Sustainable fermentation processing of two revalorized agro-industrial discards: carrot and brewer's yeast

An integrated and sustainable fermentation process was developed which enabled both the revalorization of two regional agro-industrial discards as well as by-product reuse. Carrot and brewer's yeast, which are commonly used for animal feed, were processed to obtain 77.5 L of ethanol, 450 kg of solid waste called bagasse, 970 L of liquid effluent called vinasse, and 39.8 kg CO2 per each ton of discarded carrot. Results showed that the obtained bagasse was suitable for feeding 55 animals (calfs). The dilution of vinasse with fresh water (1:5) satisfied the requirements necessary to be used as beverage for the same number of animals, leaving a remnant which could be newly diluted (1:5) and used to irrigate a 0.025-ha carrot crop, the land dimension required to grow 1 ton of carrot.Fil: Aimaretti, Nora Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Universidad del Centro Educativo Latinoamericano. Facultad de Química; ArgentinaFil: Clementz, Adriana Laura. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Universidad del Centro Educativo Latinoamericano. Facultad de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Codevilla, Agustin. Universidad del Centro Educativo Latinoamericano. Facultad de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rojas, Maria L.. Universidad Nacional de Educacion a Distancia; EspañaFil: Yori, Juan Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Simulación de la aplicación del tratamiento mecánico biológico a los residuos urbanos : El caso de la ciudad de General Pico

General Pico city has a stable population around 52,000 inhabitants. The final disposition of the domiciliary solid remaindes is an important problem to be solved in this city. The garbage is accumulated progressively having every time greater difficulty to be taken advantage of and recycled. The investigation presented here allowed to construct a computacional model that simulates the management and the present treatment of the urban remainders, including in he himself the application of Biological Mechanical Treatment (TMB) of different ways, offering the possibility in front of studying the behavior of this system different tie scenes with the population growth, the increase in the generation of remainders, the capacity of the present plant (“Cooperative of Recycled Work of Don Alberto Limitada”), and the incidence in the distribution of key factors like reduction, reusability, recycling and power recovery.Sociedad Argentina de Informática e Investigación Operativ

Simulación de la distribución de los diferentes Estéreo-Isómeros de Vitamina E y β-Caroteno en las lipoproteinas sanguíneas de vacas lecheras

La salud de los animales es un factor determinante en la calidad, seguridad e inocuidad de los alimentos de origen animal para consumo humano. Uno de los factores determinantes de la salud animal es la calidad de sus alimentos. La vitamina E, el β-caroteno y algunos minerales trazas como selenio (Se) y zinc (Zn), son esenciales para la salud y el desempeño productivo de las vacas lecheras. Los mismos forman parte de un sistema antioxidante celular, previniendo el accionar dañino de los radicales libres del oxígeno y favoreciendo el buen funcionamiento del sistema inmunitario. Entre los diferentes nutrientes con actividad antioxidante, el α-tocopherol y el β-caroteno juegan un rol preponderante en la prevención de la oxidación de los ácidos grasos poliinsaturados, presentes en las membranas celulares. La investigación aquí presentada permitió construir un modelo computacional que simula la distribución de los diferentes estéreo-isómeros sanguíneos de vitamina E en vacas lecheras durante el periparto, brindando la posibilidad de estudiar el comportamiento de este sistema frente a distintos escenarios vinculados con diferentes niveles de suplementación preparto.The wellbeing of the animals is a determining factor in the quality, security and health of foods animal origin for human consumption.. The vitamins A, E and some trace minerals like selenium (Se) and zinc (Zn), are essential for the health and the performance of the dairy cows. They belong to the cellular antioxidant system, preventing harmful driving of the free radicals of oxygen upon the good operation of the immune system. Among the different nutrients with antioxidant activity, α-tocopherol and the β- carotene plays a preponderant roll in the prevention of the oxidation of polyunsaturated fatty acids, present in cellular membranes. The investigation presented here allowed building up a computational model that simulates the distribution of the different sanguineous stereoisomer of vitamins during the peripartum in dairy cows, offering the possibility of studying the behavior of this system with different levels of supplementation prepartum.Sociedad Argentina de Informática e Investigación Operativ

Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7

The retromer complex mediates retrograde transport of transmembrane cargo from endosomes to the trans-Golgi network (TGN). Mammalian retromer is composed of a sorting nexin (SNX) dimer that binds to phosphatidylinositol 3-phosphate–enriched endosomal membranes and a vacuolar protein sorting (Vps) 26/29/35 trimer that participates in cargo recognition. The mammalian SNX dimer is necessary but not sufficient for recruitment of the Vps26/29/35 trimer to membranes. In this study, we demonstrate that the guanosine triphosphatase Rab7 contributes to this recruitment. The Vps26/29/35 trimer specifically binds to Rab7–guanosine triphosphate (GTP) and localizes to Rab7-containing endosomal domains. Interference with Rab7 function causes dissociation of the Vps26/29/35 trimer but not the SNX dimer from membranes. This blocks retrieval of mannose 6-phosphate receptors to the TGN and impairs cathepsin D sorting. Rab5-GTP does not bind to the Vps26/29/35 trimer, but perturbation of Rab5 function causes dissociation of both the SNX and Vps26/29/35 components from membranes through inhibition of a pathway involving phosphatidylinositol 3-kinase. These findings demonstrate that Rab5 and Rab7 act in concert to regulate retromer recruitment to endosomes

Factors associated with allergic rhinitis in colombian subpopulations aged 1 to 17 and 18 to 59

Background: Several studies have shown variations in the prevalence of allergic rhinitis (AR) around the world, and different potential predisposing factors. More studies are needed on risk factors, specifically in developing countries. This study explored the association of several factors and AR among urban residents in six cities of Colombia. Methodology: A cross-sectional study and a nested case-control study were carried out between 2009 and 2010 involving two Colombian subpopulations: children/adolescents and adults. Cases were affirmative respondents to “In the past 12 months, have you (or your child) had a problem with sneezing or a running or blocked nose, when you (or your child) did not have a cold or the flu?” “Controls” were subjects who never had been diagnosed with asthma, AR or atopic eczema by a physician, and whom did not report any symptoms in the past twelve months. Weighted logistic regression was used to assess the association of different factors with case/control status. Results: Factors associated with AR in children/adolescents were family history of AR, acetaminophen consumption and high socioeconomic status. Among adults, family history of asthma, AR and atopic eczema, and cetaminophen consumption were associated with AR. Consumption of cereals among children/adolescents and eating eggs among adults showed protective associations. Conclusions: Our findings suggest the presence of previously unknown cultural, environmental and family factors associated with the presence of AR in Colombia. © 2016, AMC. All rights reserved

Analgesia during Parturition in Domestic Animals: Perspectives and Controversies on Its Use

This article analyzes the physiological role of pain during parturition in domestic animals, discusses the controversies surrounding the use of opioids, non-steroidal anti-inflammatory drugs (NSAIDs), and local analgesics as treatments during labor, and presents the advantages and disadvantages for mother and offspring. Labor is a potentially stressful and painful event, due to the contractions that promote expulsion of the fetus. During labor, neurotransmitters such as the prostaglandins contribute to the sensitization of oxytocin receptors in the myometrium and the activation of nociceptive fibers, thus supporting the physiological role of pain. Endogenously, the body secretes opioid peptides that modulate harmful stimuli and, at the same time, can inhibit oxytocin’s action in the myometrium. Treating pain during the different stages of parturition is an option that can help prevent such consequences as tachycardia, changes in breathing patterns, and respiratory acidosis, all of which can harm the wellbeing of offspring. However, studies have found that some analgesics can promote myometrial contractility, increase expulsion time, affect fetal circulation, and alter mother–offspring recognition due to hypnotic effects. Other data, however, indicate that reducing the number of uterine contractions with analgesics increases their potency, thus improving maternal performance. Managing pain during labor requires understanding the tocolytic properties of analgesics and their advantages in preventing the consequences of pain.info:eu-repo/semantics/publishedVersio

An egg-shell bifunctional CeO2-modified NiPd/Al2O3 catalyst for petrochemical processes involving selective hydrogenation and hydroisomerization

The catalytic performance during the 1-butyne hydrogenation using two reduced Al2O3-supported Pd-based catalysts was carried out in a total recirculation system with an external fixed-bed reactor. The lab-prepared egg-shell NiPd/CeO2-Al2O3 catalyst (NiPdCe) with Pd loading = 0.5 wt%, Ni/Pd atomic ratio = 1 and CeO2 loading = 3 wt% was synthesized and characterized, and it was compared with an egg-shell Al2O3-supported Pd based commercial catalyst (PdCC). The reduced catalysts were characterized by X-ray diffraction, X-ray photoelectron spectroscopy, and high-resolution transmission electron microscopy. The textural characteristics and ammonia temperature-programmed desorption profiles of the fresh (unreduced) catalysts were also obtained. Both catalysts show high 1-butyne conversion and selectivity to 1-butene, but the catalysts also present important differences between hydroisomerizing and hydrogenating capabilities. NiPdCe catalyst shows higher capability for hydroisomerization reactions, while the PdCC catalyst exhibits higher hydrogenating capability. The observed catalytic performances can be interesting for some industrial processes and can provide a guideline for the development of a Pd-based catalyst with specific catalytic properties.Fil: Méndez, Franklin J.. Universidad Nacional Autónoma de México; México. Instituto Venezolano de Investigaciones Científicas; VenezuelaFil: Alves, Javier Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaFil: Rojas Challa, Yahse Vitah Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Corona, Oscar. Universidad Nacional Autónoma de México; México. Instituto Tecnológico Venezolano Del Petróleo; VenezuelaFil: Villasana, Yanet. Instituto Venezolano de Investigaciones Científicas; Venezuela. Universidad Regional Amazonica Ikiam; EcuadorFil: Guerra, Julia. Universidad Simón Bolívar; VenezuelaFil: Garcia Colli, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaFil: Martinez, Osvaldo Miguel. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Brito, Joaquín L.. Instituto Venezolano de Investigaciones Científicas; Venezuel

Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL

Microbial pathogens employ sophisticated virulence strategies to cause infections in humans. The intracellular pathogen Legionella pneumophila encodes RidL to hijack the host scaffold protein VPS29, a component of retromer and retriever complexes critical for endosomal cargo recycling. Here, we determined the crystal structure of L. pneumophila RidL in complex with the human VPS29?VPS35 retromer subcomplex. A hairpin loop protruding from RidL inserts into a conserved pocket on VPS29 that is also used by cellular ligands, such as Tre-2/Bub2/Cdc16 domain family member 5 (TBC1D5) and VPS9-ankyrin repeat protein for VPS29 binding. Consistent with the idea of molecular mimicry in protein interactions, RidL outcompeted TBC1D5 for binding to VPS29. Furthermore, the interaction of RidL with retromer did not interfere with retromer dimerization but was essential for association of RidL with retromer-coated vacuolar and tubular endosomes. Our work thus provides structural and mechanistic evidence into how RidL is targeted to endosomal membranes.ACKNOWLEDGMENTS: We thank Ander Vidaurrazaga (Centro de Investigación Cooperativa en Biociencias) for technical assistance and Devanand Bondage (National Institute of Child Health and Human Development) for proliferation assays of Legionella pneumophila. This study made use of the Diamond Light Source (Oxfordshire, United Kingdom), the European Synchrotron Radiation Facility (Grenoble, France), and the ALBA synchrotron beamline BL13-XALOC, funded in part by the Horizon 2020 programme of the European Union, iNEXT (H2020 Grant 653706). We thank all the staff from these facilities for technical and human support. This work was supported by the Spanish Ministry of Economy and Competitiveness Grant BFU2014-59759-R (to A.H.); the Severo Ochoa Excellence Accreditation SEV-2016-0644; and the Intramural Program of the Eunice Kennedy Shriver National Institute of Child Health and Human development (Projects ZIA HD001607 and ZIA HD008893). M.R.-M. is supported by a pre-doctoral fellowship from the Basque Government (PRE_2016_2_0249)

Knowledge, Attitudes, and Practices Regarding COVID-19 Among Healthcare Workers in Venezuela:An Online Cross-Sectional Survey

Background: The deterioration of Venezuela's health system in recent years undoubtedly contributes to an increased impact of the COVID-19 pandemic. Understanding healthcare workers' (HCWs) knowledge, attitudes, and practices (KAPs) toward COVID-19 in the early stages of the pandemic could inform their medical training and improve their preparedness. Methods: A online national cross-sectional survey was conducted between May 26th and May 30th, 2020, to assess KAPs among HCWs in Venezuela. Results: A total of 1,441 HCWs from all 24 regions of the country responded to the survey. The mean age of the HCWs was 44 (SD [standard deviation] 14) years; most were women (66.4%). Most HCWs were specialized doctors (48%), followed by nurses (13%) and resident doctors (12.3%). The majority of HCWs had good knowledge (76.3%), obtained information mainly from scientific literature (85.4%); had negative attitudes (53.6%), felt uncomfortable with their work during the current pandemic (59.8%); and reported appropriate practices (76.9%). However, participation in COVID-19 related training was absent in more than half of the HCWs. Positive attitudes were significantly more frequent in frontline workers than in non-frontline workers (p = 0.001). Bioanalysts, students, and doctors were more likely to have good knowledge; participating in training was a predictor for positive attitudes and older age was an appropriate practice predictor. Conclusions: HCWs, knowledge in Venezuela could be improved by strengthening education and training programs. Strategies should focus on reducing fear and improving attitudes toward the care of COVID-19 patients, as well as the promotion of preventive practices

Methylation of subtelomeric chromatin modifies the expression of the lncRNA TERRA, disturbing telomere homeostasis

The long noncoding RNA (lncRNA) telomeric repeat-containing RNA (TERRA) has been associated with telomeric homeostasis, telomerase recruitment, and the process of chromosome healing; nevertheless, the impact of this association has not been investigated during the carcinogenic process. Determining whether changes in TERRA expression are a cause or a consequence of cell transformation is a complex task because studies are usually carried out using either cancerous cells or tumor samples. To determine the role of this lncRNA in cellular aging and chromosome healing, we evaluated telomeric integrity and TERRA expression during the establishment of a clone of untransformed myeloid cells. We found that reduced expression of TERRA disturbed the telomeric homeostasis of certain loci, but the expression of the lncRNA was affected only when the methylation of subtelomeric bivalent chromatin domains was compromised. We conclude that the disruption in TERRA homeostasis is a consequence of cellular transformation and that changes in its expression profile can lead to telomeric and genomic instability