Effect of organically and conventionally produced diets on jejunal gene expression in chickens

Using a nutrigenomics approach we studied the response of second-generation chickens at a transcriptional level to organically grown feed ingredients compared with conventionally grown feed ingredients. Both diets consisted of the same amounts of ingredients, the only difference was the production method. Gene expression was analysed in jejuni using whole genome chicken cDNA arrays. After analysis, forty-nine genes were found to be differentially regulated between chickens fed on the different diets, independent of their genetic background. Of these forty-nine genes, seven genes were involved in cholesterol biosynthesis. Genes involved in cholesterol biosynthesis were higher expressed in jejuni from organically fed birds. Other genes found to be regulated were involved in immunological processes, such as B-G protein (part of chicken major histocompatibility complex), chemokine ah221, and the immunoglobulin heavy chain. Using quantitative PCR the effect of genetic background on the differential expression of genes was studied. Differences in gene expression existed between animals fed different diets as well as between different chicken lines. This indicated that diet and genetic background influence the transcriptional response of the jejunum. This is the first time that significant differences in gene expression were shown between animals on diets with organically or conventionally produced ingredient

Quantitative relationships between benthic diatom assemblages and water chemistry in Macquarie Island lakes and their potential for reconstructing past environmental changes

This study is the first published survey of diatom-environment relationships Oil sub-Antarctic Macquarie Island. Fifty-eight sites in 50 coastal and inland lakes were sampled for benthic diatoms and water chemistry. 208 diatom species from 34 genera were identified. Multivariate analyses indicated that the lakes were distributed along nutrient and conductivity gradients. Conductivity, pH, phosphate (SRP), silicate and temperature all explained independent portions of the variance in the diatom data. Transfer functions provide a quantitative basis for palaeolimnological studies of past climate change and human impacts, and can be used to establish baseline conditions for assessing the impacts of recent climate change and the introduction of non-native plants and animals. Statistically robust diatom transfer functions for conductivity, phosphate and silicate were developed, while pH and temperature transfer functions performed less well. The lower predictive abilities of the pH and temperature transfer functions probably reflect the broad pH tolerance range of diatoms on Macquarie Island and uneven distribution of lakes along the temperature gradient. This study contributes to understanding the current ecological distribution of Macquarie Island diatoms and provides transfer functions that will be applied in studies of diatoms in lake sediment cores to quantitatively reconstruct past environmental changes

Differential Item Functioning and educational risk factors in Guatemalan reading assessment

Examinamos indicadores de Funcionamiento Diferencial de Ítemes (FDI) asociados a cuatro variables que han demostrado de manera repetida ser factores de riesgo para el logro escolar. Estos factores son el sobre-edad para el grado de matriculación, área de residencia urbana/rural, etnia y género. Para este estudio utilizamos los datos de las evaluaciones nacionales del tercer grado. Dado que en la literatura se reporta con frecuencia que los indicadores de FDI son inestables, utilizamos tres diferentes métodos para estimarlo (chi-cuadrado, Rasch, regresión logística) y evaluamos su consistencia en datos de tres diferentes años de evaluaciones. Encontramos evidencia de FDI. Sin embargo, la eliminación de ítemes con FDI no cambió las diferencias entre grupos que se encontraron en las puntuaciones de las evaluaciones. Los hallazgos sugieren que los factores de riesgo educativo actúan de manera conjunta en esta población guatemalteca y que hay alguna interacción entre estos factores de riesgo para generar sesgo. Concluimos que será de beneficio tomar en cuenta múltiples variables de contexto asociadas al riesgo educativo de forma simultanea al analizar FDI y al desarrollar evaluaciones

Pitch contours of Northern Vietnamese tones vary with focus marking

Intonation is a means of structuring discourse and one of its functions is to highlight new or contrasting information, i.e., focus. Speakers of different languages use a range of prosodic cues to mark focus. Compared to non-tonal languages such as English, tonal languages use pitch to distinguish lexical tones and focus marking. Determining the interplay between intonation and lexical tone is therefore important. Previous studies found that tonal languages use different strategies to mark focus. For example, some use an increase (e.g., Mandarin Chinese), others a decrease in pitch (e.g., Kammu). The Vietnamese language has six lexical tones and is particularly interesting for examining pitch contours in focus marking. In this article, we present a production study with 70 Northern Vietnamese speakers. Participants read six sentences aloud under two different conditions (narrow/wide focus). In each sentence, focus marked a single noun (‘focus item’) which occurred in the final position of the sentence and carried one of the six tones. Acoustic analyses of the focus item showed that Vietnamese speakers realized focus with significant differences in pitch at the beginning of the word, but the strategies to increase or decrease pitch varied across tones. Our findings add important insights to the discussion about Information Structure and the role of intonation in tonal languages by analyzing the use of prosodic cues in a complex tone system. The large number of speakers in our study also adds further methodological rigor compared to other studies, which often rely on a few speakers.1 Introduction 2 Using intonation for focus marking 2.1 Focus marking with intonation in tonal languages 2.2 Using intonation to mark pragmatic functions and focus in Vietnamese 3 Method 3.1 Participants 3.3 Material 3.2 Procedure 3.3 Pitch Analysis 4 Results 5 Discussio