Phytochemical screening and properties of botanical crude extracts and ethyl acetate fractions isolated by deep eutectic solvent

Botanicals, being safe because of their natural origin, are associated with potential health benefits due to their isoflavones-rich content. Isoflavones as plant-generated secondary metabolites are formed to defend against environmental stress such as UV radiation, mechanical damage, or any other attack from the surroundings. Kudzu (Pueraria roots and flowers), red clover (Trifolium pratense), and soy (Glycine max) are of significant importance in the medical field. Isoflavones were reported to exhibit a variety of biological functions, e.g., antiaging, anticancer, and microbiome modulations. This study focuses attention on the extraction and quantification of isoflavones from kudzu roots (KR), kudzu flowers (KF), red clover (RC), and soy molasses (SM), and determining their total polyphenols (TPC), flavonoids (TFC), and antioxidant activity (AOA). Novel green ultrasound-assisted extraction (UAE) technology was developed to extract and quantify the isoflavones. The results showed the quantity of isoflavones in KR, KF, RC, and SM crude extracts to be 1.01%, 2.9%, 0.70% and 0.67%, respectively. The fractionation using ethyl acetate (EA) improved the yield that became 1.48%, 2.5%, 1.3% and 0.89% in the KR, RC and SM extract fractions, respectively. This investigation eventually showed that our green UAE methodology is a safe and efficient method for recovering isoflavones. The total isoflavone contents were recorded as follows: KFKRRCSM. We concluded that these botanicals are a crucial source of isoflavones that exhibit strong antioxidant properties

Lecture 5. Natural Deep Eutectic Solvents in Extraction of Biologically Active Compounds

The valorization of agricultural and fishery by-products by extracting their bioactive compounds is a very interesting alternative to their incineration or even composting. Bioactive compounds have been studied intensively due to their biological properties, capable of providing multiple health benefits. They can be extracted from by-products of agricultural, food, and fishing industries, such as crustaceans’ shells, plants, algae, or microalgae by-products, which can correctly contribute to a circular economy based on zero waste. For applications, mainly in the food, cosmetic and pharmaceutical industries, these compounds are generally extracted using organic solvents or also known as conventional solvents (methanol, acetone, benzene, chloroform, petroleum ether, and hexane), which many times are rejected for being flammable, explosive, poorly biodegradable and for the toxicity they can produce to the final consumer. Green technologies such as supercritical fluid extraction, ultrasonic extraction and microwave field extraction can increase process selectivity, increase productivity and increase efficiency, but they are costly and energy intensive. Alternative solvents, sometimes referred to as "green solvents", such as ionic liquid (IL) solvents, deep eutectic solvents (DES), and natural eutectic solvents (NADES) can be used as a solution to this problem [1]. In this review, developments related to the use of deep eutectic solvents for the extraction of different bioactive compounds and our own studies on ultrasound-assisted extraction of isoflavones of kudzu roots and flowers, red clover flowers and soy molasses as well as piperine from black pepper are briefly discussed

USE OF RED CLOVER ISOFLAVONES (TRIFOLIUM PRATENSE) FOR THE CORRECTION OF BIOCHEMICAL PARAMETERS IN RATS WITH EXPERIMENTAL TYPE 2 DIABETES

Type 2 diabetes (T2D) and its complications are one of the leading causes of death and disability worldwide. The pathogenesis of T2D is based on hyperglycemia-induced chronic oxidative stress and inflammation [1]. Due to the need to develop new pathogenesis-oriented drugs for the treatment and prevention of T2D, the attention of researchers is attracted by isoflavones (IF) – bioactive substances of plant origin with antioxidant and anti-inflammatory activity [2].The research was supported by the grant of the Russian Science Foundation № 24-25-20147 (https://rscf.ru/project/24-25-20147) and the Government of the Sverdlovsk region

PREPARATION OF SYNBIOTIC FOOD PRODUCTS FROM NON-DAIRY SOURCE, CURRENT SCENARIO, CHALLENGES AND FUTURE PERSPECTIVE

Probiotics, beneficial bacteria is vital for gastrointestinal health, that decompose macromolecule to food are commonly sourced from non-pathogenic microorganisms like lactic acid bacteria (LAB). LAB, renowned for their fermentative capabilities, yields various bioactive compounds, enhancing food quality and promoting health1. Despite their traditional association with dairy products, concerns over lactose intolerance, follow to vegan diet and antibiotic resistance drive the demand for non-dairy probiotics food and beverages2.This research was funded by the Ministry of Science and Higher Education of the Russian Federation (Prioгity-2030 Program)

COMPARATIVE ANALYSIS AND OPTIMIZATION OF GREEN EXTRACTION TECHNOLOGIES FOR ISOFLAVONES FROM PLANT RAW MATERIALS

Isoflavones, a significant class of plant polyphenols, are recognized for their health benefits, including cholesterol reduction, cancer prevention, and mitigation of osteoporosis and menopause symptoms. This study focuses on optimizing the supercritical fluid extraction (SFE) of isoflavones from kudzu (Pueraria montana) and red clover (Trifolium pratense) flowers, which are rich in these compounds. The extraction process was optimized by varying pressure, time, and co-solvent types. Comparisons were made with ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES) and conventional extraction methods to evaluate yield, antioxidant activities, total phenolic content, and total flavonoid content in the extracts.The research was supported by the grant of the Russian Science Foundation № 24-25-20147 (https://rscf.ru/project/24-25-20147) and the Government of the Sverdlovsk region

ISOLATION, CHARACTERIZATION, AND ASSESSMENT OF CYTOTOXICITY OF ISOFLAVONES DERIVED FROM KUDZU ROOT AND SOY MOLASSES

We acknowledge the financial support of the Russian Science Foundation, Grant No.20-66-47017

Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology

Bioactive compounds like piperine (alkaloids) offer a variety of health benefits due to their biological and pharmacological potential. Piperine has been revealed to have anti-inflammatory, anti-aging, anti-diabetes, anti-bacterial, anti-ulcer, and anti-carcinogenic characteristics. Recent research has been conducted to extract piperine using effective and environmentally friendly techniques. In this study, we sought to assess the potential and efficacy of natural deep eutectic solvents to extract piperine from black pepper seeds using an ultrasound-assisted extraction technique. A Box–Behnken design combined with response surface methodology was used to evaluate the optimum extraction conditions of piperine. Extraction efficiency was evaluated based on the extraction yields of piperine, antioxidant activity, total polyphenols, and total flavonoids. The results showed that the choline chloride-citric acid-1,2-propylene glycol combination (1:2:2 molar ratio) with 25% (v/v) of water was the most effective at extracting piperine from black pepper. It was found that the extraction yield of piperine was significantly influenced by the liquid–solid ratio and extraction time. The optimal extraction conditions were determined and it was found that antioxidant activities and total polyphenol content in the piperine-rich extracts were remarkably related to the piperine content. The piperine extract purity was found to be 90%. Our results indicate that black pepper could be used as a functional food application