THE DISEASES AND PESTS CONTROL IN THE FARMING CULTURES, USING BIOPRODUCTS

The making of a performant agriculture is influenced among other things by the applicative working technologies, the phyto-sanitary protection having an important role within these technologies.The current research and studies concerning the methods and equipments of applying of phyto-sanitary treatments, are enrolled in the new trends for applying a long lasting agriculture; the phyto-sanitary protection represents one of the main sources of the environment poluation resources.In the first part of the work is presented a syntesis of the aspects concerning the impact of the phytosanitary treatments used in agriculture upon environment, the methods used these days for diseases and pests control and the future  trends  with referrence to the technologies and products used in  the agricultural ecological practice and at the end are presented products already used or being at the begining using phase

Deregulated expression of TANK in glioblastomas triggers pro-tumorigenic ERK1/2 and AKT signaling pathways

Signal transmission by the noncanonical IkappaB kinases (IKKs), TANK-binding kinase 1 (TBK1) and IKKɛ, requires interaction with adapter proteins such as TRAF associated NF-κB activator (TANK). Although increased expression or dysregulation of both kinases has been described for a variety of human cancers, this study shows that deregulated expression of the TANK protein is frequently occurring in glioblastomas (GBMs). The functional relevance of TANK was analyzed in a panel of GBM-derived cell lines and revealed that knockdown of TANK arrests cells in the S-phase and prohibits tumor cell migration. Deregulated TANK expression affects several signaling pathways controlling cell proliferation and the inflammatory response. Interference with stoichiometrically assembled signaling complexes by overexpression or silencing of TANK prevented constitutive interferon-regulatory factor 3 (IRF3) phosphorylation. Knockdown of TANK frequently prevents constitutive activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). TANK-mediated ERK1/2 activation is independent from the canonical MAP kinase or ERK kinase (MEK) 1/2-mediated pathway and utilizes an alternative pathway that uses a TBK1/IKKɛ/Akt signaling axis, thus identifying a novel pathway suitable to block constitutive ERK1/2 activity.Peer reviewe

CONSTRUCTION TYPES OF TECHNICAL EQUIPMENT FOR DOSING, WEIGHING, PACKAGING AND PROCEDURES USED IN MILLING UNITS

The paper presents several types of technical equipment for dosing, weighing, packaging and the procedures used in the milling units. The weighing systems take into account several important criteria for their design, namely: how the weight or mass of the load is offset and how the result is transferred

Gastric juice MicroRNAs as biomarkers for functional dyspepsia: an observational case-control study

Background and aimsMicroRNAs (miRNAs, miRs) are stable RNA molecules that regulate gene expression and hold promise as biomarkers. Functional dyspepsia (FD), a complex gastrointestinal disorder, is characterized by motility dysfunction, visceral hypersensitivity, and gut microbiome alterations. Given the limitations of current diagnostic markers, miRNAs may offer novel insights for diagnosis and risk stratification.MethodsWe conducted an observational case–control study involving 28 FD patients (14 with epigastric pain syndrome [EPS], 14 with postprandial distress syndrome [PDS]) and 22 healthy controls (HC). All subjects underwent gastroscopy with gastric juice collection. Quantitative real-time PCR was used to measure levels of selected miRNAs (miR-21-5p, miR-155-5p, miR-203a) in gastric fluid, with miR-16 and U6 as endogenous controls. Fold-change in miRNA expression was calculated. We compared miRNA levels between groups and between FD subtypes and assessed correlations with symptom severity (using the Functional Dyspepsia Symptom Diary scores). Statistical significance was determined by non-parametric tests, with p < 0.05 as threshold.ResultsGastric juice miR-21-5p was significantly elevated in FD patients compared to controls (FD: 19.98 ± 91.56 fold-change vs. HC: 2.63 ± 3.30 fold-change p = 0.00774). In contrast, miR-155-5p and miR-203a levels did not differ significantly overall between FD and healthy groups. However, within FD, PDS patients showed a distinct profile: markedly higher miR-21 and miR-155 but lower miR-203 relative to EPS patients. MiRNA levels correlated with symptom patterns: miR-21 and miR-155 levels were inversely correlated with epigastric pain intensity (τ = −0.517 and −0.317, respectively) but positively correlated with postprandial fullness and early satiety (τ up to 0.523, p < 0.01). Conversely, miR-203 showed direct correlation with epigastric pain (τ = 0.317, p = 0.023) and inverse correlation with fullness (τ = −0.523, p < 0.01). A history of Helicobacter pylori infection was associated with a significant reduction in gastric juice miR-203 levels (p ∼ 0.03).ConclusionFD patients might have altered gastric juice miRNA profiles, notably an upregulation of miR-21, and distinctive differences between PDS and EPS subtypes. The PDS subtype is characterized by a high-miR-21 and miR-155 and low-miR-203 signature, whereas the opposite pattern is observed in EPS. These miRNA alterations align with the symptomatology of FD subtypes and may reflect underlying pathophysiological differences. Gastric juice miRNAs could serve as minimally invasive biomarkers for FD, aiding in differentiating functional subgroups and potentially guiding targeted therapies. Further studies are warranted to confirm this findings and establish their diagnostic utility and role in FD pathogenesis

Profunda femoris reconstruction – a solution for inferior limb revascularization

Clinica Chirurgie II, Spitalul Clinic Judeţean de Urgenţă Mureş, Clinica Medicală II, Spitalul Clinic Judeţean de Urgenţă Mureş, Clinica Chirurgie Cardiovasculară II, IBCVT Tg. MureşProfunda femoris rămâne singura sursă vasculară pentru salvarea membrului inferior în cazurile leziunilor avansate ocluzive ale arterei femurale superficiale; există diverse proceduri chirurgicale, descrise sub numele de profundoplastii, preferinţa pentru o metodă sau alta bazându-se pe experienţă, situaţie anatomică intraoperatorie, preferințele chirurgului. În toate cazurile rezultatul reconstrucţiei este în strictă dependenţă de calitatea runoff-ului. Calibrul redus al vasului, absenţa colateralelor şi/sau ocluzia vaselor distale întunecă pronosticul postoperator sau chiar contraindică metoda. Scopul acestei lucrări este de a analiza rezultatele obţinute după profundoplastiile efectuate de autori în serviciul nostru.Profunda femoris remains the single vascular source for limb salvage in cases with superficial femoral artery obstruction; there are a lot of surgical procedures described under the name of profundoplasty, using one or another being a matter of experience, pre- or intraop anatomical findings, surgeons preference. The postop outcome depends on the quality of the run-off-poor collateralization or distal vascular obstructions are the worst predictive factors for surgical failure. The aim of this clinical retrospective study is to analyze the authors experience regarding this issue

Damage control surgery in abdominal traumatology. Three cases report

Clinica Chirurgie II, Spitalul Clinic Judeţean de Urgenţă MureşScopul principal al studiului clinic este de a accentua importanța chirurgiei de control lezional în traumele majore abdominale. Cu toate că nu am realizat un studiu retrospectiv al experienţei noastre în domeniu, am considerat util să prezentăm trei situaţii clinice în care pentru leziuni diferite chirurgia de control lezional a fost urmată de rezultate favorabile, atrăgând atenția asupra indicațiilor, criteriilor clinice, paraclinice și operatorii care determină utilizarea metodei.The main purpose of this clinical study is to emphasize the importance of the damage control surgery in major abdominal trauma. Although we do not made a statistical retrospective analysis of our experience in this issue, we describe here three of our cases where we performed damage control surgery with good postoperative results, drawing attention upon indications, clinical, paraclinical and operative criteria which indicate the method

HuR Controls Glutaminase RNA Metabolism

Glutaminase (GLS) is directly related to cell growth and tumor progression, making it a target for cancer treatment. The RNA-binding protein HuR (encoded by the ELAVL1 gene) influences mRNA stability and alternative splicing. Overexpression of ELAVL1 is common in several cancers, including breast cancer. Here we show that HuR regulates GLS mRNA alternative splicing and isoform translation/stability in breast cancer. Elevated ELAVL1 expression correlates with high levels of the glutaminase isoforms C (GAC) and kidney-type (KGA), which are associated with poor patient prognosis. Knocking down ELAVL1 reduces KGA and increases GAC levels, enhances glutamine anaplerosis into the TCA cycle, and drives cells towards glutamine dependence. Furthermore, we show that combining chemical inhibition of GLS with ELAVL1 silencing synergistically decreases breast cancer cell growth and invasion. These findings suggest that dual inhibition of GLS and HuR offers a therapeutic strategy for breast cancer treatment