A review of bioanalytical techniques for evaluation of cannabis (Marijuana, weed, Hashish) in human hair

Cannabis products (marijuana, weed, hashish) are among the most widely abused psychoactive drugs in the world, due to their euphorigenic and anxiolytic properties. Recently, hair analysis is of great interest in analytical, clinical, and forensic sciences due to its non-invasiveness, negligible risk of infection and tampering, facile storage, and a wider window of detection. Hair analysis is now widely accepted as evidence in courts around the world. Hair analysis is very feasible to complement saliva, blood tests, and urinalysis. In this review, we have focused on state of the art in hair analysis of cannabis with particular attention to hair sample preparation for cannabis analysis involving pulverization, extraction and screening techniques followed by confirmatory tests (e.g., GC–MS and LC–MS/MS). We have reviewed the literature for the past 10 years’ period with special emphasis on cannabis quantification using mass spectrometry. The pros and cons of all the published methods have also been discussed along with the prospective future of cannabis analysis

Recruitment of a genotyped Quercus robur L. seedling cohort in an expanding oak forest stand: diversity, dispersal, and performance across habitats

Key message : Few studies have linked the origin of dispersed tree seeds with their post-dispersal fate. We show that habitat-dependent mortality in a pedunculate oak (Quercus roburL.) seedling cohort reshapes the effective fecundity of individual mother trees but has little effect on the cohort's genetic diversity. Context : Initial tree recruitment plays a key role in forest regeneration, yet little is known on how patterns of recruit mortality feed back on the fecundity of reproducing trees. Aims : To investigate how among-habitat variation in seedling arrival and survival alters initial patterns of genetic diversity and maternal reproductive success. Methods : We genotyped a pedunculate oak seedling cohort (n = 809) and monitored it over 3 years. The mother trees of 81% of the seedlings were identified through parentage analysis. Seedlings were assigned to one of three habitats (broadleaved forest, pine plantation, or open area). Results : Broadleaved forest received most seedlings (approximate to 65%) but their survival was reduced by a third compared with pine plantations or open areas. Thus, mother trees dispersing many descendants to broadleaved forest suffered a disproportionate reduction of their reproductive success. Genetic diversity did not vary among habitats, nor over the monitoring period. Conclusion : The quality of seed dispersal, in terms of delivery sites, can considerably influence the reproductive success of individual mother trees without affecting the overall genetic diversity of the recruits.PATRONS SPATIO-TEMPORELS DE COLONISATION DANS UNE POPULATION D'ARBRES EN EXPANSION: une approche integrant génétique et génomiqu

Associational resistance to a pest insect fades with time

Tree diversity is one of the drivers of forest resistance to herbivores. Most of the current understanding of the diversity resistance relationship comes primarily from short-term studies. Knowing whether tree diversity effects on herbivores are maintained over time is important for perennial ecosystems like forests. We addressed the temporal dynamics of the diversity resistance relationship by conducting a 6-year survey of pine attacks by the pine processionary moth Thaumetopoea pityocampa (PPM) in a tree diversity experiment where we could disentangle tree composition from host density effects. During the first years after planting the trees, PPM attacks on maritime pine Pinus pinaster were reduced in the presence of birch Betula pendula, a fast-growing non-host tree (i.e. associational resistance). This effect was maintained but faded with time as the pines eventually grew taller than neighbouring birches. The number of repeated attacks on individual pine trees also decreased in mixed pine-birch stands. Pine density had a positive effect on stand colonisation by PPM and a negative effect on the proportion of trees that were attacked. Pines were less likely to be repeatedly attacked as pine density increased, with attacks being spread over a larger number of host trees. Collectively, these results unravel the independent contribution of tree species composition and host density to tree resistance to herbivores. Both processes had directional changes over time. These results indicate that the resistance of planted forests to herbivores can be improved by carefully choosing the composition of mixed forests and the timing of species planting

Insect – Tree Interactions in Thaumetopoea pityocampa

The pine processionary moth is, by far, the most important insect defoliator of pine forests in Southern Europe and North Africa, both in terms of its temporal occurrence, geographic range and socioeconomic impact. Monitoring and pest management actions are therefore required on a regular basis, to ensure the detection, evaluation and mitigation of potential risks to forest and public health. However, we still lack some of the basic knowledge required for relevant analyses of the risk posed by the pine processionary moth. Pest risk is defined as a combination of three components: (1) pest occurrence, which depends on the spatiotemporal dynamics of populations; (2) plant vulnerability to the pest, resulting in a certain amount of damage; and (3) the socioeconomic impact of damage, depending on the potential value of the plants damaged (Jactel et al. 2012). The population dynamics of the processionary moth has been extensively studied, in particular within the context of climate change (see Battisti et al. 2014, Chap. 2, this volume). Several studies have recently addressed the question of tree and forest vulnerability to pine processionary attacks but a comprehensive review of evidence was missing. This is the first objective of this chapter. In particular we were interested in a better understanding of the ecological mechanisms responsible for the host tree selection, at both the species and individual tree levels. In a second part we show that pine susceptibility to the pine processionary moth could be reduced by improving forest diversity at different spatial scales. In the last part of this chapter we provide quantitative estimate of the growth losses caused by defoliations of the pine processionary moth. Altogether this information paves the way for quantitative risk analyses on pine processionary moth infestations based on forest growth models