A efetividade do laser de HeNe 632,8 nm no reestabelecimento da integridade dos tecidos cutâneos em animais experimentais: revisão sistemática

O objetivo desta revisão sistemática foi analisar o efeito do laser de HeNe na cicatrização de feridas em ratos. Foram selecionados estudos experimentais que adotaram o laser HeNe para o tratamento de feridas agudas em ratos adultos saudáveis, com lesões induzidas por bisturi, nas bases de dados PubMed/MEDLINE, LILACS e SciELO. Foram utilizados os seguintes descritores: cicatrização de feridas e colágeno, de acordo com o MeSH e o DeCS, além dos unitermos laser HeNe e reparação da pele e seus equivalentes em inglês e espanhol. Três estudos foram incluídos na revisão sistemática, não sendo possível a realização de metanálise, devido à impossibilidade de comparação entre as metodologias dos estudos selecionados. Todos os estudos realizaram análise por meio de cortes histológicos das cicatrizes. A presença de falhas metodológicas nos três artigos dificultou a interpretação fidedigna dos dados encontrados. Os estudos destacaram uma redução na intensidade da resposta inflamatória e uma melhor organização das fibras colágenas no grupo irradiado. A terapia com laser HeNe mostrou boa resposta no reparo tecidual. No entanto, tais resultados devem ser analisados de modo criterioso, uma vez que há presença de heterogeneidade, principalmente em relação aos parâmetros adotados

Genotoxic potential generated by biomass burning in the Brazilian Legal Amazon by Tradescantia micronucleus bioassay: a toxicity assessment study

<p>Abstract</p> <p>Background</p> <p>The Brazilian Amazon has suffered impacts from non-sustainable economic development, especially owing to the expansion of agricultural commodities into forest areas. The Tangará da Serra region, located in the southern of the Legal Amazon, is characterized by non-mechanized sugar cane production. In addition, it lies on the dispersion path of the pollution plume generated by biomass burning. The aim of this study was to assess the genotoxic potential of the atmosphere in the Tangará da Serra region, using <it>Tradescantia pallida </it>as <it>in situ </it>bioindicator.</p> <p>Methods</p> <p>The study was conducted during the dry and rainy seasons, where the plants were exposed to two types of exposure, active and passive.</p> <p>Results</p> <p>The results showed that in all the sampling seasons, irrespective of exposure type, there was an increase in micronucleus frequency, compared to control and that it was statistically significant in the dry season. A strong and significant relationship was also observed between the increase in micronucleus incidence and the rise in fine particulate matter, and hospital morbidity from respiratory diseases in children.</p> <p>Conclusions</p> <p>Based on the results, we demonstrated that pollutants generated by biomass burning in the Brazilian Amazon can induce genetic damage in test plants that was more prominent during dry season, and correlated with the level of particulates and elevated respiratory morbidity.</p

Antinociceptive action and redox properties of citronellal, an essential oil present in lemongrass

This is a copy of an article published in the Journal of Medicinal Food© 2011 - copyright Mary Ann Liebert, Inc.; Journal of Medicinal Food is available online at: http://online.liebertpub.comCitronellal (CT) is a monoterpenoid and the major constituent of the mixture of terpenoids that give the citronella oil its lemon scent. Citronella oil is widely used around the world for various purposes and is mainly obtained from plants of the Cymbopogon genus, which are known as “lemongrass.” Considering these plants have been used worldwide for various medicinal purposes, the interest of researchers to understand the biological activities of monoterpenoids related to the Cymbopogon genus has been increasing. In the present work, we investigated the antinociceptive action and the redox properties of CT. Our results indicate that intraperitoneal injection of CT was effective in reducing nociceptive face-rubbing behavior in both phases of the formalin test, which was also naloxone-sensitive. CT also evoked antinociceptive response in the capsaicin and glutamate tests. The total radical-trapping antioxidant parameter and total antioxidant reactivity assays indicate that CT at doses of 0.1 and 1 mg/mL exerts a significant antioxidant activity, which is probably related to its ability to scavenge superoxide and nitric oxide, but not H2O2 or hydroxyl radicals, as evaluated separately by specific in vitro tests. These results show for the first time the antinociceptive potential of CT and indicate that the antioxidant properties of this compound may rely on its mechanism of biological actions because CT-containing natural products are used to treat various diseases related to oxidative stress and reactive species

2 nd Brazilian Consensus on Chagas Disease, 2015

Abstract Chagas disease is a neglected chronic condition with a high burden of morbidity and mortality. It has considerable psychological, social, and economic impacts. The disease represents a significant public health issue in Brazil, with different regional patterns. This document presents the evidence that resulted in the Brazilian Consensus on Chagas Disease. The objective was to review and standardize strategies for diagnosis, treatment, prevention, and control of Chagas disease in the country, based on the available scientific evidence. The consensus is based on the articulation and strategic contribution of renowned Brazilian experts with knowledge and experience on various aspects of the disease. It is the result of a close collaboration between the Brazilian Society of Tropical Medicine and the Ministry of Health. It is hoped that this document will strengthen the development of integrated actions against Chagas disease in the country, focusing on epidemiology, management, comprehensive care (including families and communities), communication, information, education, and research