Effect of Quercetin on Preosteoblasts and Bone Defects

Quercetin is a flavonol, also a phytoestrogen, available commonly in onion and apple. Our laboratory investigated its effect on MC3T3-E1 cells’ alkaline phosphatase activity in vitro and compared the amount of new bone produced by quercetin in collagen matrix to that produced by bone grafts and collagen matrix in vivo. Four bone defects, 5mm by 10mm were created in the parietal bone of 2 New Zealand White rabbits. In the experimental animal, 2 defects were grafted with quercetin solution mixed with collagen matrix. In the control animal, 2 defects were grafted with collagen matrix alone. Animals were killed on day 14 and the defects were dissected and prepared for histological qualitative assessment. Results showed that 10μM of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells at 72 hours in vitro by 32%. In the experimental animal, there was new bone growing inside the bone defects. In conclusion, specific concentration of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells in vitro and quercetin in collagen matrix has the effect of forming new bone across bone defects in vivo

Local Massage with Topical Analgesic, a Novel Treatment Modality for Temporomandibular Muscular Pain, a Case Study Report of 5 Consecutive Cases

INTRODUCTION: Temporomandibular disorders (TMDs) represent a group of painful conditions involving the muscles of mastication and the temporomandibular joint. Ping On Ointment has been used in the Chinese Orthopedics as a soothing massage balm for muscular aches, strain and sprain. If topical application of the ointment can be effective for the treatment of TMD muscular pain, it may be the long-sought-after method for safe, simple, cheap, non-invasive, and effective treatment modality of TMD muscular pain. PURPOSE: This report documented a case study of the first five consecutive cases using this treatment modality. RESULTS: All cases resulted in complete remission of pain within one month of topical massage. CONCLUSION: This treatment method has high potential to benefit a significant number of people and randomized control trials should be performed.published_or_final_versio

Effect of Bio-Oss® Collagen and Collagen Matrix on Bone Formation

Objective: to compare the amount of new bone produced by Bio-Oss ® Collagen to that produced by collagen matrix in vivo. Method: eighteen bone defects, 5mm by 10mm were created in the parietal bone of 9 New Zealand White rabbits. 6 defects were grafted with Bio-Oss ® Collagen. 6 defects were grafted with collagen matrix alone (positive control) and 6 were left empty (negative control). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Quantitative analysis of new bone formation was made on 100 sections (50 sections for each group) using image analysis. Results: A total of 339% more new bone was present in defects grafted with Bio-Oss ® Collagen than those grafted with collagen matrix (positive control). No bone was formed in the negative control group. Conclusion: Bio-Oss ® Collagen has the effect of stimulating new bone formation locally compared with collagen matrix in vivo. Bio-Oss ® Collagen may be utilized as a bone graft material. © Wong and Rabie; Licensee Bentham Open.published_or_final_versio

Repositioning of the gingival margin by extrusion

10.1067/mod.2002.122397American Journal of Orthodontics and Dentofacial Orthopedics122195-102AJOO