Haematobia irritans clone Hi-18S-ab-fr 18S ribosomal RNA gene, partial sequence

GenBank: EU013947.1 Haematobia irritans clone Hi-18S-ab-fr 18S ribosomal RNA gene, partial sequenceFil: Sonvico, A.. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Filiberti, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentin

Micromagnetism in (001) magnetite by spin-polarized low-energy electron microscopy

Spin-polarized low-energy electron microscopy was used to image a magnetite crystal with (100) surface orientation. Sets of spin-dependent images of magnetic domain patterns observed in this surface were used to map the direction of the magnetization vector with high spatial and angular resolution. We find that domains are magnetized along the surface [110] directions, and domain wall structures include 90{\deg} and 180{\deg} walls. A type of unusually curved domain walls are interpreted as N\'eel-capped surface terminations of 180{\deg} Bloch walls.Comment: 17 pages, 4 figures. Presented at the LEEM-PEEM 8 conferenc

Hydrogen-induced reversible spin-reorientation transition and magnetic stripe domain phase in bilayer Co on Ru(0001)

Imaging the change in the magnetization vector in real time by spin-polarized low-energy electron microscopy, we observed a hydrogen-induced, reversible spin-reorientation transition in a cobalt bilayer on Ru(0001). Initially, hydrogen sorption reduces the size of out-of-plane magnetic domains and leads to the formation of a magnetic stripe domain pattern, which can be understood as a consequence of reducing the out-of-plane magnetic anisotropy. Further hydrogen sorption induces a transition to an in-plane easy-axis. Desorbing the hydrogen by heating the film to 400 K recovers the original out-of-plane magnetization. By means of ab-initio calculations we determine that the origin of the transition is the local effect of the hybridization of the hydrogen orbital and the orbitals of the Co atoms bonded to the absorbed hydrogen.Comment: 5 figure

D. melanogaster and H. irritans are more sensitive to Phloxine B phototoxicity than C. capitata

The use of photosensitizers is emerging as an ambient friendly possible new tool to control several insect species. Xanthene derivatives have been the most extensively studied (Heitz, 1997; Ben Amor and Jori, 2000, for review). The toxicity of these dyes has been tested in the adult stage of different insect orders (ants: David and Heitz, 19 78; boll weevil: Callaham et al., 1975; cockroaches: Ballard et al. , 1988; grasshoppers: Capinera and Squitier, 2000). In particular, xanthenes showed acute photo-toxicity agai nst several Dipterans ( Musca domestica : Pimprikar et al. , 1980; Culex and Aedes : Pimprikar et al. , 1979; Ceratitis capitata : Liquido et al. , 1995; Anastrepha ludens : Mangan and Moreno, 1995). Studies of the effects in the dark as well as in immature stages must be carried out in order to understand which phototoxic pathway is triggered and which main cellular and sub- cellular targets are affecte d. However, the effects of these dyes against immature stages of dipterans have been seldom analyzed. Among the few studies, Pimprikar et al. (1980) studied Erythrosin B effects in Musca domestica , whereas Berni et al. (2003) studied the toxic ity of Phloxine B in Ceratitis capitata.Fil: Pujol Lereis, Luciana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Rabossi, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Filiberti, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Argaraña, Carlos Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentin

Los mapas mentales como estrategia didáctica para el mejoramiento de la comprensión lectora en textos narrativos

La lectura representa para los estudiantes una herramienta fundamental en el proceso académico, es gracias a ella que se logra conocer y evaluar los pensamientos y filosofías de diferentes autores a través del tiempo, asimismo la lectura es fuente indispensable para la investigación y la sociedad. Sin embargo, este proceso presenta dificultades para algunos estudiantes, la mala comprensión y la falta de interpretación se encuentran presentes en dicho proceso y es debido a estos problemas que se pretende con este proyecto mejorar la comprensión de lectura y reducir los problemas ya mencionados.Reading presents to students a fundamental tool in the academic process, it is thanks to her that she gets to know and evaluate the thoughts and philosophies of different authors over time, also reading is indispensable source for research and society. However, this process presents difficulties for some students, misunderstanding and lack of interpretation are present in this process and it is because of these problems that this project aims to improve reading comprehension and reduce the aforementioned problems.Reading presents to students a fundamental tool in the academic process, it is thanks to her that she gets to know and evaluate the thoughts and philosophies of different authors over time, also reading is indispensable source for research and society. However, this process presents difficulties for some students, misunderstanding and lack of interpretation are present in this process and it is because of these problems that this project aims to improve reading comprehension and reduce the aforementioned problems

Metamorphosis and gonad maturation in the horn fly Haematobia irritans

The bloodsucking horn fly, Haematobia irritans (L.) (Diptera: Muscidae), is one of the most damaging pests of pasture cattle in many areas of the world. Both male and female imagoes spend their adult stage on the host, while immature stages develop in dung. Our goal was to determine if the progress of H. irritans gonad maturation can be correlated with eye and cuticle pigmentation events that occur during development of the imago within the puparium. The progression of germline cell divisions in immature gonads was analyzed from the beginning of the third larval instar (48 hours after egg hatch) until imago ecdysis. In the developing male larval gonad, meiosis began 72 hours after egg hatch, whereas in females oogonia were premeiotic at 72 hours. Meiosis was not detected in females until the mid-pharate adult stage, 120 hours after puparium formation. Therefore, gonad maturation in females appears to be delayed 144 hours with respect to that in males. In the stages within the puparium, the timing of germline cell division events was correlated with the progress of pigmentation of the eyes and cuticle as external markers.Fil: Basso Abraham, Alicia Leonor Rufina. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Forneris, Natalia Soledad. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Filiberti, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina; ArgentinaFil: Argaraña, Carlos Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina; ArgentinaFil: Rabossi, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentin

Tuning the N\'eel temperature in an antiferromagnet: the case of NixCo1-xO microstructures

We show that it is possible to tune the N\'eel temperature of nickel(II)-cobalt(II) oxide films by changing the Ni to Co ratio. We grow single crystalline micrometric triangular islands with tens of nanometers thickness on a Ru(0001) substrate using high temperature oxygen-assisted molecular beam epitaxy. Composition is controlled by adjusting the deposition rates of Co and Ni. The morphology, shape, crystal structure and composition are determined by low-energy electron microscopy and diffraction, and synchrotron-based x-ray absorption spectromicroscopy. The antiferromagnetic order is observed by x-ray magnetic linear dichroism. Antiferromagnetic domains up to micrometer width are observed

Towards a common European ethical and legal framework for conducting clinical research : the GATEKEEPER experience

This paper examines the ethical and legal challenges encountered during the GATEKEEPER Project and how these challenges informed the development of a comprehensive framework for future Large-Scale Pilot (LSP) projects. GATEKEEPER is a LSP Project with 48 partners conducting 30 implementation studies across Europe with 50,000 target participants grouped into 9 Reference Use Cases. The project underscored the complexity of obtaining ethical approval across various jurisdictions with divergent regulations and procedures. Through a detailed analysis of the issues faced and the strategies employed to navigate these challenges, this study proposes an ethical and legal framework. This framework, derived from a comparative analysis of ethical application forms and regulations, aims to streamline the ethical approval process for future LSP research projects. By addressing the hurdles encountered in GATEKEEPER, the proposed framework offers a roadmap for more efficient and effective project management, ensuring smoother implementation of similar projects in the future

Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9 is an effector protein that targets invading DNA and plays a major role in the prokaryotic adaptive immune system. Although Streptococcus pyogenes CRISPR–Cas9 has been widely studied and repurposed for applications including genome editing, its origin and evolution are poorly understood. Here, we investigate the evolution of Cas9 from resurrected ancient nucleases (anCas) in extinct firmicutes species that last lived 2.6 billion years before the present. We demonstrate that these ancient forms were much more flexible in their guide RNA and protospacer-adjacent motif requirements compared with modern-day Cas9 enzymes. Furthermore, anCas portrays a gradual palaeoenzymatic adaptation from nickase to double-strand break activity, exhibits high levels of activity with both single-stranded DNA and single-stranded RNA targets and is capable of editing activity in human cells. Prediction and characterization of anCas with a resurrected protein approach uncovers an evolutionary trajectory leading to functionally flexible ancient enzymes.This work has been supported by grant nos. PID2019-109087RB-I00 (to R.P.-J.) and RTI2018-101223-B-I00 and PID2021-127644OB-I00 (to L.M.) from the Spanish Ministry of Science and Innovation. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 964764 (to R.P.-J.). The content presented in this document represents the views of the authors, and the European Commission has no liability in respect to the content. We acknowledge financial support from the Spanish Foundation for the Promotion of Research of Amyotrophic Lateral Sclerosis. A.F. acknowledges Spanish Center for Biomedical Network Research on Rare Diseases (CIBERE) intramural funds (no. ER19P5AC756/2021). F.J.M.M. acknowledges research support by Conselleria d’Educació, Investigació, Cultura i Esport from Generalitat Valenciana, research project nos. PROMETEO/2017/129 and PROMETEO/2021/057. M.M. acknowledges funding from CIBERER (grant no. ER19P5AC728/2021). The work has received funding from the Regional Government of Madrid (grant no. B2017/BMD3721 to M.A.M.-P.) and from Instituto de Salud Carlos III, cofounded with the European Regional Development Fund ‘A way to make Europe’ within the National Plans for Scientific and Technical Research and Innovation 2017–2020 and 2021–2024 (nos. PI17/1659, PI20/0429 and IMP/00009; to M.A.M.-P. B.P.K. was supported by an MGH ECOR Howard M. Goodman Award and NIH P01 HL142494

The state of the Martian climate

60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes