A new approach for achievement of inulin accumulation in suspension cultures of Jerusalem artichoke (Helianthus tuberosus) using biotic elicitors

AbstractA promising protocol for achievement the accumulation rate of inulin compound in a suspension culture of Jerusalem artichoke (Helianthus tuberosus) was established. The effect of incorporated of cell cultures in combining with two type of biotic elicitors Aspergillus niger extract and Methyl-Jasmonate incorporation feeding medium on leaf cell growth patterns and production of inulin was investigated. The maximum value of cell growth parameters and highest content of inulinase activity (0.395u/ml) were resulted from elicitation of augmented MS-medium with A. niger extract at the level of 0.2% in combination with Methyl-Jasmonate (150μM) as compared with other concentrations after 2weeks of cultivation. The chemical analyses of the different cell lines were spectro-photometerically performed. This study clearly indicates that combining of A. niger and Methyl-Jasmonate elicitors plays a critical role on inulin process and its accumulation in Jerusalem artichoke cell cultures

ANTI-TUMOR ACTIVITIES AND OIL CONSTITUENTS OF ZINGIBER OFFICINALE (ZINGIBERACEAE

The phytochemical studies on Zingiber officinale rhizomes revealed that it contains traces of flavonoid, carbohydrates, tannins, steroles or terpenoids and it is free from alkaloids.Assay of essential oil of Zingiber officinale rhizome was also carried out. The alcoholic extract of the plant rhizome, was examined against five tumor cell lines, BHK-2, HEPG-2, MCF-7, HCT-2 and HEP-2, using SRB. assay. All cell lines were growth inhibited in a dose dependent manner after exposure to the plant extract. The antitumor activity of the plant extract using E.A.C. method, showed a high activity against mice tumor

Karyotype analysis for date palm (Phoenix dactylifera L) compared with tissue culture derived plants

Abstract: The cytological studies on the micropropagated date palm (phoenix dactylifera var. Karama) revealed that no morphological changes occurred during microproopagation and there were 36 chromosomes arranged in 18 bivalents of chromosomes in c-meta phase profile, seventeen bivalents are autosomal chromosomes and XY bivalent in male or XX in female. Chromosome No.2, 3, 4, 5 and 6 were meta centric, chromosomes No. 1,7,8,9 and 10 were submetacentric and chromosomes from No.11 to No17 were subtelocentric. While X chromosome was submetacentric and Y chromosome was subtelocentric. There were no significant differences in the chromosome length, area and centromer positions between the micropropagated plantlets and the mother plants. [Mohamed M. AbdAlla¹ and Aesha M. Abd El-Kawy. Karyotype analysis for date palm (Phoenix dactylifera L) compared with tissue culture derived plants

ORIGINAL ARTICLES Effect of Heavy Metal (HgCl 2 ) on Accumulation and Production of Total Indole Alkaloids, Vinblastine And/or Vincristine from Egyptian Catharanthus Roseus

ABSTRACT Catharanthus roseus is still the only source for the powerful antitumor drugs vinblastine and vincristine. Effects of mercuric (HgCl 2 ) on enhancement the accumulation rate of total indole alkaloids; antineoplastic agents (vinblastine and/or vincristine) in calli cultures of Egyptian Catharanthus roseus (L.) G. (Don) were studied. Hgcl 2 was used at levels of 0.0, 0.2, 0.4 and 0.8 μm/l, added to modified alkaloid production medium (MAPM). The obtained results showed that: All tested concentrations of Hg significantly decreased the different measured growth parameters of shoot and root derived calli, however, the percentage of dry matter content significantly increased comparing the control treatment. Moreover, the percentage of total indole-alkaloids was significantly increased. The highest values of the accumulation rate of total indole-alkaloids, further, vinblastine and/or vincristine were recorded at the rate of 0.8 mM / Hg in shoot and root derived calli, respectively

Determination of potentially irrigable agricultural lands using remote sensing and geographic information system: case study of Yamula Basin

Yamula Dam that has been constructed in Kayseri is one of the most important projects regarding the irrigation of agricultural lands. The total area of the collected water body was reached in 2005 as planned before, and electricity production started. Together with the realization of the project, it is planned to use agricultural lands in basin more efficiently and productively. In this study, it was aimed to determine the hydrological structure of Yamula Basin and potentially irrigable lands by using geographic information systems and remote sensing technologies. The hydrological structure was determined using digital elevation model. The land use map was prepared by using Landsat satellite image for the year 2016. The analysis and queries were carried out by overlapping the land use, land use capacity, topographic maps and sub-basin layers. The potential agricultural lands were determined in accordance with the results obtained from the spatial analysis