MonoMAC Syndrome Caused by a Novel GATA2 Mutation Successfully Treated by Allogeneic Hematopoietic Stem Cell Transplantation

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Cine y ciencia: un análisis de los estereotipos presentes en la película infantil Frankenweenie, de Tim Burton

Este artigo tem como objetivo analisar, à luz dos estudos culturais, como os processos relacionados à marcação das identidades de gênero e reforço de estereótipos associados com cientistas e prática científica estão presentes no filme Frankenweenie, produzido pela Disney e dirigido por Tim Burton. A metodologia aplicada a este artigo foi a análise de conteúdo, método que se caracteriza pela utilização de um conjunto de técnicas que tentam descrever e interpretar o conteúdo de comunicação. Os resultados apontam alguns reforços de estereótipo quanto ao gênero e à imagem solitária e egocêntrica do cientista, bem como a ausência de uma visão crítica da ciência do cientista na contemporaneidade. Este estudo permitirá o cruzamento de discursos sobre a ciência no campo da divulgação científica, mostrando que a produção cinematográfica pode ser uma excelente fonte de pesquisa para os estudos culturais da ciência. Acredita-se que análises deste tipo podem levantar discussões sobre como o cinema pode ser usado em benefício do desenvolvimento do senso crítico das crianças e do aprendizado sobre como compreender e resignificar o mundo que as cerca

Inflamación crónica granulomatosa en el pez teleósteo Piaractus mesopotamicus: modelo de estudio histopatológico

Objective. This study evaluated the cell kinetic and formation of granuloma during chronic inflammation induced by Bacillus Calmette-Guérin (BCG) in the skeletal muscle of Piaractus mesopotamicus, as a histopathology model to study innate immunity. Materials and methods. Sixty fish were divided in two groups: BCG-inoculated and non-inoculated fish and the inflammatory response analyzed 3, 7, 14, 21 and 33 days post-inoculation (DPI) by histopathology after hematoxylin-eosin and Ziehl-Neelsen staining. Results. 3 DPI of BCG showed a diffuse inflammatory reaction mostly composed by mononuclear cells. The inflammation continued diffuse 7 DPI initiating the cellular organization surrounding the inoculum and have continued at 14 DPI with discrete presence of epithelioid-like type cells with acidophilic cytoplasm and floppy chromatin. Higher cellular organization (21 DPI) surrounding the granuloma with intense peripheral mononuclear inflammatory infiltrate and nevertheless, an increase in the number of fibroblasts and macrophage-like cells was observed. The inflammatory process became less diffuse 33 DPI with formation of small amount of granuloma surrounded by the same type of reaction found in bigger granuloma. Both the young and old granuloma presented typical characteristic around the inoculum composed by a layer of epithelioid-like type cells, besides macrophages, some lymphocytes and abundant fibroblasts. Conclusions. This study showed the feasibility in the use of pacus to study chronic granulomatous inflammatory response induced by BCG, characterized by changes in the kinetics of inflammatory cells in skeletal muscle classifying as immune-epithelioid type, similar to granulomatous inflammation caused by M. marinum in teleost fish.Objetivo. Este estudio evaluó la cinética celular y la formación de granuloma durante la inflamación crónica inducida por el Bacilo Calmette-Guérin (BCG) en el músculo esquelético de Piaractus mesopotamicus, como modelo histopatológico para estudiar la inmunidad innata. Materiales y métodos. Sesenta peces fueron divididos en dos grupos: peces inoculados con BCG y no inoculados y la respuesta inflamatoria analizada en 3, 7, 14, 21 y 33 días post-inóculo (DPI) por medio del análisis histopatológico y tinciones de hematoxilina-eosina y Ziehl-Neelsen. Resultados. 3 DPI de BCG se observó reacción inflamatoria difusa principalmente formada por infiltrado celular mononuclear. Al 7° DPI la inflamación continuaba difusa con inicio de organización celular alrededor del inoculo, que se observó hasta el 14° DPI con discreta presencia de células de tipo epiteliodes con citoplasma acidófilo y cromatina laxa. Para el 21° DPI se observó alta organización celular alrededor del granuloma con intenso infiltrado mononuclear periférico e incremento en el número de fibroblastos y macrófagos. El proceso inflamatorio se tornó menos difuso a los 33 DPI con formación de pequeños granulomas contenidos dentro de uno más grande. Los granulomas formados más rápidamente así como los formados tardíamente, presentaron características típicas alrededor del inóculo compuesta por una camada de células tipo epitelioides, macrófagos, linfocitos y fibroblastos. Conclusiones. Este estudio mostró la viabilidad del uso del P. mesopotamicus para estudiar la respuesta inflamatoria crónica granulomatosa inducida con BCG, caracterizado por la evolución de la cinética de células inflamatorias en el músculo esquelético clasificándolo como de tipo inmune-epitelioide, similar a la inflamación granulomatosa causada por M. marinum en peces teleósteos

Exploring Cluster Analysis in Nelore Cattle Visual Score Attribution

Assessing the biotype of cattle through human visual inspection is a very common and important practice in precision cattle breeding. This paper presents the results of a correlation analysis between scores produced by humans for Nelore cattle and a variety of measurements that can be derived from images or other instruments. It also presents a study using the k-means algorithm to generate new ways of clustering a batch of cattle using the measurements that most correlate with the animal's body weight and visual scores

Lectinhistochemical staining of granuloma induced by bacillus Calmette-Guerin in Piaractus mesopotamicus

ABSTRACT Objetive. This study was conducted to evaluate, by means of lectinhistochemistry (LHC), the expression of carbohydrates in granulomas induced by the bacillus Calmette-Guerin (BCG) in muscle tissue of Piaractus mesopotamicus after 33 days. Material and methods. Histological sections with 3 μm thick were incubated with the following lectins :WGA (Wheat germ agglutinin), DBA (Dolichos biflorus agglutinin) and HPA (Helix pomatia agglutinin), and the results were evaluated by light microscopy. Results. Acid fast bacilli were stained by Ziehl Neelsen (ZN) and strong labeled by WGA in the cytoplasm of macrophages. Labeling with DBA was intense in fibroblasts and weak in macrophages. On the other hand, HPA binding was stronger in macrophages, especially in those that were in close contact with epithelioid cells, without evidence of binding to fibroblasts. The epithelioid cells were not labeled by the used lectins, but they were identified by Hematoxilin-Eosin (HE). The lectins labeled specific type saccharides in glycoproteins, as N-acetylglucosamine present in bacilli and macrophages, as well as N-acetyl-galactosamine in macrophages. The control group showed no inflammation or lectin binding. Conclusions. This technique may be useful in identifying receptors for WGA, DBA and the HPA lectins in epithelioid granuloma induced by BCG in P. mesopotamicu

Structural basis for the RING catalyzed synthesis of K63 linked ubiquitin chains

This work was supported by grants from Cancer Research UK (C434/A13067), the Wellcome Trust (098391/Z/12/Z) and Biotechnology and Biological Sciences Research Council (BB/J016004/1).The RING E3 ligase catalysed formation of lysine 63 linked ubiquitin chains by the Ube2V2–Ubc13 E2 complex is required for many important biological processes. Here we report the structure of the RING domain dimer of rat RNF4 in complex with a human Ubc13~Ub conjugate and Ube2V2. The structure has captured Ube2V2 bound to the acceptor (priming) ubiquitin with Lys63 in a position that could lead to attack on the linkage between the donor (second) ubiquitin and Ubc13 that is held in the active “folded back” conformation by the RING domain of RNF4. The interfaces identified in the structure were verified by in vitro ubiquitination assays of site directed mutants. This represents the first view of the synthesis of Lys63 linked ubiquitin chains in which both substrate ubiquitin and ubiquitin-loaded E2 are juxtaposed to allow E3 ligase mediated catalysis.PostprintPeer reviewe

Técnica da complementação de frases como recurso para expressão vivencial do aluno em véspera de prova

This article evaluates an alternative strategy of group intervention using the technique of complementing sentences to a group of students at University just before their examination. It aimed at offering to the nursing undergraduate students from the College of Nursing at Ribeirão Preto - University of São Paulo, a space to show their feelings related to this peculiar situation. The authors searched to catch the students' emotional conditions regarding their expectations as well as to identify possible aspects that can be handled looking at suggesting alternatives of intervention to alleviated tension.El presente artículo, evalúa la estrategia alternativa de la intervención en grupo mediante la técnica de la complementación de frases en Universitarios, en el día anterior de a pruebas de conocimientos. Tuvo como objetivos: a) ofrecer a los alumnos del curso de pre-grado en enfermería de la Escuela de Enfermería de Ribeirao Preto - USP, una posibilidad de expresión vivencial en situación específica; b) captar sus condiciones emocionales relacionadas a sus expectativas; c) identificar aspectos posibles de ser manejados y; de sugerir una posible alternativa de intervención para alivio de sus tensiones.O presente artigo avalia a estratégia alternativa de integração em grupo por meio da aplicação da técnica da complementação de frases em Universitários, em véspera de prova. Teve como objetivo: a) oferecer aos alunos do curso de graduação em enfermagem da Escola de Enfermagem de Ribeirão Preto da Universidade de São Paulo, uma possibilidade de expressão vivencial da situação peculiar; b) apreender suas condições emocionais relacionadas às suas expectativas; c) identificar aspectos possíveis de serem manejados e; d) sugerir uma possível alternativa de intervenção para alívio de tensões