Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity

Genomic maps of chromatin modifications have provided evidence for the partitioning of genomes into domains of distinct chromatin states, which assist coordinated gene regulation. The maintenance of chromatin domain integrity can require the setting of boundaries. The HS4 insulator element marks the 3′ boundary of a heterochromatin region located upstream of the chicken β-globin gene cluster. Here we show that HS4 recruits the E3 ligase RNF20/BRE1A to mediate H2B mono-ubiquitination (H2Bub1) at this insulator. Knockdown experiments show that RNF20 is required for H2Bub1 and processive H3K4 methylation. Depletion of RNF20 results in a collapse of the active histone modification signature at the HS4 chromatin boundary, where H2Bub1, H3K4 methylation, and hyperacetylation of H3, H4, and H2A.Z are rapidly lost. A remarkably similar set of events occurs at the HSA/HSB regulatory elements of the FOLR1 gene, which mark the 5′ boundary of the same heterochromatin region. We find that persistent H2Bub1 at the HSA/HSB and HS4 elements is required for chromatin boundary integrity. The loss of boundary function leads to the sequential spreading of H3K9me2, H3K9me3, and H4K20me3 over the entire 50 kb FOLR1 and β-globin region and silencing of FOLR1 expression. These findings show that the HSA/HSB and HS4 boundary elements direct a cascade of active histone modifications that defend the FOLR1 and β-globin gene loci from the pervasive encroachment of an adjacent heterochromatin domain. We propose that many gene loci employ H2Bub1-dependent boundaries to prevent heterochromatin spreading

Radioisotope thermionic power supply for spacecraft

Power supply design for unmanned electric propulsion missions to outer planets utilizes a store of curium-244 in compact array of capsules as energy source. Supply subassemblies are: heat source, converter equipment which supplies power, and safety equipment. System is designed for a 72,000 hour mission

Thermionic reactor power system: Effects of radiation on integration with Manned Space Station

The application of a thermionic reactor power system to the modular space station is described. The nominal net power is 40 kWe, with the power system designed to be applicable over the power range from 25 to 60 kWe. The power system is designed to be launched by the space shuttle. Radiation protection is provided by LiH neutron shielding and W gamma shielding in a shaped 4 pion configuration, i.e., the reactor is shielded on all sides but not to equal extent. Isodose contours are presented for the region around the modular space station. Levels and spectral distribution of radiation are given for later evaluation of effects on space station experiments. Parametric data on the effects of separation distance on power system mass are presented

Nanocrystal seeding: A low temperature route to polycrystalline Si films

A novel method is presented for growth of polycrystalline silicon films on amorphous substrates at temperatures of 540–575 °C. Grain nucleation and grain growth are performed in two steps, using Si nanocrystals as nuclei ("seeds"). The nanocrystal seeds are produced by excimer laser photolysis of disilane in a room temperature flow cell. Film (grain) growth occurs epitaxially on the seeds in a separate thermal chemical vapor deposition (CVD) step, with growth rates 10–100 times higher than similar CVD growth rates on crystal Si. Grain size and CVD growth rates are dependent on seed coverage, for seed coverage <0.2 monolayers

Permutation groups, simple groups and sieve methods

We show that the number of integers n ≤ x which occur as indices of subgroups of nonabelian finite simple groups, excluding that of An-1 in An, is ∼ hx/log x, for some given constant h. This might be regarded as a noncommutative analogue of the Prime Number Theorem (which counts indices n ≤ x of subgroups of abelian simple groups). We conclude that for most positive integers n, the only quasiprimitive permutation groups of degree n are Sn and An in their natural action. This extends a similar result for primitive permutation groups obtained by Cameron, Neumann and Teague in 1982. Our proof combines group-theoretic and number-theoretic methods. In particular, we use the classification of finite simple groups, and we also apply sieve methods to estimate the size of some interesting sets of primes

Letter to the Editor: a response to Horne and Lucey (2017)

No abstract available

The control of a nuclear reactor using helium- 3 gas control elements

Control system for water moderated reactor using helium-3 ga

Variations in the stratospheric ozone field inferred from Nimbus satellite observations

The ultraviolet earth radiance data from the Backscatter Ultraviolet Experiment on Nimbus 4 have been inverted to infer ozone profiles using a single Rayleigh scattering model. Two methods of solution give essentially the same results. Comparisons of these profiles with simultaneous rocket sounding data shows satisfactory agreement at low and middle latitudes. Vertical cross sections of ozone mixing ratio along the orbital tracks indicate that while the gross characteristics of the ozone field above 10 mb are under photochemical control, the influence of atmospheric motions can be found up to the 4 mb level