Evaluation of antimicrobial activity of a lichen used as a spice (Platismatia glauca)

Background: Lichen is a complex symbiotic relationship arose from algae or cyanobacteria that live together with some fungal species. Some of them are edible and consumed as spice such asPlatismatia glauca. The current study aimed to evaluate it’s the antimicrobial properties of the methanolic extract of lichen thalli of P. glauca against some referenced bacterial and yeast strains.Methods: Disc diffusion test, minimum inhibitory (MIC) and minimum bactericidal (MBC) or minimum fungicidal (MFC) tests were carried out to evaluate the antimicrobial activity of lichen.Results: All tested microorganisms exhibited varying degrees of susceptibility. Among the tested strains, the most susceptible bacterium -using the disc diffusion assay- was Staphylococcus saprophyticus (18.5±1.0 mm), followed by Staphylococcus aureus (14.5±0.5 mm), Shigella flexneri(12.5±1.5 mm), Streptococcus pneumoniae (12.0±1.0 mm), Proteus vulgaris (11.5±0.5 mm),Salmonella Typhimurium (11.5±0.5 mm), Bacillus cereus (11.0±1.0 mm) and Escherichia coli(11.0±0.0 mm), respectively. It also showed high antifungal activity against Candida albicans(22.5±0.5 mm).  The MIC, MBC and MFC were promising, which were as low as 3.125 to 12.5 mg/ml for MIC and 6.25 to 12.5 mg/ml for MBC and MFC.Conclusion: From the obtained results, it is concluded that the lichen thalli of Platismatia glaucapossesses a remarkable antimicrobial activity and it may be considered as a source of potential antimicrobial agents.Keywords: Antibacterial, Antifungal, Lichen, Platismatia glauc

Evaluation of antimicrobial and antioxidant properties of leaves of Emex spinosa and fruits of Citrillus colocynthis from Saudi Arabia

The crude methanol extract of Citrullus colocynthis fruit and Emex spinosa leaves were examined for antimicrobial and antioxidant potentialities. The phytochemical analysis revealed presence of some bioactive principles, such as alkaloids, flavonoids and anthraquinones for E. spinosa and saponin, flavonoids, terpenoids and alkaloids for C. colocynthis. The antimicrobial activities were determined against seven bacterial strains (Proteus vulgaris NCTC 8196, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumonia ATCC 53651, Salmonella typhi NCTC 0650, Staphylococcus aureus ATCC 25923 and Bacillus cereus NCTC 8236) and one fungal strain (Candida albicans ATCC 7596). E. spinosa leaf methanol extract was most active against fungus, whileC. colocynthis fruit methanol extract was most active against bacteria, particularly E. coli ATCC 25922 and P. aeruginosa ATCC 27853. The antioxidant properties of extracts were investigated in vitro using1,1-diphenyl, 2-picryl hydrazyl (DPPH) radical scavenging assay and in vivo in rats using serological and enzymatic tests. Both plant extracts showed considerable antioxidant activities. The promising findings of this investigation could be used as a novel natural antimicrobial and antioxidant agents.Keywords: Emex spinosa, Citrillus colocynthis, antimicrobial, antioxidant activity.African Journal of Biotechnology Vol. 12(34), pp. 5308-531

Antimicrobial, antioxidant and phytochemical investigation of Balanites aegyptiaca (L.) Del. edible fruit from Sudan

The methanol extract of the fruit (edible mesocarp) of Balanites aegyptiaca (L.) Del., a popular plant from Sudan, widely employed in Sudanese folk medicine was screened for antimicrobial, antioxidant and some active phytochemical compounds. The extract showed the presence of saponin, terpenoids, phenolic compounds and alkaloids. The methanolic extract of the fruits extracted from B. aegyptiaca exhibited a strong antioxidant activity in the DPPH assay and a potent capacity in preventing linoleic acid oxidation. Methanol extracts (50 and 100 mg/ml) were tested on several microbial strains using agar-well diffusion, minimum inhibitory concentration (MIC), minimum bacterial concentration (MBC) and minimum fungal concentration (MFC) methods. These experiments were performed on ten bacterial strains (five Gram-positives and five Gram-negatives) and four fungal strains. Methanol extracts, particularly at concentration of 100 mg/ml was found to be active against all bacterial and fungal strains and it was comparable to standard antibiotics Gentamicin and Amphotericin B. These findings support some of the traditional applications of the fruit of B. aegyptiaca against microbial aliments. It is therefore recommended that further studies regarding fractionation, separation and purification of these active antimicrobial compounds are required, in addition to toxicological evaluation in vivo.Key words: Balaneties aegyptiaca, antibacterial, antifungal, phytochemical, antioxidant, methanol extract

Efficacy of Pelargonium graveolens essential oils against some postharvest fungal diseases of apple

Background: Postharvest diseases that occur in apples are often caused by various pathogenic fungi, causing serious economic loss. The pathogenic fungi Penicillium expansum, Rhizopus stolonifer and Botrytis cinerea are among the most common pathogens in apples. The goal of this study was to see whether Moroccan Pelargonium graveolens essential oil (PGEO) could protect apple fruits from fungal infections after they were harvested (in vitro).Methods: PGEO was characterized by GC-MS and for antifungal assessment, in vitro poisoned food (PF) and volatile activity testing (VA) were carried out.Results: The investigation revealed that PGEO was effective against the three tested phytopathogenic fungi in a dose-dependent manner and this antifungal activity increased with the volatile activity test. The MIC value was 2 µL/mL for B. cinera, and R. stolonifer, and 1 µL/mL for P. expansum. Volatile fraction stops the growth of B. cinera at 40 µL / disc (QMI = 40 µL / disc), and of P. expansum and R. stolonifer at 80 µL / disc (QMI = 80 µL / disc).Conclusion: The current findings show that Moroccan PGEO has powerful antifungal activities, suggesting that it might be used instead of synthetic fungicides to combat apple post-harvest infections.Keywords: Pelargonium graveolens; Antifungal activity; Essential oil; Poisoned food; Volatile activity test; Apple

The global, regional, and national burden of adult lip, oral, and pharyngeal cancer in 204 countries and territories:A systematic analysis for the Global Burden of Disease Study 2019

Importance Lip, oral, and pharyngeal cancers are important contributors to cancer burden worldwide, and a comprehensive evaluation of their burden globally, regionally, and nationally is crucial for effective policy planning.Objective To analyze the total and risk-attributable burden of lip and oral cavity cancer (LOC) and other pharyngeal cancer (OPC) for 204 countries and territories and by Socio-demographic Index (SDI) using 2019 Global Burden of Diseases, Injuries, and Risk Factors (GBD) Study estimates.Evidence Review The incidence, mortality, and disability-adjusted life years (DALYs) due to LOC and OPC from 1990 to 2019 were estimated using GBD 2019 methods. The GBD 2019 comparative risk assessment framework was used to estimate the proportion of deaths and DALYs for LOC and OPC attributable to smoking, tobacco, and alcohol consumption in 2019.Findings In 2019, 370 000 (95% uncertainty interval [UI], 338 000-401 000) cases and 199 000 (95% UI, 181 000-217 000) deaths for LOC and 167 000 (95% UI, 153 000-180 000) cases and 114 000 (95% UI, 103 000-126 000) deaths for OPC were estimated to occur globally, contributing 5.5 million (95% UI, 5.0-6.0 million) and 3.2 million (95% UI, 2.9-3.6 million) DALYs, respectively. From 1990 to 2019, low-middle and low SDI regions consistently showed the highest age-standardized mortality rates due to LOC and OPC, while the high SDI strata exhibited age-standardized incidence rates decreasing for LOC and increasing for OPC. Globally in 2019, smoking had the greatest contribution to risk-attributable OPC deaths for both sexes (55.8% [95% UI, 49.2%-62.0%] of all OPC deaths in male individuals and 17.4% [95% UI, 13.8%-21.2%] of all OPC deaths in female individuals). Smoking and alcohol both contributed to substantial LOC deaths globally among male individuals (42.3% [95% UI, 35.2%-48.6%] and 40.2% [95% UI, 33.3%-46.8%] of all risk-attributable cancer deaths, respectively), while chewing tobacco contributed to the greatest attributable LOC deaths among female individuals (27.6% [95% UI, 21.5%-33.8%]), driven by high risk-attributable burden in South and Southeast Asia.Conclusions and Relevance In this systematic analysis, disparities in LOC and OPC burden existed across the SDI spectrum, and a considerable percentage of burden was attributable to tobacco and alcohol use. These estimates can contribute to an understanding of the distribution and disparities in LOC and OPC burden globally and support cancer control planning efforts

Burnout among surgeons before and during the SARS-CoV-2 pandemic: an international survey

Background: SARS-CoV-2 pandemic has had many significant impacts within the surgical realm, and surgeons have been obligated to reconsider almost every aspect of daily clinical practice. Methods: This is a cross-sectional study reported in compliance with the CHERRIES guidelines and conducted through an online platform from June 14th to July 15th, 2020. The primary outcome was the burden of burnout during the pandemic indicated by the validated Shirom-Melamed Burnout Measure. Results: Nine hundred fifty-four surgeons completed the survey. The median length of practice was 10 years; 78.2% included were male with a median age of 37 years old, 39.5% were consultants, 68.9% were general surgeons, and 55.7% were affiliated with an academic institution. Overall, there was a significant increase in the mean burnout score during the pandemic; longer years of practice and older age were significantly associated with less burnout. There were significant reductions in the median number of outpatient visits, operated cases, on-call hours, emergency visits, and research work, so, 48.2% of respondents felt that the training resources were insufficient. The majority (81.3%) of respondents reported that their hospitals were included in the management of COVID-19, 66.5% felt their roles had been minimized; 41% were asked to assist in non-surgical medical practices, and 37.6% of respondents were included in COVID-19 management. Conclusions: There was a significant burnout among trainees. Almost all aspects of clinical and research activities were affected with a significant reduction in the volume of research, outpatient clinic visits, surgical procedures, on-call hours, and emergency cases hindering the training. Trial registration: The study was registered on clicaltrials.gov "NCT04433286" on 16/06/2020

Assessing performance of the Healthcare Access and Quality Index, overall and by select age groups, for 204 countries and territories, 1990-2019: a systematic analysis from the Global Burden of Disease Study 2019

Background: Health-care needs change throughout the life course. It is thus crucial to assess whether health systems provide access to quality health care for all ages. Drawing from the Global Burden of Diseases, Injuries, and Risk Factors Study 2019 (GBD 2019), we measured the Healthcare Access and Quality (HAQ) Index overall and for select age groups in 204 locations from 1990 to 2019. Methods: We distinguished the overall HAQ Index (ages 0–74 years) from scores for select age groups: the young (ages 0–14 years), working (ages 15–64 years), and post-working (ages 65–74 years) groups. For GBD 2019, HAQ Index construction methods were updated to use the arithmetic mean of scaled mortality-to-incidence ratios (MIRs) and risk-standardised death rates (RSDRs) for 32 causes of death that should not occur in the presence of timely, quality health care. Across locations and years, MIRs and RSDRs were scaled from 0 (worst) to 100 (best) separately, putting the HAQ Index on a different relative scale for each age group. We estimated absolute convergence for each group on the basis of whether the HAQ Index grew faster in absolute terms between 1990 and 2019 in countries with lower 1990 HAQ Index scores than countries with higher 1990 HAQ Index scores and by Socio-demographic Index (SDI) quintile. SDI is a summary metric of overall development. Findings: Between 1990 and 2019, the HAQ Index increased overall (by 19·6 points, 95% uncertainty interval 17·9–21·3), as well as among the young (22·5, 19·9–24·7), working (17·2, 15·2–19·1), and post-working (15·1, 13·2–17·0) age groups. Large differences in HAQ Index scores were present across SDI levels in 2019, with the overall index ranging from 30·7 (28·6–33·0) on average in low-SDI countries to 83·4 (82·4–84·3) on average in high-SDI countries. Similarly large ranges between low-SDI and high-SDI countries, respectively, were estimated in the HAQ Index for the young (40·4–89·0), working (33·8–82·8), and post-working (30·4–79·1) groups. Absolute convergence in HAQ Index was estimated in the young group only. In contrast, divergence was estimated among the working and post-working groups, driven by slow progress in low-SDI countries. Interpretation: Although major gaps remain across levels of social and economic development, convergence in the young group is an encouraging sign of reduced disparities in health-care access and quality. However, divergence in the working and post-working groups indicates that health-care access and quality is lagging at lower levels of social and economic development. To meet the needs of ageing populations, health systems need to improve health-care access and quality for working-age adults and older populations while continuing to realise gains among the young. Funding: Bill & Melinda Gates Foundation