10 research outputs found
Cloning and expression in Escherichia coli of full-length complementary DNA coding for human α1-antitrypsin
A cDNA library prepared from human liver was screened for 뱉-antitrypsin, a major constituent of plasma which functions as inhibitor of proteolytic enzyms. The library was screened using a 12-base-long synthetic oligodeoxyribonucleotide corresponding to a known DNA fragment of human 뱉-antitrypsin and by hybrid-selection of 뱉-antitrypsin mRNA