Chemosensetizing and cardioprotective effects of resveratrol in doxorubicin- treated animals

BACKGROUND: Doxorubicin (DOX), an anthracycline antibiotic is one of the most effective anticancer drug used in the treatment of variety of cancers .Its use is limited by its cardiotoxicity. The present study was designed to assess the role of a natural product resveratrol (RSVL) on sensitization of mammary carcinoma (Ehrlich ascites carcinoma) to the action of DOX and at the same time its protective effect against DOX-induced cardiotoxicity in rats. METHODS: Ehrlich ascites carcinoma bearing mice were used in this study. Percent survival of tumor bearing mice was used for determination of the Cytotoxic activity of DOX in presence and absence of RSVL. Uptake and cell cycle effect of DOX in tumor cells in the presence of RSVL was also determined. Histopatholgical examination of heart tissues after DOX and/or RSVL therapy was also investigated. RESULTS: DOX at a dose level of 15 mg/kg increased the mean survival time of tumor bearing mice to 21 days compared with 15 days for non tumor-bearing control mice. Administration of RSVL at a dose level of 10 mg/kg simultaneously with DOX increased the mean survival time to 30 days with 70% survival of the tumor-bearing animals. RSVL increased the intracellular level of DOX and there was a strong correlation between the high cellular level of DOX and its cytotoxic activity. Moreover, RSVL treatment showed 4.8 fold inhibition in proliferation index of cells treated with DOX. Histopathological analysis of rat heart tissue after a single dose of DOX (20 mg/kg) showed myocytolysis with congestion of blood vessels, cytoplasmic vacuolization and fragmentation. Concomitant treatment with RSVL, fragmentation of the muscle fiber revealed normal muscle fiber. CONCLUSION: This study suggests that RSVL could increase the cytotoxic activity of DOX and at the same time protect against its cardiotoxicity

Acylated pregnane glycosides from Caralluma quadrangula

In a previous study, the methanolic extract as well as the chloroform fraction of the aerial parts of Caralluma quadrangula (Forssk.) N.E.Br. indigenous to Saudi Arabia showed significant in vitro cytotoxic activity against breast cancer (MCF7) cell line. In a biologically-guided fractionation approach, four acylated pregnane glycosides were isolated from the chloroform fraction of C. quadrangula. The structures of the isolated compounds were elucidated by the analysis of their MS and NMR data. The compounds were identified as 12,20-di-O-benzoylboucerin 3-O-?-D-digitoxopyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (1), 12,20-di-O-benzoylboucerin 3-O-?-D-cymaropyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (2), 12,20-di-O-benzoylboucerin 3-O-?-D-glucopyranosyl-(1?4)-?-D-digitoxopyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (3) and 12,20-di-O-benzoyl-3?,5?,12?,14?,20-pentahydroxy-(20R)-pregn-6-ene 3-O-?-D-glucopyranosyl-(1?4)-?-D-digitoxopyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (4). The isolated compounds were tested for their cytotoxic activity against breast cancer (MCF7) cell line

Acylated pregnane glycosides from Caralluma quadrangula

In a previous study, the methanolic extract as well as the chloroform fraction of the aerial parts of Caralluma quadrangula (Forssk.) N.E.Br. indigenous to Saudi Arabia showed significant in vitro cytotoxic activity against breast cancer (MCF7) cell line. In a biologically-guided fractionation approach, four acylated pregnane glycosides were isolated from the chloroform fraction of C. quadrangula. The structures of the isolated compounds were elucidated by the analysis of their MS and NMR data. The compounds were identified as 12,20-di-O-benzoylboucerin 3-O-?-D-digitoxopyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (1), 12,20-di-O-benzoylboucerin 3-O-?-D-cymaropyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (2), 12,20-di-O-benzoylboucerin 3-O-?-D-glucopyranosyl-(1?4)-?-D-digitoxopyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (3) and 12,20-di-O-benzoyl-3?,5?,12?,14?,20-pentahydroxy-(20R)-pregn-6-ene 3-O-?-D-glucopyranosyl-(1?4)-?-D-digitoxopyranosyl-(1?4)-?-D-canaropyranosyl-(1?4)-?-D-cymaropyranoside (4). The isolated compounds were tested for their cytotoxic activity against breast cancer (MCF7) cell line

International Journal of Multidisciplinary and Current Research Effect of solar drying using a natural convective solar drier on bacterial load and chemical composition of bayad (Bagrus bayad) fish flakes

Abstract Solar drying experiments of bayad (Bagrus bayad) fish flakes using a natural convective solar drier were conducted a