20 research outputs found
Environmentally induced changes in antioxidant phenolic compounds levels in wild plants
[EN] Different adverse environmental conditions cause oxidative stress in plants by generation of reactive
oxygen species (ROS). Accordingly, a general response to abiotic stress is the activation of enzymatic and non-enzymatic antioxidant systems. Many phenolic compounds, especially flavonoids, are known antioxidants and efficient ROS scavengers in vitro, but their exact role in plant stress responses in nature is still under debate. The aim of our work is to investigate this role by correlating the degree of
environmental stress with phenolic and flavonoid levels in stress-tolerant plants. Total phenolic and antioxidant flavonoid contents were determined in 19 wild species. Meteorological data and plant and soil samples were collected in three successive seasons from four Mediterranean ecosystems: salt marsh, dune, semiarid and gypsum habitats. Changes in phenolic and flavonoid levels were correlated
with the environmental conditions of the plants and were found to depend on both the taxonomy and ecology of the investigated species. Despite species-specific differences, principal component analyses of the results established a positive correlation between plant phenolics and several environmental parameters, such as altitude, and those related to water stress: temperature, evapotranspiration, and soil water deficit. The correlation with salt stress was, however, very weak. The joint analysis of all the species showed the lowest phenolic and flavonoid levels in the halophytes from the salt marsh. This finding supports previous data indicating that the halophytes analysed here do not undergo oxidative stress in their natural habitat and therefore do not need to activate antioxidant systems as a defence against salinity.This work has been funded by the Spanish Ministry of Science and Innovation (Project CGL2008-00438/BOS), with contribution from the European Regional Development Fund. Thanks to Dr. Rafael Herrera for critical reading of the manuscript.Bautista, I.; Boscaiu, M.; Lidón, A.; Llinares Palacios, JV.; Lull, C.; Donat-Torres, MP.; Mayoral García-Berlanga, O.... (2016). Environmentally induced changes in antioxidant phenolic compounds levels in wild plants. Acta Physiologiae Plantarum. 38(1):1-15. https://doi.org/10.1007/s11738-015-2025-2S115381Agati G, Biricolti S, Guidi L, Ferrini F, Fini A, Tattini M (2011) The biosynthesis of flavonoids is enhanced similarly by UV radiation and root zone salinity in L. vulgare leaves. 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Synthesis of Endogenous Compounds Labeled with for Positron Emission Tomography
Among the β⁺ emitting isotopes, produced in medical cyclotrons, carbon-11 is promising radionuclide for labeling of biologically active compounds and tracking their distribution in living organism. Therefore aminoacids, carboxylic acids, fatty acids and amines have been widely used in assessing and staging of brain tumors (-methionine), prostate cancer (-choline, -acetate) and neurological disorders (-labeled neurotransmitters). This paper reviews recent achievements in synthesis of endogenous substances labeled with and presents our experience in -methionine synthesis. Proposed protocol is a reliable tool for routine manufacturing in clinical applications and animal studies
Synthesis of 11C-methionine through gas phase iodination using Synthra MeIPlus synthesis module
A method of 11C-methionine synthesis using ‘bubbling’ method is presented. 11C-methionine was synthesized
via 11C methylation from L-cysteine thiolactone (2 mg) in a 300 L solution of 2:1:1 (v/v) 1 M NaOH,
ethanol, and water at ambient temperature (85°C, 5 min). The radiochemical purity of radiotracer was higher
than 99% and enantiomeric purity (L-11C-methionine) was 91.6 ± 0.4%. The fi nal product met the requirements
of European Pharmacopoeia monograph. The proposed 11C-methionine synthesis is a reliable tool for routine
manufacturing in clinical applications and animal studies
The Radiopharmaceuticals Production and Research Centre established by the Heavy Ion Laboratory of the University of Warsaw
The Radiopharmaceuticals Production and Research Centre was recently installed on the premises of the Heavy Ion Laboratory, University of Warsaw. Equipped with a medical PETtrace p/d cyclotron , radiochemistry synthesis and dispensing units and a modern quality control laboratory the Centre is intended to produce regularly for commercial purposes the classic PET radiopharmaceuticals ( such -as e.g. FDG- ). Situated on the largest Warsaw scientific campus OCHOTA, an important part of the Centre’s activities will also be devoted to the production of known species for preclinical studies and research into innovative radiopharmaceuticals in collaboration with other scientific units of this Campus as well as with members of the Warsaw Consortium for PET Collaboration. Research into the accelerator production route of 99mTc will also begin shortly