the two cognitive schemas, indicating partial mediation. Only the indirect path through defectiveness schemas was significant in the Pakistani model. Given slight differences in the two models, a moderated-mediation model was analyzed to determine if the strength of the direct and indirect effects varied by nationality. The strength of the direct and indirect effects was not moderated by nationality. Overall, this is the first study to identify cognitive mediators in the procrastination-depression relationship. Such findings represent a significant extension of the Procrastination-Health model and offer some unique cognitive insights into culturally sensitive conceptualizations and treatments for depression

Schemas Mediate the Link between Procrastination and Depression: Results from the United States and Pakistan

The current study extended the Procrastination-Health model by examining a multiple mediation model, with two cognitive schemas (defectiveness; insufficient self-control) serving as mediators. The models were as follows: procrastination defectiveness depression; procrastination insufficient self-control depression. Participants included 412 (271 women, 141 men) United States (U.S.) and 240 (107 women, 133 men) Pakistani college students, who responded via self-report questionnaires. In the U.S. sample, results revealed a non-significant direct effect between procrastination and depression after consideration for the two cognitive schemas, suggesting the schemas completely mediated the model. Both defectiveness and insufficient self-control schemas were significant individual mediators. In the Pakistani model, results revealed a significant direct effect and indirect effect through the two cognitive schemas, indicating partial mediation. Only the indirect path through defectiveness schemas was significant in the Pakistani model. Given slight differences in the two models, a moderated-mediation model was analyzed to determine if the strength of the direct and indirect effects varied by nationality. The strength of the direct and indirect effects was not moderated by nationality. Overall, this is the first study to identify cognitive mediators in the procrastination-depression relationship. Such findings represent a significant extension of the Procrastination-Health model and offer some unique cognitive insights into culturally sensitive conceptualizations and treatments for depression

Bio-Oriented Synthesis of Novel (S)-Flurbiprofen Clubbed Hydrazone Schiff’s Bases for Diabetic Management: In Vitro and In Silico Studies

A new series of (S)-flurbiprofen derivatives 4a–4p and 5a–5n were synthesized with different aromatic or aliphatic aldehydes and ketones to produce Schiff’s bases and their structures were confirmed through HR-ESI-MS, 1H, and 13C-NMR spectroscopy. The α-glucosidase inhibitory activities of the newly synthesized compounds were scrutinized, in which six compounds 5k, 4h, 5h, 4d, 4b, and 5i showed potent inhibition in the range of 0.93 to 10.26 µM, respectively, whereas fifteen compounds 4c, 4g, 4i, 4j, 4l, 4m, 4o, 4p, 5c, 5d, 5j, 5l, 5m, 5n and 1 exhibited significant inhibitory activity with IC50 in range of = 11.42 to 48.39 µM. In addition, compounds 5g, 5f, 4k, 4n, and 4f displayed moderate-to-low activities. The modes of binding of all the active compounds were determined through the molecular docking approach, which revealed that two residues, specifically Glu277 and His351 are important in the stabilization of the active compounds in the active site of α-glucosidase. Furthermore, these compounds block the active site with high binding energies (−7.51 to −3.36 kcal/mol) thereby inhibiting the function of the enzyme

Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan experience

AIM: To assess the association between chronic hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC) in Pakistan, and the genotype distribution among these HCC patients

Novel hydrazone schiff’s base derivatives of polyhydroquinoline: synthesis, <i>in vitro</i> prolyl oligopeptidase inhibitory activity and their Molecular docking study

This research work reports the synthesis of new derivatives of the hydrazone Schiff bases (1–17) based on polyhydroquinoline nucleus through multistep reactions. HR-ESIMS,1H- and 13C-NMR spectroscopy were used to structurally infer all of the synthesized compounds and lastly evaluated for prolyl oligopeptidase inhibitory activity. All the prepared products displayed good to excellent inhibitory activity when compared with standard z-prolyl-prolinal. Three derivatives 3, 15 and 14 showed excellent inhibition with IC50 values 3.21 ± 0.15 to 5.67 ± 0.18 µM, while the remaining 12 compounds showed significant activity. Docking studies indicated a good correlation with the biochemical potency of compounds estimated in the in-vitro test and showed the potency of compounds 3, 15 and 14. The MD simulation results confirmed the stability of the most potent inhibitors 3, 15 and 14 at 250 ns using the parameters RMSD, RMSF, Rg and number of hydrogen bonds. The RMSD values indicate the stability of the protein backbone in complex with the inhibitors over the simulation time. The RMSF values of the binding site residues indicate that the potent inhibitors contributed to stabilizing these regions of the protein, through formed stable interactions with the protein. The Rg. analysis assesses the overall size and compactness of the complexes. The maintenance of stable hydrogen bonds suggests the existence of favorable binding interactions. SASA analysis suggests that they maintained stable conformations without large-scale exposure to the solvent. These results indicate that the ligand–protein interactions are stable and could be exploited to design new drugs for disease treatment. Communicated by Ramaswamy H. Sarma</p