Morphological and phylogenetic evidence for two new <I>Lactarius</I> species (Russulales, Basidiomycota) from India

Lactarius ferruginascens sp. nov. and L. indoaquosus sp. nov. are described from the East and South districts of Sikkim, a small Himalayan state in India. Macro- and micromorphological descriptions coupled with the illustrations and nrITS-based molecular analyses are given for both species. Lactarius ferruginascens is a species in L. subg. Plinthogalus with striking features such as a sticky pileus surface caused by the ixohymeniderm structure of the pileipellis, watery white latex which is turning rusty brown on the lamellae and the occurrence under Abies sp. in the subalpine mixed forest. Lactarius indoaquosus is a new representative of L. subg. Russularia with completely transparent and unchanging latex and is furthermore characterized by basidiospores with a zebroid ornamentation, the absence of hymenial macrocystidia and the occurrence under Castanopsis sp. in the temperate broadleaf forest. Both species are illustrated and compared with related taxa.</p

Rhodotorula sampaioana f.a., sp. nov., a novel red yeast of the order Sporidiobolales isolated from Argentina and India

A set of four strains representing a novel basidiomycetous yeast species Rhodotorula sampaioana f. a., sp. nov. were isolated from two different habitats, subsurface waters of Lake Negra in Argentina, and the gut of a xylophagous termite in India. Phylogenetic analyses of LSU and ITS sequences showed that they belonged to the genus Rhodotorula of the order Sporidiobolales (subphylum Pucciniomycotina) and the closest known relative being R. kratochvilovae. The new species differed from R. kratochvilovae CBS 7436 (AF071436, AF444520) by nine nucleotide substitutions and one deletion (1.7 % sequence variation) in a 593 bp D1/D2 region, and by five nucleotide substitutions and three deletions (1.3 %) in a 592 bp ITS region, respectively. Several morphological and physiological differences were also observed between R. kratochvilovae and the strains obtained during this study. These data support the proposal of Rhodotorula sampaioana as a novel species, with CRUB 1124 as the holotype, CBS 10798 as ex-type, and NFCCI 4872 as an additional strain. The GenBank accession numbers of the LSU and ITS sequences of Rhodotorula sampaioana f. a., sp. nov. are EF595748 and MW879331. The MycoBank number is MB 838533.Fil: Tiwari, Snigdha. Savitribai Phule Pune University; IndiaFil: Baghela, Abhishek. Savitribai Phule Pune University; IndiaFil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentin

A Rare Stinkhorn Fungus Itajahya rosea Attract Drosophila by Producing Chemical Attractants

Itajahya rosea was found growing in association with Leucaena leucocephala plants at Savitribai Phule Pune University campus in India. The species identity was confirmed by phylogenetic analysis based on ITS and LSU regions of rDNA, wherein, our fugus was placed along with I. rosea in phylogenetic tree. It represents first record of I. rosea from India. Frequent visitation by Drosophila species on I. rosea fruiting body particularly on gleba was observed. The Drosophila got attracted to the detached gleba under the laboratory conditions and even sometimes, they prefer to sit over the gleba as compare to their food banana. It suggested that I. rosea gleba or pseudostipe produces some compounds for attraction and feeding behavior of Drosophila species. Therefore, we characterized the volatile attractants produced by gleba and pseudostipe of I. rosea by GC-MS analysis. Nineteen compounds were identified from gleba while nine compounds were recovered from the pseudostipe. Out of them, blends of three abundant odor producing volatile compounds were reported namely, Hexadecane, Pentadecane and Nonadecane, which are responsible for attraction of Drosophila toward the gleba. Three fatty acids namely 9,12-octadecadienoic acid (Z,Z), hexadecanoic acid and benzoic acid ethyl ester produced are served as an appetitive signal through olfactory response of Drosophila, so the flies were feed on the gleba. Two pheromones’ compounds, heneicosane and (+)-(5S,9S)-5,9-dimethylpentadecane, were also reported in pseudostipe and gleba, respectively, which play a role in Drosophila for breeding. Our study highlights an intriguing chemical ecology of fungus–Drosophila interaction

Morphological and phylogenetic evidence for two new Lactarius species (Russulales, Basidiomycota) from India

Lactarius ferruginascens sp. nov. and L. indoaquosus sp. nov. are described from the East and South districts of Sikkim, a small Himalayan state in India. Macro- and micromorphological descriptions coupled with the illustrations and nrITS-based molecular analyses are given for both species. Lactarius ferruginascens is a species in L. subg. Plinthogalus with striking features such as a sticky pileus surface caused by the ixohymeniderm structure of the pileipellis, watery white latex which is turning rusty brown on the lamellae and the occurrence under Abies sp. in the subalpine mixed forest. Lactarius indoaquosus is a new representative of L. subg. Russularia with completely transparent and unchanging latex and is furthermore characterized by basidiospores with a zebroid ornamentation, the absence of hymenial macrocystidia and the occurrence under Castanopsis sp. in the temperate broadleaf forest. Both species are illustrated and compared with related taxa

Morphological and phylogenetic studies of Lycoperdon rupicola: first report for the Indian mycobiota

Lycoperdon rupicola Jeppson, E. Larss. & M.P. Martín growing on mossy bed attached to rocks or soil, is reported here for the first time from India with its morphological details, supporting illustrations and phylogenetic evidences. Similar looking or allied taxa are compared. The ITS- and LSU-rDNA based phylogenetic analysis of our collection also confirms its conspecificity with its European counterpart

Cavitary Pulmonary Zygomycosis Caused by Rhizopus homothallicus▿

We report the first two proven cases of cavitary pulmonary zygomycosis caused by Rhizopus homothallicus. The diagnosis in each case was based on histology, culture of the causal agent, and the nucleotide sequence of the D1/D2 region of the 28S ribosomal DNA